FastQCFastQC Report
Fri 17 Jun 2016
SRR1526636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200442
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC11060.5517805649514573No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC6610.32977120563554546No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6310.31480428253559634No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT5920.2953472825056625No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.24745312858582533No Hit
CTTCTGGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4810.23996966703585076No Hit
CTTCTGGGGGGAAGGTCCCTACCGCTCAGTGCTAGAAAGTTGTGTATGTGTGTCTGTTTGTCTG4480.22350605162590673No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4090.20404905159597292No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3960.19756338491932832No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3450.17211961564941478No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3330.16613284640943513No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT3330.16613284640943513No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3240.16164276947945042No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3100.15465820536614083No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2920.14567805150617136No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2870.1431835643228465No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCTCCGACTGTTCTTCCAAAGGTCCT2660.13270671815288215No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2570.1282166412228974No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2520.12572215403957254No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA2500.1247243591662426No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT2450.12222987198291775No Hit
CTTCTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.11574420530627313No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2320.11574420530627313No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAAGGTCCGAAGTTCAAATC2260.11275082068628332No Hit
CTTCTGGGGGGCCATCTCTGCAGATTTTGTGAATCTCCGGTTTGGCTCCCTGTCTTCCCAGCCA2210.11025633350295845No Hit
CTTCTGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG2130.10626515400963871No Hit
CTTCTGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT2120.10576625657297374No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2110.10526735913630875No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2100.10476846169964378No Hit
CTTCTGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT2070.10327176938964887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGT150.004577947358.32871633
CGATTAA201.9559E-458.2993235
AGCGAAT150.004587094858.2993239
TCGCCTA150.00463303758.15280557
GTCGAGG150.00463303758.15280555
GCTAACT150.004642266358.1235952
GTATTAG150.004651509758.09440232
CAAATTG258.560506E-658.09440231
ACCCGAA550.058.07982341
CGAATCT150.00465613658.0798241
CGCTTAG303.7103928E-758.0798249
ATAATTG150.00466076658.06524745
ATTAAGC150.00466076658.06524751
TTCTTAG150.004688618757.9779528
ACCTAAA258.727531E-657.90540711
TGCCTTA202.0225873E-457.90540713
TCGATAC453.274181E-1157.90540325
CGATACT453.274181E-1157.90540326
CTCGATA453.274181E-1157.8909224
TATCGAT258.792487E-657.83304610
Produced by FastQC (version 0.11.5)