##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526648_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142005 Sequences flagged as poor quality 0 Sequence length 64 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.07069469384881 39.0 38.0 40.0 35.0 40.0 2 37.819309179254255 39.0 38.0 40.0 35.0 40.0 3 37.88865885004049 39.0 38.0 40.0 35.0 40.0 4 37.616605049117986 39.0 38.0 40.0 33.0 40.0 5 38.038308510263725 39.0 38.0 40.0 35.0 40.0 6 37.630970740466886 39.0 38.0 40.0 33.0 40.0 7 38.064138586669486 39.0 38.0 40.0 35.0 40.0 8 37.92425618816239 39.0 38.0 40.0 35.0 40.0 9 38.008309566564556 39.0 38.0 40.0 35.0 40.0 10 37.32062955529735 39.0 38.0 40.0 33.0 40.0 11 37.32685468821521 39.0 37.0 40.0 33.0 40.0 12 37.23999154959333 39.0 37.0 40.0 32.0 40.0 13 37.24625189253899 39.0 37.0 40.0 33.0 40.0 14 37.19764092813633 39.0 37.0 40.0 33.0 40.0 15 37.02111897468399 39.0 37.0 40.0 33.0 40.0 16 36.72668568008169 39.0 36.0 40.0 32.0 40.0 17 36.38677511355234 38.0 36.0 40.0 31.0 40.0 18 36.648991232703075 38.0 36.0 40.0 31.0 40.0 19 36.517573324882925 38.0 36.0 40.0 31.0 40.0 20 36.382669624309 38.0 35.0 40.0 31.0 40.0 21 36.46386394845252 38.0 36.0 40.0 31.0 40.0 22 36.3611140452801 38.0 36.0 40.0 31.0 40.0 23 36.254040350691874 38.0 35.0 40.0 30.0 40.0 24 36.11231294672723 38.0 35.0 40.0 30.0 40.0 25 36.016421956973346 38.0 35.0 40.0 30.0 40.0 26 36.22035843808317 39.0 36.0 40.0 30.0 40.0 27 35.663504806168795 39.0 35.0 40.0 29.0 40.0 28 35.54718495827612 38.0 35.0 40.0 28.0 40.0 29 35.41533044611105 38.0 35.0 40.0 28.0 40.0 30 35.3566564557586 38.0 35.0 40.0 28.0 40.0 31 34.8671384810394 38.0 35.0 40.0 26.0 40.0 32 35.01788669413049 38.0 35.0 39.0 27.0 40.0 33 34.90865814583994 38.0 35.0 39.0 27.0 40.0 34 34.262420337312065 38.0 33.0 39.0 23.0 40.0 35 34.30069363754797 38.0 33.0 39.0 24.0 40.0 36 33.69453892468575 38.0 33.0 39.0 20.0 40.0 37 33.42890039083131 38.0 33.0 39.0 19.0 40.0 38 33.405626562444986 38.0 33.0 39.0 20.0 40.0 39 32.907235660716175 38.0 32.0 39.0 15.0 40.0 40 33.054582585120244 38.0 32.0 39.0 17.0 40.0 41 32.747508890532025 38.0 32.0 39.0 10.0 40.0 42 32.429745431498894 37.0 32.0 39.0 7.0 40.0 43 32.41049963029471 37.0 32.0 39.0 8.0 40.0 44 32.26007534945953 37.0 31.0 39.0 4.0 40.0 45 32.094151614379776 37.0 31.0 39.0 4.0 40.0 46 31.75077638111334 36.0 31.0 39.0 4.0 40.0 47 31.537319108482095 36.0 31.0 39.0 4.0 40.0 48 31.431083412555896 36.0 30.0 39.0 4.0 40.0 49 31.179155663532974 36.0 30.0 39.0 4.0 40.0 50 30.824224499137355 36.0 30.0 39.0 4.0 40.0 51 30.44211119326784 36.0 29.0 39.0 4.0 40.0 52 30.305390655258616 35.0 29.0 39.0 4.0 40.0 53 30.048751804513927 35.0 29.0 39.0 4.0 40.0 54 29.821590789056724 35.0 28.0 39.0 4.0 40.0 55 29.557128270131333 35.0 27.0 39.0 4.0 40.0 56 28.933664307594803 35.0 26.0 39.0 4.0 40.0 57 28.517171930565826 35.0 25.0 38.0 4.0 40.0 58 28.222140065490652 34.0 24.0 38.0 4.0 40.0 59 27.87698320481673 34.0 23.0 38.0 4.0 39.0 60 27.59469032780536 34.0 21.0 38.0 4.0 39.0 61 27.150297524735045 34.0 13.0 38.0 4.0 39.0 62 26.86667370867223 33.0 4.0 38.0 4.0 39.0 63 26.558283159043697 33.0 4.0 38.0 4.0 39.0 64 26.533544593500228 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.3090256578544128 2 2 0.11591217280236776 2 3 -0.2420713914407031 2 4 0.08260622258943329 2 5 -0.19094950344388906 2 6 -0.38799667004811056 2 7 -0.0010202393636973284 2 8 0.229260330174462 2 9 -0.05510246997600632 2 10 0.20023286728633138 2 11 -0.30557167881800495 2 12 -0.3901902127218335 2 13 -0.21040630160580776 2 14 -0.27464251921725236 2 15 0.08689049511058755 2 16 -0.25834092482091364 2 17 0.35191028441309413 2 18 0.24502988515345692 2 19 -0.707997851043416 2 20 -0.3336876931109032 2 21 0.12066415724072499 2 22 -0.059356052756243116 2 23 -0.1689333890111513 2 24 -0.9190064186195102 2 25 -0.3406896114471536 2 26 0.19603335368292818 2 27 0.43699301004992464 2 28 0.2678972841773941 2 29 0.3264834882744836 2 30 0.20729886351672633 2 31 0.6935675457053279 2 32 0.7464705659378126 2 33 0.04268505140314005 2 34 0.6214025507320002 2 35 -0.25189198354291165 2 36 -1.7593043431202773 2 37 -2.1078850471292583 2 38 -2.1526464739057225 2 39 -4.749617649751201 2 40 -1.7574437752056618 2 41 -1.2883290702132335 2 42 -1.7548234082614904 2 43 -1.9747894020158618 2 44 -1.4719865375751553 2 45 -1.8312510455290614 2 46 -1.125981440190781 2 47 -1.3925532317584128 2 48 -1.459173754238087 2 49 -1.7339571774193523 2 50 -0.8905749563483703 2 51 -1.1877117578650989 2 52 -1.5018476412586246 2 53 -1.3746615491236973 2 54 -1.505297718869059 2 55 -1.5422567841696555 2 56 -1.8752331564222686 2 57 -2.157698958305307 2 58 -1.535019937760989 2 59 -1.5817952097062786 2 60 -1.3276714370403475 2 61 -1.6591231400724453 2 62 -0.6918418854559434 2 63 -0.5996728824566695 2 64 -1.1056482661440263 3 1 -0.1982334649726667 3 2 0.06576083160625501 3 3 -0.39998810159622167 3 4 -0.2102128985878977 3 5 -0.3884236710673079 3 6 -0.4094975572206394 3 7 -0.13122585464394376 3 8 -0.021757308902877526 3 9 -0.25205477140591626 3 10 -0.24418838310506885 3 11 -0.39935103379139036 3 12 -0.12910055610978333 3 13 -0.39744314535906966 3 14 -0.09094561910244181 3 15 -0.08939590505640638 3 16 0.05624001662570066 3 17 0.7281758099323525 3 18 -0.302146818739665 3 19 -0.1484007317448004 3 20 -0.3393238480007881 3 21 0.053708724613649395 3 22 -0.4995501869802297 3 23 -0.2188237971105096 3 24 -0.11762869186864577 3 25 -0.1267766587282253 3 26 0.28062786365057235 3 27 0.4519705698035992 3 28 0.6847639995515777 3 29 0.9168185263707116 3 30 0.6403956175090002 3 31 0.6149744969630291 3 32 0.778043470645045 3 33 1.1214346600034943 3 34 0.7752487045781535 3 35 0.40665096604481477 3 36 -4.344264159423378 3 37 -3.903939738706338 3 38 -3.804144229881093 3 39 -20.30039523037953 3 40 -5.894485837620859 3 41 -4.598787268731133 3 42 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0.3540053409974817 119 2 0.7150145629495341 119 3 0.2791163760157218 119 4 0.74501098200912 119 5 0.35038229908194296 119 6 0.7099054278539896 119 7 0.592654742370982 119 8 0.7543620940821967 119 9 0.5837661241164724 119 10 0.3379011691337368 119 11 0.7872161303877121 119 12 0.2514964588155877 119 13 0.2965867053871989 119 14 0.14786035104680906 119 15 0.6120966322570283 119 16 0.7129564345361459 119 17 1.3177280487383243 119 18 0.5038233305140665 119 19 0.9411515070611713 119 20 0.6159000325962296 119 21 1.0984848440166388 119 22 0.8586587682436502 119 23 0.8781911282626211 119 24 0.5987892185791139 119 25 0.5224770726150538 119 26 1.4597323412625087 119 27 1.683313853385691 119 28 1.2967042980590406 119 29 1.5212961383110155 119 30 1.5881568115388518 119 31 1.7567655417391492 119 32 1.5765509333316103 119 33 1.718449585376625 119 34 1.6782337792050157 119 35 1.5335166376866098 119 36 2.0437955420691623 119 37 1.931881156816047 119 38 1.718243829820402 119 39 2.266768948724952 119 40 2.083126102677646 119 41 1.7967351193285666 119 42 2.361604939510137 119 43 2.162983273070246 119 44 1.8310611609949348 119 45 2.3344414709097236 119 46 1.9027733870715124 119 47 2.3136361012965914 119 48 2.170927487803457 119 49 2.2279987770739176 119 50 2.6947501662901843 119 51 1.5633058812122407 119 52 2.5400060274607164 119 53 2.3513586992846136 119 54 2.219156930958725 119 55 2.4843062050799034 119 56 0.5198195520632751 119 57 0.3844156435107884 119 58 0.47833983679281644 119 59 0.88146541027076 119 60 0.7349525061806084 119 61 2.10134027710739 119 62 2.4891934970719767 119 63 2.162877999497198 119 64 2.3054779211015664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 205.0 5 20.0 6 59.0 7 91.0 8 214.0 9 155.0 10 263.0 11 300.0 12 347.0 13 482.0 14 553.0 15 672.0 16 818.0 17 959.0 18 1003.0 19 1249.0 20 1309.0 21 1686.0 22 2106.0 23 1718.0 24 1935.0 25 1953.0 26 1904.0 27 2331.0 28 2800.0 29 3160.0 30 3791.0 31 4373.0 32 5498.0 33 6964.0 34 8682.0 35 11182.0 36 14528.0 37 19167.0 38 22199.0 39 17251.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.20425337136017 0.6070208795464949 0.08098306397662054 0.10774268511672125 8 98.78174234097122 0.7518108283306558 0.28888700994898675 0.17755982074913335 9 94.9473610084152 2.880884475898736 1.2647441991479174 0.9070103165381501 10 47.91169325023767 34.79173268546882 6.972993908665188 10.323580155628322 11 33.82627372275624 28.955318474701595 18.69934157247984 18.51906623006232 12 38.702862575261435 17.804302665399106 22.761170381324604 20.73166437801486 13 26.192739692264354 17.66416675469174 27.69409527833527 28.448998274708636 14 24.265342769620787 23.31678462025985 30.66300482377381 21.754867786345553 15 31.06627104667799 21.46073988455602 26.696596587425205 20.776392481340785 16 31.421467462123083 23.219908010678086 24.487754713924478 20.870869813274354 17 32.388278594897976 22.355562449762385 22.178585026722885 23.077573928616758 18 30.74539628886307 23.832963628041266 23.53297419104961 21.888665892046056 19 29.497552903066794 25.877257843033696 23.12242526671596 21.50276398718355 20 26.09773795182845 30.30693690076153 20.76632077266099 22.829004374749033 21 24.803351994648075 25.00897855709306 28.816590965106865 21.371078483152 22 25.570930601035176 24.376606457519102 25.22657652899546 24.825886412450267 23 28.598992993204465 24.88363085806838 24.231541142917504 22.285835005809655 24 28.07112676056338 27.738732394366195 22.95492957746479 21.23521126760563 25 28.70458436370554 21.71707564517152 23.10710939206279 26.471230599060156 26 25.97251667523613 21.3435979067032 25.182952168308955 27.500933249751718 27 23.21438649960492 21.33423636979343 25.777457952364824 29.673919178236822 28 23.226161343328844 27.869418958087973 23.27623292453296 25.628186774050228 29 29.221603327927802 24.904463089614325 24.53359655926109 21.340337023196785 30 26.44844979013086 25.15537370815844 26.880886035765933 21.515290465944766 31 26.796578349307627 23.933910195785046 28.517284699970357 20.752226754936974 32 29.59097320169252 21.88716502115656 29.7722143864598 18.749647390691116 33 27.890321260487006 23.45067490809537 29.01928409644165 19.639719734975973 34 22.947981371946263 26.811394489318552 33.281039100539196 16.95958503819599 35 20.605525953632803 31.960901937259607 31.14224213980733 16.291329969300257 36 23.30322015581627 31.348312225855896 27.753801818091922 17.594665800235916 37 23.891954526506183 29.367709297478328 29.53657450912508 17.203761666890408 38 26.061984726353042 28.237878927029836 28.459707102033892 17.240429244583225 39 24.194851586199075 26.29631729651575 28.048365605420578 21.460465511864598 40 23.655534040959395 24.837109380520968 30.51460715446695 20.99274942405269 41 19.73102731444491 26.929794169430167 30.435489687259153 22.903688828865775 42 20.133202160111544 25.013270672168392 31.039925259574346 23.813601908145714 43 20.91996320147194 22.587927252140684 33.80298634208478 22.689123204302597 44 22.99019677186629 23.712353764977152 30.902095033313998 22.395354429842556 45 22.029377219918775 24.082669421370653 30.419431983811396 23.468521374899176 46 21.14214132034877 25.184010870796058 30.496263163854604 23.177584645000564 47 20.1450916554604 25.831976785335126 32.663316582914575 21.3596149762899 48 22.373812067904073 26.660628232979754 30.88446542171147 20.081094277404702 49 23.41816946465476 25.328397927695832 31.035727429720012 20.217705177929393 50 20.08318394705703 24.357875195737357 32.413397291916134 23.14554356528948 51 18.606445948624998 27.762043671585147 32.32532782010252 21.306182559687333 52 18.63439367759114 26.82916466022718 33.06730306206271 21.46913860011897 53 20.255558467499167 25.15281801375539 33.57533945785906 21.01628406088638 54 19.262149949714576 25.772341600917887 31.562955932971654 23.40255251639588 55 21.232216743184264 26.358895879385592 29.131950348580173 23.27693702884997 56 20.927843087235956 27.376118704959502 27.52775947223344 24.168278735571096 57 21.73389353757643 28.667483827999344 28.13680130934752 21.461821325076695 58 21.24398250228647 31.027955220600223 26.876147667089693 20.85191461002361 59 23.108010379886842 30.834077792430627 27.59461720622226 18.463294621460275 60 25.49773386576258 27.322698933959387 27.74826404896836 19.431303151309677 61 24.744737084511335 25.855777338463394 27.46990342169221 21.929582155333062 62 21.43272283964618 25.924245860739397 27.952767067362217 24.69026423225221 63 20.97153710948574 28.215541191812665 26.562057039179 24.250864659522595 64 22.040619435754465 26.364326402633242 27.339731994012784 24.25532216759951 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 3.5 5 16.0 6 25.0 7 107.0 8 189.0 9 665.5 10 848.0 11 554.0 12 441.0 13 299.0 14 270.0 15 435.5 16 763.5 17 926.0 18 933.5 19 941.0 20 1101.0 21 1511.0 22 1761.0 23 1956.5 24 2498.0 25 2844.0 26 3025.5 27 3298.5 28 3390.0 29 3089.5 30 3165.0 31 3541.0 32 3097.0 33 2653.0 34 2371.5 35 2190.0 36 2290.0 37 2457.5 38 2651.0 39 2677.0 40 2548.5 41 2519.0 42 2618.0 43 2798.5 44 2979.0 45 3073.5 46 3766.5 47 4365.0 48 4035.0 49 3959.0 50 4213.0 51 4418.0 52 5241.5 53 5860.0 54 6398.0 55 7054.5 56 7173.0 57 7281.0 58 7389.0 59 8018.5 60 8956.0 61 9264.0 62 8733.0 63 6996.0 64 5790.0 65 5294.5 66 4096.5 67 3394.0 68 2951.5 69 2509.0 70 2126.0 71 1528.5 72 1314.0 73 1167.0 74 812.5 75 605.0 76 501.5 77 326.5 78 255.0 79 193.5 80 114.0 81 96.0 82 69.0 83 42.0 84 37.5 85 33.0 86 33.0 87 20.0 88 6.0 89 5.0 90 3.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0570402450617936 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08309566564557586 16 0.022534417802190065 17 0.12605189958100066 18 0.0 19 0.0 20 0.03802683004119573 21 0.0 22 0.0 23 0.0 24 0.0035210027815921973 25 0.046477236717017006 26 0.019717615576916307 27 0.1837963451991127 28 0.1464737157142354 29 0.12323509735572692 30 0.17605013907960987 31 0.22463997746558223 32 0.1443611140452801 33 0.1985845568817999 34 0.3506918770465829 35 0.21830217245871622 36 0.3013978381042921 37 0.3323826625823034 38 0.3197070525685715 39 0.6429351079187353 40 0.3513960776029013 41 0.407027921552058 42 0.5042075983240026 43 0.48941938664131546 44 0.43871694658638777 45 0.47251857328967295 46 0.4992781944297736 47 0.5035033977676843 48 0.48448998274708643 49 0.5020949966550473 50 0.6140628851096792 51 0.5415302278088799 52 0.5584310411605226 53 0.5802612584063942 54 0.5711066511742544 55 0.6063166789901764 56 0.6211048906728636 57 0.6098376817717686 58 0.674624132953065 59 0.6781451357346573 60 0.7154677652195346 61 0.616879687334953 62 0.645751910144009 63 0.6401183056934615 64 0.7309601774585401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 142005.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.22773845991339 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60520993447132 41.57600084504067 2 8.425693714100802 8.801098552867858 3 3.811719655907019 5.972324918136685 4 2.173502683170186 4.540685187141298 5 1.4710244586468193 3.8414140347170873 6 1.0206833319850066 3.1984789267983524 7 0.7200064719682873 2.632301679518327 8 0.4934875819108487 2.0618992289003906 9 0.3883180972413235 1.8252878419773952 >10 1.7325998435940997 15.603675926903982 >50 0.11325944502871936 4.128727861695011 >100 0.0404498017959712 4.060420407732122 >500 0.00404498017959712 1.757684588570825 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 871 0.6133586845533608 No Hit ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 820 0.5774444561811204 No Hit ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 805 0.5668814478363438 No Hit ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 438 0.3084398436674765 No Hit ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 427 0.3006936375479737 No Hit ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 324 0.2281609802471744 No Hit ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 319 0.22463997746558223 No Hit ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 294 0.20703496355762124 No Hit ACGTTGGGGAGTGGTGGTATGAGTGCCCAATCTCAACGAAACAACCAAGGCCACCTGTTCCCAT 272 0.19154255131861553 No Hit ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 260 0.18309214464279427 No Hit ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 253 0.17816274074856517 No Hit ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 200 0.1408401112636879 No Hit ACGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 193 0.13591070736945882 No Hit ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.13027710291891131 No Hit ACGTTGGGGATTAACACTCTGAATTTATTTATTTTTTTATGTTTTTCTGTACTGAAGAAGGGAG 175 0.12323509735572692 No Hit ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA 171 0.12041829513045316 No Hit ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 159 0.11196788845463188 No Hit ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 152 0.1070384845604028 No Hit ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 148 0.10422168233512905 No Hit ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG 146 0.10281328122249216 No Hit ACGTTGGGGGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCCTAGGACTGGAA 145 0.10210908066617373 No Hit ACGTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 145 0.10210908066617373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0021126016689553186 0.0 0.0 0.0 0.0 8 0.0035210027815921973 0.0 0.0 0.0 0.0 9 0.004929403894229077 0.0 0.0 0.0 0.0 10 0.017605013907960987 0.0 0.0 0.0 0.0 11 0.029576423365374457 0.0 0.0 0.0 0.0 12 0.0302806239216929 0.0 0.0 0.0 0.0 13 0.03168902503432978 0.0 0.0 0.0 0.0 14 0.039435231153832614 0.0 0.0 0.0 0.0 15 0.04084363226646949 0.0 0.0 0.0 0.0 16 0.04295623393542481 0.0 0.0 0.0 0.0 17 0.04788563782965388 0.0 0.0 0.0 0.0 18 0.05422344283651984 0.0 0.0 0.0 0.0 19 0.061265448399704235 0.0 0.0 0.0 0.0 20 0.07816626175134678 0.0 0.0 0.0 0.0 21 0.08309566564557586 0.0 0.0 0.0 0.0 22 0.0999964789972184 0.0 0.0 0.0 0.0 23 0.11196788845463188 0.0 0.0 0.0 0.0 24 0.12534769902468224 0.0 0.0 0.0 0.0 25 0.13591070736945882 0.0 0.0 0.0 0.0 26 0.14717791627055385 0.0 0.0 0.0 0.0 27 0.1640787296221964 0.0 0.0 0.0 0.0 28 0.19224675187493398 0.0 0.0 0.0 0.0 29 0.19506355410020773 0.0 0.0 0.0 0.0 30 0.20421816133234746 0.0 0.0 0.0 0.0 31 0.20703496355762122 0.0 0.0 0.0 0.0 32 0.21126016689553184 0.0 0.0 0.0 0.0 33 0.2175979719023978 0.0 0.0 0.0 0.0 34 0.22323157635294533 0.0 0.0 0.0 0.0 35 0.2654836097320517 0.0 0.0 0.0 0.0 36 0.2704130136262808 0.0 0.0 0.0 0.0 37 0.2767508186331467 0.0 0.0 0.0 0.0 38 0.28097602197105737 0.0 0.0 0.0 0.0 39 0.28660962642160487 0.0 0.0 0.0 0.0 40 0.296468434210063 0.0 0.0 0.0 0.0 41 0.3006936375479737 0.0 0.0 0.0 0.0 42 0.3119608464490687 0.0 0.0 0.0 0.0 43 0.3211154536812084 0.0 0.0 0.0 0.0 44 0.3267490581317559 0.0 0.0 0.0 0.0 45 0.3351994648075772 0.0 0.0 0.0 0.0 46 0.34083306925812473 0.0 0.0 0.0 0.0 47 0.34857927537762756 0.0 0.0 0.0 0.0 48 0.35562128094081197 0.0 0.0 0.0 0.0 49 0.3640716876166332 0.0 0.0 0.0 0.0 50 0.37040949262349915 0.0 0.0 0.0 0.0 51 0.37674729763036513 0.0 0.0 0.0 0.0 52 0.38378930319354954 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAACA 35 1.5557816E-8 58.332027 37 TACATGT 20 1.9519439E-4 58.311256 36 ACGAAAC 35 1.5601472E-8 58.311253 36 TTTACGG 20 1.9553848E-4 58.290497 50 ATATCGA 15 0.004587056 58.290497 50 GATATCG 15 0.004593528 58.269756 49 GGTTACT 15 0.004593528 58.269756 49 AATTTGG 15 0.0046000066 58.249023 58 GTAGGGC 30 3.628793E-7 58.249023 33 TCTAGTT 15 0.0046000066 58.249023 57 TACGGCA 15 0.0046000066 58.249023 52 TCGATAT 15 0.0046000066 58.249023 47 ATGAGTA 15 0.0046000066 58.249023 34 AGTGAAT 15 0.0046000066 58.249023 34 GTACTTC 15 0.0046000066 58.249023 58 CATAATA 15 0.0046000066 58.249023 58 TATCGAC 15 0.0046000066 58.249023 51 TGACTAT 15 0.0046000066 58.249023 33 CGGGTGA 25 8.413865E-6 58.24902 33 TGTTAGA 25 8.413865E-6 58.24902 57 >>END_MODULE