FastQCFastQC Report
Fri 17 Jun 2016
SRR1526647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129982
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10470.8054961456201628No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10440.803188133741595No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8480.652398024341832No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7000.5385361049991537No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5390.4146728008493483No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4960.3815912972565432No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3950.3038882306780939No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3470.26696004062100903No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3010.2315705251496361No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2480.1907956486282716No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.18464095028542413No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2320.17848625194257667No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2280.17540890277115292No Hit
ACGTTGGGGAGTGGTGGTATGAGTGCCCAATCTCAACGAAACAACCAAGGCCACCT2240.17233155359972918No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.16463818067116984No Hit
ACGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1870.14386607376405963No Hit
ACGTTGGGGATTAACACTCTGAATTTATTTATTTTTTTATGTTTTTCTGTACTGAA1700.13078733978550877No Hit
ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1620.12463264144266128No Hit
ACGTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1590.12232462956409348No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1590.12232462956409348No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1580.12155529227123757No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1580.12155529227123757No Hit
ACGTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1570.12078595497838163No Hit
ACGTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1550.11924728039266975No Hit
ACGTTGGGGGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCTTCCC1530.11770860580695788No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.10386053453555108No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1320.10155252265698328No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1320.10155252265698328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTG150.00800467150.6348337
TAATGTA203.9333414E-450.59522636
GCGTTGC150.00805434950.55568335
AGACGGC150.00805434950.55568335
CGCTCTA203.9485568E-450.55568334
GTGTCGG203.9485568E-450.55568335
TGTGTCG203.9485568E-450.55568334
GTAGGAT251.9681655E-550.457133
CCGCGTG150.00811676950.45709633
ACCGACG251.98184E-550.3981331
GTACTTA204.0098882E-450.3981331
TACTCAA150.00815439250.3981331
CCGACGA251.98184E-550.3981332
AGCCATT204.0876144E-450.2025648
CCTACCA150.00828074450.20255748
CAAATTG150.00828074450.20255748
ACTAAGA150.00828074450.20255748
TACTAAG150.00828074450.20255747
TCAAATT150.00828074450.20255747
CCCGAGC150.00828074450.20255747
Produced by FastQC (version 0.11.5)