FastQCFastQC Report
Fri 17 Jun 2016
SRR1526639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46756
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.793481050560356No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.5496620754555566No Hit
CCTAGAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA2260.48336042433056714No Hit
CCTAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.4534177431773462No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1790.38283856617332535No Hit
CCTAGAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC1080.23098639746770466No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.23098639746770466No Hit
CCTAGAGGGACATAAGGTAGATTCAGAAGGAATCTCACCAAACCGTACCAGTCACGCGGCACCT1070.22884763452818888No Hit
CCTAGAGGGAAGATTTATTTTTTAAAAATTATTTTTGTGGACATGGGGATCCCGTGACGGCACC1020.21815381983060997No Hit
CCTAGAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT920.19676619043545213No Hit
CCTAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG870.18607237573787322No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.18393361279835743No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA800.17110103516126274No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG710.15185216870562068No Hit
CCTAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1497134057661049No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.14543587988707332No Hit
CCTAGAGGGCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCTATCTGTCCTCTA630.13474206518949441No Hit
CCTAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG630.13474206518949441No Hit
CCTAGAGGGGGGGTTTCTGATCCTTCTGCCAAGATGCTCAATCCTTGTCTCCCACTCTCATGCC610.13046453931046284No Hit
CCTAGAGGGGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTCAAGGGGAG580.12404825049191547No Hit
CCTAGAGGGGCAAGTTGAATTTTGTCCCTTAGCATTTATTTCTGTTTTATTTCTTTTAATTAAG580.12404825049191547No Hit
CCTAGAGGGACTCCAGCACTGGGTTATGGACACCCATTCTCGTGTACAGCTTTTTGCGTGGGTG570.12190948755239968No Hit
CCTAGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG570.12190948755239968No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1197707246128839No Hit
CCTAGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG540.11549319873385233No Hit
CCTAGAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACTTGTGCAGGACCA540.11549319873385233No Hit
CCTAGAGGGGGAGAAAAATCCCTCCTTTGTTCCTGTGGTAGAAGCCACTGCGTCTGTCCCCTGT530.11335443579433654No Hit
CCTAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG520.11121567285482077No Hit
CCTAGAGGGGAGTCAGTGTCTGCTGTGGTCTCTTCGGTTTGTTGCCACCATGGGGAAGCGACAG520.11121567285482077No Hit
CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.11121567285482077No Hit
CCTAGAGGGGGCAGGTACCACCTGACTGGTCAATGCCTCCTGACTGGTCCTTTCCCTCCGACTG510.10907690991530498No Hit
CCTAGAGGGGAGTATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTGGCCCTGTC500.1069381469757892No Hit
CCTAGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCCGTATCA500.1069381469757892No Hit
CCTAGAGGGACTGTACACACACACACACACACACACAAACACTATAGAAAATTTACATTGTTTT470.10052185815724185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAC150.004522869458.43553536
TTCTCGT150.004522869458.43553537
TTGATGC150.004522869458.43553537
ATTCTCG150.004522869458.43553536
GCTCAAT150.004522869458.43553536
CTCTACG201.9178768E-458.4355337
ATTTATC201.9178768E-458.4355336
TGGTTGA150.00454233258.37229534
CAATCCT150.00454233258.37229539
TAAGAAT258.221412E-658.37229538
TCAATCC150.00454233258.37229538
CTGGTTG150.00454233258.37229533
TCTCGTG150.00454233258.37229538
GGCCTAA258.221412E-658.37229534
TGGACAT150.00454233258.37229538
CTACGTA150.00454233258.37229539
TAGAGCG150.00454233258.37229534
AAGCCCA150.00454233258.37229534
TCTACGT201.9281925E-458.3722938
CATTCTC150.004561857358.30919335
Produced by FastQC (version 0.11.5)