SRR1526638_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43084
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	433	1.0050134620740878	No Hit
CCTAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	338	0.7845139727044842	No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.6754247516479436	No Hit
CCTAGAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG	231	0.5361619162566149	No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	169	0.3922569863522421	No Hit
CCTAGAGGGACATAAGGTAGATTCAGAAGGAATCTCACCAAACCGTACCAGTCACG	160	0.37136756104354285	No Hit
CCTAGAGGGAAGATTTATTTTTTAAAAATTATTTTTGTGGACATGGGGATCCCGTG	152	0.3527991829913657	No Hit
CCTAGAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG	140	0.3249466159131	No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	118	0.27388357626961285	No Hit
CCTAGAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT	115	0.26692043450004643	No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.24603100919134716	No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.23906786742178074	No Hit
CCTAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	100	0.23210472565221427	No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.16479435521307215	No Hit
CCTAGAGGGGTGGTTGGAGGCTGTGAACGAGTTGGAGGATTTAATCTTCCATACAC	69	0.16015226070002786	No Hit
CCTAGAGGGGAGCATACCCAGGGCTTCATAATCACCTTCTGTTCAGCACTCGATAA	68	0.15783121344350573	No Hit
CCTAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	67	0.15551016618698357	No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	66	0.15318911893046142	No Hit
CCTAGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	66	0.15318911893046142	No Hit
CCTAGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	64	0.14854702441741713	No Hit
CCTAGAGGGGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTC	63	0.146225977160895	No Hit
CCTAGAGGGGGGGTTTCTGATCCTTCTGCCAAGATGCTCAATCCTTGTCTCCCACT	61	0.1415838826478507	No Hit
CCTAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	61	0.1415838826478507	No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	61	0.1415838826478507	No Hit
CCTAGAGGGGTGTCTGGGAGGAGTTCCCTATGCCTCCCTGGTGGCCACCATCCTAT	59	0.13694178813480642	No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	59	0.13694178813480642	No Hit
CCTAGAGGGTGAGTTTGATTATTTCCTGCTGTCTGTTCCTCTTGGGTATATTCAGT	57	0.13229969362176214	No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	57	0.13229969362176214	No Hit
CCTAGAGGGGGAGAAAAATCCCTCCTTTGTTCCTGTGGTAGAAGCCACTGCGTCTG	55	0.12765759910871785	No Hit
CCTAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.12765759910871785	No Hit
CCTAGAGGGCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCTATCT	54	0.12533655185219572	No Hit
CCTAGAGGGGAGTCAGTGTCTGCTGTGGTCTCTTCGGTTTGTTGCCACCATGGGGA	53	0.12301550459567358	No Hit
CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.12301550459567358	No Hit
CCTAGAGGGGCAAGTTGAATTTTGTCCCTTAGCATTTATTTCTGTTTTATTTCTTT	53	0.12301550459567358	No Hit
CCTAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	52	0.12069445733915143	No Hit
CCTAGAGGGGCCCAAGGCCCATGGACATTGATTCACCTTCAGCGGGACCCTTTCCC	50	0.11605236282610713	No Hit
CCTAGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.11605236282610713	No Hit
CCTAGAGGGACTCCAGCACTGGGTTATGGACACCCATTCTCGTGTACAGCTTTTTG	48	0.11141026831306285	No Hit
CCTAGAGGGGGCTGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGA	47	0.10908922105654072	No Hit
CCTAGAGGGGACTTTAAAGTCTCCAGGCTTTGCCTGGGTTTGAGTATGTGCAAAGT	47	0.10908922105654072	No Hit
CCTAGAGGGGGCAGGTACCACCTGACTGGTCAATGCCTCCTGACTGGTCCTTTCCC	47	0.10908922105654072	No Hit
CCTAGAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	46	0.10676817380001857	No Hit
CCTAGAGGGAAGATTTGTGACGAGCTGATCGCCAAGATGGGAAAAAGCTAACTCTG	46	0.10676817380001857	No Hit
CCTAGAGGGCAGACCTACAGGGTCAGATCTCACGGAGTGTAGTTTAGATTTCAGCT	45	0.10444712654349643	No Hit
CCTAGAGGGGACCGGGACGATCATCCTGGGGACCTGGTACATGGTGGTCAACCTGT	44	0.10212607928697429	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTGG	20	3.9083595E-4	50.582058	34
AAAGGCT	25	1.9174098E-5	50.582054	35
TAGGATT	15	0.008000883	50.58205	34
CAGTACG	15	0.008000883	50.58205	37
ACCAAAC	30	9.4538336E-7	50.58205	37
CACCAAA	30	9.4538336E-7	50.58205	36
TCACCAA	30	9.4538336E-7	50.58205	35
TTTGTGG	15	0.008000883	50.58205	34
GGTAGGA	20	3.9312354E-4	50.522408	32
GTAGGAT	20	3.9312354E-4	50.522408	33
CGGTAGG	20	3.9312354E-4	50.522408	31
AATCTCA	30	9.531359E-7	50.522404	31
CGTTAAA	25	1.9308804E-5	50.522404	31
TAGCCGA	15	0.008038352	50.522404	31
CGGGTAG	25	1.9308804E-5	50.522404	31
GTTAAAG	25	1.9308804E-5	50.522404	32
GGTAAAG	15	0.008038352	50.522404	32
TCCCGTG	15	0.008227661	50.226257	50
CCAGGTG	15	0.008227661	50.226257	50
CAACCAA	20	4.0707356E-4	50.16745	49

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content