FastQCFastQC Report
Fri 17 Jun 2016
SRR1526646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185118
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC43370.36595512008086956No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC37100.3130489959649588No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC29580.24959539893917737No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT25140.21213077516331708No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC16870.14234869439161332No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT16830.14201117525849746No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT15570.1313793225653479No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG14190.1197349124728508No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC13940.1176254178908767No Hit
TCTACCGGGGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAACATTCTTCCC12490.10539034931542682No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC12480.10530596953214787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT201.9733037E-458.2189157
TACCGGG1119750.057.1258433
CTACCGG1181450.057.1210562
TCTACCG1193500.057.118531
CCGGGAT207150.056.951425
ACCGGGG341000.056.92354
ACCGGGA630150.056.8763774
TACCGGA40600.056.6912963
CGGGATT67250.056.6699036
TACCGGT11000.056.5164763
ACCGGGT62950.056.406864
CGGGATG58300.056.3428846
CGGGATA25100.056.3332026
CGGGTAT5500.056.253616
CGGGATC58450.056.2477656
CCGGGAG194300.056.0301175
CCGGGGT61100.055.89055
ACCGGGC92500.055.8301854
CCGGGAC110400.055.6045385
CCGGGGG163250.055.581355
Produced by FastQC (version 0.11.5)