FastQCFastQC Report
Fri 17 Jun 2016
SRR1526649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146364
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11110.7590664371020196No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10760.7351534530348993No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.6565822196715039No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4680.319750758383209No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.3156513896859884No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4060.27739061517859587No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3750.25621054357628925No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3200.21863299718510015No Hit
ACGTTGGGGAGTGGTGGTATGAGTGCCCAATCTCAACGAAACAACCAAGGCCACCTGTTCCCAT3150.21521685660408296No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.21453362848787955No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2800.19130387253696265No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.16944057281845262No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2390.16329151977262168No Hit
ACGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2310.1578256948429942No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2160.14757727309994262No Hit
ACGTTGGGGATTAACACTCTGAATTTATTTATTTTTTTATGTTTTTCTGTACTGAAGAAGGGAG2130.14552758875133232No Hit
ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2040.13937853570550135No Hit
ACGTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1850.12639720149763603No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1790.1222978328004154No Hit
ACGTTGGGATTGCACATCTTTTTATGAAATCTAGACTATGAAAAGGTGATAAACATGAAGTAAC1700.11614877975458446No Hit
ACGTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1700.11614877975458446No Hit
ACGTTGGGGGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCCTAGGACTGGAA1690.11546555163838101No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1640.11204941105736382No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG1630.11136618294116041No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1620.11068295482495696No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCCCTCCCC1610.10999972670875352No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1580.1079500423601432No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1520.10385067366292257No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1490.10180098931431227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAT258.419816E-658.24371758
TAGGACT201.9633843E-458.24371754
AAGTAAC303.6319398E-758.24371358
CCGTCAT303.6319398E-758.24371354
TGACCGT150.00460197658.24371352
ATATAAG150.00460197658.24371358
ATCGGCC150.00460197658.24371355
AATGCGA150.004608253558.22367542
ATGCGAA150.004608253558.22367543
AACTCCT150.004608253558.22367543
AACTCCA150.004608253558.22367545
CGCGTTA150.004608253558.22367543
TCGCGTG150.004608253558.22367545
CCCGACG150.004608253558.22367542
ACACCCT150.004620828758.1836339
ATCGTCT150.004620828758.1836340
CAAATCA150.004620828758.1836340
CTCGCGT150.004620828758.1836341
CGATCTC201.9734337E-458.1836339
TTACATA150.004620828758.1836340
Produced by FastQC (version 0.11.5)