FastQCFastQC Report
Fri 17 Jun 2016
SRR1526637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206713
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13620.6588845404014262No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8060.38991258411420665No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7970.38555872151243514No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7290.3526628707434946No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.2955788944091567No Hit
CTTCTGGGGGGAAGGTCCCTACCGCTCAGTGCTAGAAAGTTGTGTATGTGTGTCTGTTTGTCTG5340.2583291810384446No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4940.23897868058612667No Hit
CTTCTGGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4750.22978719287127564No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4540.2196281801338087No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA4020.19447252954579536No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT3980.19253747950056357No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3700.17899212918394103No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3680.17802460416132512No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3600.17415450407086153No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3290.1591578662203151No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCTCCGACTGTTCTTCCAAAGGTCCT3240.15673905366377539No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3220.15577152864115948No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT3120.15093390352808No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG3100.1499663785054641No Hit
CTTCTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.14754756594892435No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAAGGTCCGAAGTTCAAATC3020.1460962784150005No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA3000.1451287533923846No Hit
CTTCTGGGGGGCCATCTCTGCAGATTTTGTGAATCTCCGGTTTGGCTCCCTGTCTTCCCAGCCA2890.13980736576799718No Hit
CTTCTGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2810.1359372656775336No Hit
CTTCTGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG2720.13158340307576205No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2680.12964835303053024No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2570.12432696540614283No Hit
CTTCTGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT2480.11997310280437129No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2470.11948934029306334No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2450.11852181527044743No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTGTCCGGCAGCCT2400.1161030027139077No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1141679526686759No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC2350.11368419015736794No Hit
CTTCTGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT2330.11271666513475204No Hit
CTTCTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2330.11271666513475204No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA2330.11271666513475204No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2300.1112653776008282No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG2300.1112653776008282No Hit
CTTCTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGCC2250.10884656504428845No Hit
CTTCTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG2240.1083628025329805No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2220.10739527751036462No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2180.10546022746513281No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC2150.10400893993120898No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2070.1001388398407454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGA201.9691902E-458.22046358
ACGAGCT150.00461196658.22046357
GCTACTA150.004616417558.2062855
GACGAGC150.004616417558.2062856
GCGTACT150.004616417558.2062853
ATGCGTA150.004620871558.19211251
CATGCGT201.9739392E-458.1921150
ATTCGTA201.9739392E-458.1921150
TTCGTAT201.9739392E-458.1921151
TCATGCG150.0046297958.16378849
AAATTCG201.9786974E-458.16378448
TAGACAA150.004638720358.1354944
CTAGTCC150.004647664358.10722435
TCGTTCA201.9954231E-458.0648731
TTATGCA201.9954231E-458.0648732
TCTAGTC150.004661103758.0648734
CGTTACA150.004661103758.0648733
CGCTACG150.004661103758.0648733
AATCTTC303.7176142E-758.0648733
GCCACTA150.004661103758.0648732
Produced by FastQC (version 0.11.5)