FastQCFastQC Report
Fri 17 Jun 2016
SRR1526641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277101
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40801.4723873244773567No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT26980.9736522062352716No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11840.42728102749538976No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA11520.415732891617136No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11220.40490651423127305No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA8660.31252142720524284No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.25045019685962877No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.2031750156080274No Hit
GTAATGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.18477017405206045No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.18260489857488788No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG4460.160952143803162No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA4170.15048664566349454No Hit
GTAATGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.1439908192319768No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.13966026827763162No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.1371341135542636No Hit
GTAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3690.13316444184611387No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3470.12522509842981439No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3140.11331608330536519No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3060.11042904933580173No Hit
GTAATGGGGGAAAAAGAATGTTCGGGACCAGTTTAATAGTCATATCCAGCTAGTGA3030.10934641159721546No Hit
GTAATGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3030.10934641159721546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA203.9175653E-450.6570435
CCTAATT150.00813507950.4436931
TATCTAG150.00813507950.4436931
AGATAAT150.00824755550.26879547
ATCATAC150.00825350750.25962444
TGCCTAA150.00825946250.25045450
TATACCC150.00825946250.25045450
TGATTTG150.0082654250.2412943
CATCATA150.0082654250.2412943
TCTATCA150.0082654250.2412943
ATCTAGG150.00827734550.22297338
AATCTAC150.00834316650.1224541
ACGCTTG204.1281746E-450.1224541
CGGAATG204.202848E-449.9407230
CATGCGG252.0979809E-549.9407229
TAGGTCT150.00846383549.9407229
CCATGCG252.1024858E-549.9226228
GACGTAT150.00847597349.92261528
CAATATC150.00847597349.92261528
AATAGGT150.00850637449.87742227
Produced by FastQC (version 0.11.5)