FastQCFastQC Report
Fri 17 Jun 2016
SRR1526644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053738
Sequences flagged as poor quality0
Sequence length56
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC65910.625487550036157No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC41840.39706264745126396No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC30760.2919131700669426No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG22890.21722667304396348No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC20890.19824662297459142No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT18600.17651446564516038No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC17490.16598053785665887No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA13870.1316266472310954No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC12380.11748650992941319No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12220.11596810592386343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG252.1454975E-549.768286
CTACCGG1045500.049.158972
TCTACCG1057450.049.1517371
TACCGGG987500.049.1383063
CGGGACT34650.049.1219376
ACCGGGA543900.049.091164
TACCGGA40050.049.0226863
CCGGGAT174900.048.9573025
ACCGGGT58000.048.910214
ACCGGGG302450.048.904394
CGGGATA19700.048.884076
CGGGATT60700.048.825396
CCGGGAC90750.048.6714555
CGGGATG49850.048.520336
CCGGGAG176050.048.411355
CGGGATC45900.048.3045046
TACCGGT9200.048.1454053
ACCGGGC87400.048.0030444
TACCGGC9700.047.972523
CCGGGGT55650.047.9349485
Produced by FastQC (version 0.11.5)