FastQCFastQC Report
Fri 17 Jun 2016
SRR1526648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142005
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8710.6133586845533608No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8200.5774444561811204No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.5668814478363438No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.3084398436674765No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4270.3006936375479737No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3240.2281609802471744No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3190.22463997746558223No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2940.20703496355762124No Hit
ACGTTGGGGAGTGGTGGTATGAGTGCCCAATCTCAACGAAACAACCAAGGCCACCTGTTCCCAT2720.19154255131861553No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2600.18309214464279427No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.17816274074856517No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2000.1408401112636879No Hit
ACGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1930.13591070736945882No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.13027710291891131No Hit
ACGTTGGGGATTAACACTCTGAATTTATTTATTTTTTTATGTTTTTCTGTACTGAAGAAGGGAG1750.12323509735572692No Hit
ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1710.12041829513045316No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1590.11196788845463188No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1520.1070384845604028No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1480.10422168233512905No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG1460.10281328122249216No Hit
ACGTTGGGGGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCCTAGGACTGGAA1450.10210908066617373No Hit
ACGTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1450.10210908066617373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACA351.5557816E-858.33202737
TACATGT201.9519439E-458.31125636
ACGAAAC351.5601472E-858.31125336
TTTACGG201.9553848E-458.29049750
ATATCGA150.00458705658.29049750
GATATCG150.00459352858.26975649
GGTTACT150.00459352858.26975649
AATTTGG150.004600006658.24902358
GTAGGGC303.628793E-758.24902333
TCTAGTT150.004600006658.24902357
TACGGCA150.004600006658.24902352
TCGATAT150.004600006658.24902347
ATGAGTA150.004600006658.24902334
AGTGAAT150.004600006658.24902334
GTACTTC150.004600006658.24902358
CATAATA150.004600006658.24902358
TATCGAC150.004600006658.24902351
TGACTAT150.004600006658.24902333
CGGGTGA258.413865E-658.2490233
TGTTAGA258.413865E-658.2490257
Produced by FastQC (version 0.11.5)