##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526644_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1053738 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.78522365141999 39.0 39.0 40.0 37.0 40.0 2 38.54712271930973 39.0 39.0 40.0 36.0 40.0 3 38.52275233502066 39.0 39.0 40.0 35.0 40.0 4 38.49959762293853 39.0 39.0 40.0 36.0 40.0 5 38.41773381998182 39.0 39.0 40.0 36.0 40.0 6 38.76666021344964 39.0 39.0 40.0 38.0 40.0 7 38.61993114037835 39.0 39.0 40.0 38.0 40.0 8 38.831040543284956 39.0 39.0 40.0 38.0 40.0 9 38.75513362904251 39.0 39.0 40.0 38.0 40.0 10 37.965571138176664 39.0 38.0 40.0 35.0 40.0 11 38.23408475351558 39.0 38.0 40.0 35.0 40.0 12 38.09353843175438 39.0 38.0 40.0 35.0 40.0 13 38.04706198314951 39.0 38.0 40.0 35.0 40.0 14 38.022293017808984 39.0 38.0 40.0 35.0 40.0 15 38.04329823922075 39.0 38.0 40.0 35.0 40.0 16 37.94267740178299 39.0 38.0 40.0 35.0 40.0 17 37.86224943961402 39.0 38.0 40.0 35.0 40.0 18 37.79382541011143 39.0 38.0 40.0 34.0 40.0 19 37.71293338571827 39.0 38.0 40.0 33.0 40.0 20 37.6471418891603 39.0 38.0 40.0 33.0 40.0 21 37.55140651661039 39.0 38.0 40.0 33.0 40.0 22 37.5274147843202 39.0 38.0 40.0 33.0 40.0 23 37.36865710451744 39.0 37.0 40.0 33.0 40.0 24 37.288362951701465 39.0 37.0 40.0 33.0 40.0 25 37.19388975248117 39.0 37.0 40.0 33.0 40.0 26 36.82179346289116 39.0 36.0 40.0 32.0 40.0 27 36.6873871873274 39.0 36.0 40.0 32.0 40.0 28 36.53599661395907 38.0 36.0 40.0 31.0 40.0 29 36.40631447285758 38.0 36.0 40.0 31.0 40.0 30 36.16301205802581 38.0 35.0 40.0 31.0 40.0 31 35.551403669602884 38.0 35.0 40.0 29.0 40.0 32 35.44163349902917 38.0 35.0 39.0 29.0 40.0 33 35.23202067307054 38.0 35.0 39.0 28.0 40.0 34 35.52767291300115 38.0 35.0 40.0 29.0 40.0 35 35.364312571056566 38.0 35.0 40.0 29.0 40.0 36 34.74362697368796 38.0 35.0 40.0 27.0 40.0 37 34.27484441103956 38.0 34.0 40.0 24.0 40.0 38 34.364194894746134 38.0 34.0 40.0 25.0 40.0 39 34.260526810269724 38.0 33.0 40.0 25.0 40.0 40 33.945328914777676 38.0 33.0 39.0 23.0 40.0 41 33.51914897251499 38.0 33.0 39.0 21.0 40.0 42 33.45370291286829 38.0 33.0 39.0 21.0 40.0 43 33.32431970755539 37.0 33.0 39.0 20.0 40.0 44 32.902130320819786 37.0 32.0 39.0 16.0 40.0 45 32.81778108030649 37.0 32.0 39.0 17.0 40.0 46 32.33610157363595 36.0 32.0 39.0 4.0 40.0 47 32.120301251354704 36.0 31.0 39.0 4.0 40.0 48 31.75789522632761 36.0 31.0 39.0 4.0 40.0 49 31.72268913145393 36.0 31.0 39.0 4.0 40.0 50 31.43323577587598 36.0 31.0 39.0 4.0 40.0 51 30.905566658884847 36.0 30.0 38.0 4.0 40.0 52 30.66675017888697 36.0 30.0 38.0 4.0 40.0 53 30.399808111693797 35.0 30.0 38.0 4.0 40.0 54 30.226162480616622 35.0 30.0 38.0 4.0 40.0 55 29.878920566592456 35.0 30.0 38.0 4.0 40.0 56 17.629730540229165 20.0 15.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.7103762484818787 1 2 -0.8476600692166016 1 3 -0.9224485510033844 1 4 -1.0999547292440255 1 5 -0.8246671518588968 1 6 -1.0108475892979172 1 7 -1.2298790135840818 1 8 -1.4693723372208751 1 9 -1.4844498320571375 1 10 -0.4962815979755746 1 11 -0.45873246660630684 1 12 -0.6203437436678527 1 13 -0.7574568964337587 1 14 -0.6186182358527859 1 15 -0.5858145241072421 1 16 -0.6792837950504946 1 17 -0.6875008571273398 1 18 -0.7053290786446311 1 19 -0.8262830648334045 1 20 -0.8840379955160742 1 21 -0.5581900494164103 1 22 -0.7947657710173672 1 23 -0.5619738279515474 1 24 -0.6782458332722214 1 25 -0.7517947339451041 1 26 -0.545007125748981 1 27 -0.5452097243387612 1 28 -0.40669264130281846 1 29 -0.583970418062151 1 30 -0.4250292409629637 1 31 -0.3238190587971488 1 32 -0.3300954503347171 1 33 -0.4168604624123944 1 34 -0.09271283490900828 1 35 -0.24549540600877862 1 36 0.11760232634459555 1 37 0.30135348455399935 1 38 0.061472999351089186 1 39 0.15724856440196788 1 40 0.20653600639307257 1 41 0.4441531380652819 1 42 0.33915698841238395 1 43 0.44273285203096435 1 44 0.7062279028392737 1 45 0.7390160637373953 1 46 0.6427518897202518 1 47 0.9322448257110381 1 48 1.061602988955734 1 49 0.9674356760631753 1 50 1.0588814958662098 1 51 0.6777295239578116 1 52 1.1925625547635583 1 53 1.068860823774095 1 54 1.1981184732703518 1 55 1.3406494516903322 1 56 2.007848181787203 2 1 0.001924601500128631 2 2 0.37723124987761736 2 3 -0.12021535746584533 2 4 0.015757256033182898 2 5 0.18216632598907267 2 6 0.03650602327309116 2 7 0.3196010286495863 2 8 0.04994838499840171 2 9 0.07545590900802068 2 10 0.26602721505787486 2 11 -0.14580224063260516 2 12 -0.032770822761598595 2 13 -0.07754797014801795 2 14 -0.12397707454495333 2 15 -0.09174552279186088 2 16 -0.24155780659987158 2 17 -0.18259030873853277 2 18 -0.15075563276683823 2 19 -0.2607294558015312 2 20 -0.3513747382151351 2 21 -0.3695660057199035 2 22 -0.07211000488334207 2 23 -0.22370930128122524 2 24 -0.19328420338305818 2 25 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-0.3022436953789409 120 34 -0.2801128622339917 120 35 -0.28234315327365067 120 36 0.15804751346821888 120 37 0.42094234857013646 120 38 0.37880755179930503 120 39 0.1706073309940166 120 40 0.3951057534267335 120 41 0.36803728011318526 120 42 0.3619271116901217 120 43 0.5522566615547717 120 44 0.6310694704528572 120 45 0.339863138387166 120 46 0.34852166618082947 120 47 0.1478726173264704 120 48 0.3955490326126565 120 49 -0.13979703825406986 120 50 0.14447492105354343 120 51 0.3767142955314142 120 52 -0.002069135917569298 120 53 0.5326184301050851 120 54 0.3662081412717413 120 55 0.40067824802457963 120 56 -0.08413613787312713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 410.0 5 43.0 6 111.0 7 175.0 8 379.0 9 521.0 10 543.0 11 838.0 12 959.0 13 1269.0 14 1773.0 15 2227.0 16 2794.0 17 3287.0 18 4129.0 19 4880.0 20 4749.0 21 5940.0 22 6813.0 23 7563.0 24 10731.0 25 17633.0 26 12722.0 27 13070.0 28 15121.0 29 16388.0 30 19678.0 31 24432.0 32 29548.0 33 37894.0 34 51701.0 35 75557.0 36 118020.0 37 181186.0 38 229450.0 39 151204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.20632132450055 0.36716777582921334 0.18704952634692276 0.23946137332331938 8 98.85218147205472 0.980414486333415 0.07914680878928158 0.08825723282258019 9 94.51837173946464 3.61484543596226 0.9207222288652398 0.9460605957078514 10 30.320819786322595 54.41115343662276 6.769614458242941 8.498412318811697 11 35.19043633237104 23.335971560292975 26.10316796015708 15.370424147178902 12 33.04958158479622 17.001095148889004 24.78804029085029 25.161282975464488 13 25.26434464734118 18.915802599887257 30.27659626966096 25.543256483110603 14 21.121284417948296 23.49122836986044 31.071101165564873 24.31638604662639 15 24.840330328791406 25.958160377627078 27.958562754688547 21.24294653889297 16 31.30664358692578 26.0618863512562 23.866369059481578 18.765101002336444 17 29.39535254494001 23.871019171748575 25.34358635638081 21.390041926930603 18 27.281829069465086 24.353397144261667 25.833366548420955 22.531407237852292 19 26.29230415909837 25.980177235707547 25.225055943697576 22.5024626614965 20 25.37623204250013 27.280880066961615 25.790946136515906 21.551941754022348 21 24.706331175301642 24.098684872330693 30.2209847229577 20.97399922940997 22 24.197096431940388 21.641622490600128 31.024789843395606 23.136491234063875 23 22.59685784376527 24.463551082577688 30.06979933284934 22.8697917408077 24 23.699556910310637 26.085731069517347 26.694137151869963 23.520574868302052 25 24.045284767281338 27.720399602751872 24.976880994563473 23.257434635403314 26 22.254920673809682 27.097963358108824 25.603647285949382 25.043468682132115 27 21.066713031449193 24.016577309949522 27.56310313408828 27.353606524513 28 22.23507011019624 25.834642931117376 26.31312910575533 25.61715785293106 29 24.86743570905144 25.137839299478866 26.10045707236076 23.894267919108934 30 25.309815837113973 23.697451171958612 27.901357415269757 23.09137557565766 31 26.62858284420514 23.78780239538047 28.537562191421152 21.046052568993236 32 28.1989695668068 23.516895905684517 27.138358313531956 21.145776213976724 33 26.972021566114307 23.53037519782587 28.124410807434252 21.373192428625572 34 23.097942292764927 25.66281412060517 30.862846502945363 20.376397083684537 35 21.71962253582309 29.613847974073632 28.516149682493413 20.15037980760987 36 24.303496146310597 28.9199187332759 25.991724668948642 20.78486045146486 37 23.97934406839152 29.381620259737762 25.9169435375687 20.72209213430202 38 25.012016021361816 27.961377074194164 25.762349799732974 21.264257104711042 39 24.74906659927426 25.277206902635456 26.165737636910695 23.80798886117959 40 24.26476139687928 26.76437673040184 25.504486501312645 23.466375371406237 41 21.062827564883136 27.38776429452115 26.87253550965565 24.676872630940068 42 20.624182017608756 25.635031172825656 28.052679259259968 25.688107550305617 43 21.5325336741341 25.313817522584735 28.28655919216464 24.867089611116526 44 23.490858916867886 25.546969582595587 26.2814859264681 24.680685574068427 45 22.915141704428397 26.762442258479428 25.810196908857748 24.512219128234435 46 21.75824091785514 27.733724886267062 26.424660952058666 24.083373243819132 47 20.877140167533963 27.448714824318703 27.897797592595246 23.77634741555208 48 22.890608026691194 26.68066563365265 27.75815221292467 22.670574126731488 49 24.386260501669074 25.286000357829337 27.284486758879478 23.043252381622114 50 21.654681627873156 26.14467007018806 27.447110196021402 24.75353810591738 51 20.757738225624554 27.15981125415739 28.315798233640415 23.76665228657764 52 20.97036908018015 27.14690665906163 28.54414013677576 23.338584123982457 53 21.558798332559597 26.055909985019838 29.66435822209885 22.720933460321717 54 21.336215769080898 25.724302935316572 28.493317772265236 24.446163523337294 55 22.580206581975972 26.278498852960663 26.61006790319677 24.53122666186659 56 21.52622785222208 27.24929505316424 25.570635912313712 25.65384118229997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 7.5 5 15.0 6 31.5 7 48.0 8 71.0 9 94.0 10 497.0 11 900.0 12 1286.0 13 1998.5 14 2325.0 15 2441.0 16 2557.0 17 2969.5 18 3382.0 19 3890.5 20 4399.0 21 4712.5 22 5727.0 23 6428.0 24 7199.0 25 7970.0 26 9108.0 27 10246.0 28 12314.0 29 16150.0 30 17918.0 31 20283.0 32 22648.0 33 23524.5 34 24401.0 35 26452.0 36 28503.0 37 30388.5 38 35269.5 39 38265.0 40 39638.5 41 41012.0 42 41607.5 43 42203.0 44 44404.5 45 46606.0 46 49630.0 47 52695.5 48 52737.0 49 54831.0 50 56925.0 51 57055.5 52 57186.0 53 57180.5 54 56983.5 55 56792.0 56 57748.0 57 58704.0 58 60099.5 59 61495.0 60 66575.5 61 71656.0 62 68798.5 63 52419.0 64 38897.0 65 31825.5 66 24754.0 67 20572.0 68 16390.0 69 13923.5 70 11457.0 71 9128.0 72 5626.0 73 4453.0 74 3618.0 75 2783.0 76 2175.0 77 1567.0 78 1275.0 79 755.5 80 528.0 81 423.5 82 319.0 83 236.0 84 153.0 85 113.5 86 74.0 87 51.0 88 18.5 89 9.0 90 6.5 91 4.0 92 2.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.051341035437651485 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.8470075104058127E-4 24 9.490025034686042E-5 25 0.046690923170655325 26 0.06462707048621194 27 0.1153038041714354 28 0.11881511343426925 29 0.15250470230740468 30 0.24190073813414717 31 0.24180583788380033 32 0.3137402276467205 33 0.3389836942389854 34 0.3590076470621729 35 0.3792214003860542 36 0.41319569001023027 37 1.3137041655515886 38 0.4875974862821688 39 0.4890209900373717 40 0.7000791468087894 41 0.5555460655305209 42 0.5875274498974128 43 0.6445625003558759 44 0.6831869022470481 45 0.689355418519594 46 0.7257022144024415 47 0.7006485483108704 48 0.844517327836711 49 0.8111124397146161 50 0.8110175394642691 51 0.7887159806327568 52 0.8366406070579214 53 0.8562849588797214 54 0.8779222159588058 55 0.8835213307292705 56 0.8857040364872483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 1053738.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.207224953724065 #Duplication Level Percentage of deduplicated Percentage of total 1 59.069975349523105 18.43410008743503 2 12.9776536359496 8.099931127771885 3 7.544705342769624 7.063479505241266 4 4.93979865250929 6.166296310998417 5 3.5274296586509677 5.504064543297943 6 2.5602895563268144 4.793971927857676 7 1.8585169186858173 4.059940889321128 8 1.3847428136195294 3.4571184390141965 9 1.055056373135608 2.9632843417772845 >10 4.737714916326869 24.593968803566984 >50 0.23230392187216647 4.934317483938344 >100 0.10159174041784651 5.8231867315426555 >500 0.006814080141840774 1.5259465327928772 >1k 0.0030973091553821697 1.9433176057389496 >5k 3.09730915538217E-4 0.6370756697053179 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 6591 0.625487550036157 No Hit TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 4184 0.39706264745126396 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 3076 0.2919131700669426 No Hit TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 2289 0.21722667304396348 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 2089 0.19824662297459142 No Hit TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1860 0.17651446564516038 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 1749 0.16598053785665887 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA 1387 0.1316266472310954 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 1238 0.11748650992941319 No Hit TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1222 0.11596810592386343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.592020027748834E-4 0.0 0.0 0.0 0.0 8 0.0010439027538154645 0.0 0.0 0.0 0.0 9 0.001138803004162325 0.0 2.8470075104058127E-4 0.0 0.0 10 0.0023725062586715103 0.0 2.8470075104058127E-4 0.0 0.0 11 0.003416409012486975 0.0 2.8470075104058127E-4 0.0 0.0 12 0.003511309262833835 0.0 2.8470075104058127E-4 0.0 0.0 13 0.004839912767689881 0.0 2.8470075104058127E-4 0.0 0.0 14 0.006927718275320811 0.0 2.8470075104058127E-4 0.0 0.0 15 0.008256321780176856 0.0 2.8470075104058127E-4 0.0 0.0 16 0.008635922781564298 0.0 2.8470075104058127E-4 0.0 0.0 17 0.00920532428364546 0.0 2.8470075104058127E-4 0.0 0.0 18 0.009964526286420344 0.0 2.8470075104058127E-4 0.0 0.0 19 0.01110332929058267 0.0 2.8470075104058127E-4 0.0 0.0 20 0.012811533796826155 0.0 2.8470075104058127E-4 0.0 0.0 21 0.013191134798213598 0.0 2.8470075104058127E-4 9.490025034686042E-5 0.0 22 0.015468740806538248 0.0 2.8470075104058127E-4 9.490025034686042E-5 0.0 23 0.016987144812088014 0.0 2.8470075104058127E-4 9.490025034686042E-5 0.0 24 0.01860044906798464 0.0 2.8470075104058127E-4 9.490025034686042E-5 1.8980050069372084E-4 25 0.019454551321106385 0.0 2.8470075104058127E-4 9.490025034686042E-5 1.8980050069372084E-4 26 0.02059335432526871 0.0 2.8470075104058127E-4 9.490025034686042E-5 1.8980050069372084E-4 27 0.021637257079084173 0.0 2.8470075104058127E-4 9.490025034686042E-5 1.8980050069372084E-4 28 0.026856770848161498 0.0 2.8470075104058127E-4 1.8980050069372084E-4 1.8980050069372084E-4 29 0.028849676105445567 0.0 2.8470075104058127E-4 1.8980050069372084E-4 1.8980050069372084E-4 30 0.030937481613076495 0.0 2.8470075104058127E-4 1.8980050069372084E-4 1.8980050069372084E-4 31 0.0323609853682794 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 32 0.03378448912348231 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 33 0.035207992878685215 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 34 0.036916197384928705 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 35 0.04403371616094323 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 36 0.046026621418227304 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 37 0.04792462642516451 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 38 0.04972773118175486 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 39 0.05276453919285439 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 40 0.0546625441997916 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 41 0.05712995070880997 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 42 0.05902795571574718 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 43 0.06215966397719357 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 44 0.06481687098690567 0.0 2.8470075104058127E-4 4.745012517343021E-4 1.8980050069372084E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGATAG 25 2.1454975E-5 49.76828 6 CTACCGG 104550 0.0 49.15897 2 TCTACCG 105745 0.0 49.151737 1 TACCGGG 98750 0.0 49.138306 3 CGGGACT 3465 0.0 49.121937 6 ACCGGGA 54390 0.0 49.09116 4 TACCGGA 4005 0.0 49.022686 3 CCGGGAT 17490 0.0 48.957302 5 ACCGGGT 5800 0.0 48.91021 4 ACCGGGG 30245 0.0 48.90439 4 CGGGATA 1970 0.0 48.88407 6 CGGGATT 6070 0.0 48.82539 6 CCGGGAC 9075 0.0 48.671455 5 CGGGATG 4985 0.0 48.52033 6 CCGGGAG 17605 0.0 48.41135 5 CGGGATC 4590 0.0 48.304504 6 TACCGGT 920 0.0 48.145405 3 ACCGGGC 8740 0.0 48.003044 4 TACCGGC 970 0.0 47.97252 3 CCGGGGT 5565 0.0 47.934948 5 >>END_MODULE