FastQCFastQC Report
Fri 17 Jun 2016
SRR1526642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307198
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43701.422535302964212No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.6621136856359743No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.3584007708383518No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8170.2659522522933092No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7480.24349116856229536No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.18945435842681269No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5150.1676443206010456No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.16276147631169474No Hit
GTAATGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780.15559997135398015No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4240.13802173191231715No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.13183679581247273No Hit
GTAATGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.12532633676000496No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.12337319904426461No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3730.12142006132852429No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.1210945383759009No Hit
GTAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3350.10905018912883548No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3090.10058659236062734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG150.004614958558.2177533
TTGACGG150.004614958558.2177534
GCTATTT150.00464200658.13189757
TATTCGG150.00464200658.13189755
TATAGAA150.00464200658.13189757
CCACGTA150.00464200658.13189758
AACGTAT201.9952723E-458.07480628
ACGATAC150.004660103558.07480645
CGATACA150.004660103558.07480646
TATACTA150.004669171858.046330
CTATCAG150.004672197658.03680425
CCTACTA258.680661E-657.97042523
GATATGA150.004693418357.9704223
CTATGCG150.004705576757.9325615
CCGATTA150.004705576757.9325614
TCCGATT150.004714710657.9041913
ATCCGAT150.004714710657.9041912
TAGTTAT150.004729963457.8569817
TACGCTT258.807561E-657.8286910
GGATCTA202.0441887E-457.791018
Produced by FastQC (version 0.11.5)