FastQCFastQC Report
Fri 17 Jun 2016
SRR1526643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315928
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56771.7969284140690285No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.8242384340735863No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14250.451052138461928No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10350.3276062900407688No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.29437086931199513No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.24182725177888634No Hit
GTAATGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.19529766275860325No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6070.19213238459395812No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.18738446734699046No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT5640.17852168848598415No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.1769390494036616No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.17598946595426807No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4580.144969739940746No Hit
GTAATGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.14307057304195892No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4300.13610696107973969No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA4120.13040946038337847No Hit
GTAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC4100.12977640475044946No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.11711529209186905No Hit
GTAATGGGGGAAAAAGAATGTTCGGGACCAGTTTAATAGTCATATCCAGCTAGTGAGGAATGGA3640.11521612519308196No Hit
GTAATGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA3330.10540376288268212No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3320.10508723506621762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC150.00463822958.14426450
CCTATTC150.00464115158.13501747
CATCATA258.5526735E-658.11653543
ATCGCTC150.004646999358.1165341
TGAACTA201.9898554E-458.10729638
TGACATG150.004682203358.00586733
GACGTAT150.00468514657.99666628
TCACTTA150.00468514657.99666631
AGCTATA150.00468514657.99666628
TACTAAC258.715169E-657.9323325
CGATGCA258.731558E-657.9139723
CGATGTA202.0276716E-457.88646320
TCCTAGG150.004720564557.8864620
ATATGAC202.0324385E-457.8589812
CACAACT150.0047294557.85897414
ATGGGGG140900.056.2574844
AATGGGG305500.054.8953
ATGGGGC30100.054.4950834
TAATGGG329300.054.274632
GTAATGG335200.054.1219641
Produced by FastQC (version 0.11.5)