FastQCFastQC Report
Fri 17 Jun 2016
SRR1526635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189837
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20111.0593298461311544No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9780.5151788112960066No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC7190.3787459768116858No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6890.3629429457903359No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG6180.32554243903980784No Hit
CTTCTGGGGGGAAGGTCCCTACCGCTCAGTGCTAGAAAGTTGTGTATGTGTGTCTG5120.26970506276437156No Hit
CTTCTGGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4340.2286171821088618No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT4310.2270368790067268No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3940.20754647408039528No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA3690.1943772815626037No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3640.1917434430590454No Hit
CTTCTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.1743601089355605No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3280.17277980583342553No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3250.17119950273129053No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG3060.16119091641776895No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3020.15908384561492228No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2920.15381616860780564No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2880.151709097804959No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2830.14907525930140067No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAAGGTCCGAAG2610.1374863698857441No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2610.1374863698857441No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2610.1374863698857441No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCTCCGACTGTTCTTCCA2600.13695960218503242No Hit
CTTCTGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC2550.13432576368147411No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT2520.13274546057933911No Hit
CTTCTGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2490.13116515747720411No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA2440.1285313189736458No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2390.12589748047008748No Hit
CTTCTGGGGGGCCATCTCTGCAGATTTTGTGAATCTCCGGTTTGGCTCCCTGTCTT2370.12484394506866417No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.12062980346297086No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC2250.11852273266012422No Hit
CTTCTGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2250.11852273266012422No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2250.11852273266012422No Hit
CTTCTGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTC2240.11799596495941254No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2210.11641566185727756No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2150.11325505565300759No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2110.11114798485016093No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA2040.10746061094517928No Hit
CTTCTGGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2040.10746061094517928No Hit
CTTCTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1990.10482677244162096No Hit
CTTCTGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1940.10219293393806264No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTGTCC1910.10061263083592767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG251.9419269E-550.5868837
TATGAAT150.00806605150.54628434
GTTAGAA203.9569018E-450.5462834
GTTACTA203.9569018E-450.5462834
AGACACG150.00813452750.4383432
CGACTCA251.9760268E-550.43833532
TGTCGTA150.00814311750.42487731
TGATTAC150.00829889150.183847
TACGTAG252.0360949E-550.183840
TACGTAC301.0171079E-650.183840
TACTGAT150.0083076150.17047544
AGCTACC150.0083076150.17047545
GTATAAT204.1053942E-450.1704743
ATAGCCC150.00831633550.15715839
GCTGAAA150.00831633550.15715842
CTACGTA301.0208423E-650.15715839
AGGATTG150.00831633550.15715839
TGTACTG150.00831633550.15715842
GTACGTA252.0425048E-550.15715439
CTCGCAA150.00832506750.1438549
Produced by FastQC (version 0.11.5)