FastQCFastQC Report
Fri 17 Jun 2016
SRR1526645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151625
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC35010.3040052100293064No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC30770.2671876695973081No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT27720.240703353956366No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC23580.20475415174210357No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT14320.12434603277976773No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC13380.11618365353305113No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT11860.10298491262346683No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG11610.10081406707912732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGG1082850.056.9689833
CTACCGG1142350.056.9671252
TCTACCG1158400.056.9383961
ACCGGGA600050.056.8439754
CCGGGAT194250.056.821785
CGGGATT67500.056.7743726
ACCGGGG334000.056.7208254
CGGGATA21600.056.4214026
CGGGATC51900.056.2560166
ACCGGGT63200.056.204784
CCGGGAG188100.056.1463285
CCGGGAC101450.055.9365545
TACCGGA41150.055.8635373
CGGGAGT45400.055.7864766
CGGGAGC55500.055.624996
CGGGATG55150.055.611456
CGGGAAC36450.055.381526
TACCGGT9650.055.3642773
CCGGGGT62400.055.3055275
ACCGGGC91950.055.2772144