FastQCFastQC Report
Fri 17 Jun 2016
SRR1526640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47890
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.8352474420547087No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.7642514094800584No Hit
CCTAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.6285237001461683No Hit
CCTAGAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA2910.6076425140948005No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2040.4259761954479014No Hit
CCTAGAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC1400.2923366047191481No Hit
CCTAGAGGGAAGATTTATTTTTTAAAAATTATTTTTGTGGACATGGGGATCCCGTGACGGCACC1320.2756316558780539No Hit
CCTAGAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT1290.2693673000626436No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.2672791814575068No Hit
CCTAGAGGGACATAAGGTAGATTCAGAAGGAATCTCACCAAACCGTACCAGTCACGCGGCACCT1220.25475046982668614No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.20254750469826688No Hit
CCTAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG970.20254750469826688No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA920.192106911672583No Hit
CCTAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG880.1837544372520359No Hit
CCTAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.16704948841094175No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG780.1628732512006682No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.15869701399039465No Hit
CCTAGAGGGTGAGTTTGATTATTTCCTGCTGTCTGTTCCTCTTGGGTATATTCAGTTCTTATTG750.15660889538525788No Hit
CCTAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG710.14825642096471078No Hit
CCTAGAGGGGCAAGTTGAATTTTGTCCCTTAGCATTTATTTCTGTTTTATTTCTTTTAATTAAG700.14616830235957404No Hit
CCTAGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG700.14616830235957404No Hit
CCTAGAGGGGAGTCAGTGTCTGCTGTGGTCTCTTCGGTTTGTTGCCACCATGGGGAAGCGACAG700.14616830235957404No Hit
CCTAGAGGGGGGGTTTCTGATCCTTCTGCCAAGATGCTCAATCCTTGTCTCCCACTCTCATGCC680.1419920651493005No Hit
CCTAGAGGGGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTCAAGGGGAG660.13781582793902694No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1336395907287534No Hit
CCTAGAGGGCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCTATCTGTCCTCTA630.13155147212361662No Hit
CCTAGAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACTTGTGCAGGACCA620.12946335351847985No Hit
CCTAGAGGGGGCAGGTACCACCTGACTGGTCAATGCCTCCTGACTGGTCCTTTCCCTCCGACTG620.12946335351847985No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC610.12737523491334307No Hit
CCTAGAGGGACTGTACACACACACACACACACACACAAACACTATAGAAAATTTACATTGTTTT600.1252871163082063No Hit
CCTAGAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG590.12319899770306952No Hit
CCTAGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11902276049279598No Hit
CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11902276049279598No Hit
CCTAGAGGGGGAGAAAAATCCCTCCTTTGTTCCTGTGGTAGAAGCCACTGCGTCTGTCCCCTGT530.1106702860722489No Hit
CCTAGAGGGGTGTCTGGGAGGAGTTCCCTATGCCTCCCTGGTGGCCACCATCCTATGTTTCTCT530.1106702860722489No Hit
CCTAGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCCGTATCA510.10649404886197535No Hit
CCTAGAGGGGAGCATACCCAGGGCTTCATAATCACCTTCTGTTCAGCACTCGATAACATTCTTG510.10649404886197535No Hit
CCTAGAGGGGAGTATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTGGCCCTGTC510.10649404886197535No Hit
CCTAGAGGGGTAGTGGAAACTAGACTGACAGCTGGCCTAAGAATTGCTGCTGAAGAGACCCGAG490.10231781165170181No Hit
CCTAGAGGGACTCCAGCACTGGGTTATGGACACCCATTCTCGTGTACAGCTTTTTGCGTGGGTG490.10231781165170181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT201.9649518E-458.15336249
ATCCTGG201.9649518E-458.15336253
CCGTGAC201.9649518E-458.15336252
GTGCAAT150.00461127258.15336252
ATGACCC150.00461127258.15336249
GTTCTTA150.00461127258.15336255
CACGCGG201.9649518E-458.15336253
TAATGAC150.00461127258.15336247
GCAATGA150.00461127258.15336254
TCAGTGC150.00461127258.15336249
TCACGCG201.9649518E-458.15336252
CGCGGCA201.9649518E-458.15336255
CGTGACG201.9649518E-458.15336253
ACGCGGC201.9649518E-458.15336254
AGACACG501.8189894E-1258.15336255
TGACGGC201.9649518E-458.15336255
AATGAGC150.00461127258.15336256
ACCAGTC201.9649518E-458.15336247
AATGACC150.00461127258.15336248
AAACCGT201.9751968E-458.09234240
Produced by FastQC (version 0.11.5)