FastQCFastQC Report
Fri 17 Jun 2016
SRR1526634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286367
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.28913946090157033No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC7750.2706317417858901No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7290.25456843840246957No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC7180.2507272136803472No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6740.235362314791858No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA6240.21790220241857478No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6170.2154577866863151No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6140.21441017994391814No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4930.1721567080005727No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4620.1613314383291371No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.1382840899964032No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.1344428652742809No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3800.13269685403695258No Hit
GTTGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA3540.1236175956028453No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3530.12326839335537963No Hit
GTTGCCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACG3490.12187158436551698No Hit
GTTGCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3330.11628434840606636No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3160.11034791019915004No Hit
GTTGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA3160.11034791019915004No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3100.10825269671435604No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3040.10615748322956207No Hit
GTTGCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC3000.10476067423969941No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC2950.10301466300237107No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2910.10161785401250842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTCG150.00462950658.170556
TGCGCTA150.004632725458.1602953
ACGTTGT201.9840316E-458.13987451
CCTGTCG150.0046488558.1092846
GTAGTAA150.004652079758.0990945
ATCTAGT150.004658544458.07871636
ACGTTAT150.004658544458.07871638
CACGTTA150.004658544458.07871637
CGTTAAA150.00466825458.0481931
CCGGATA202.0344621E-457.845475
CGATTCA150.004733370657.8454716
TAGGGTA202.0344621E-457.8454713
CGTAATC150.004733370657.8454712
GCCGGAT1500.055.9172864
GTTGCCG299150.054.7322771
CGGGATT7400.054.718696
TTGCCGG295800.054.716572
TGCCGGG285450.054.4612733
CCGGGAT23450.054.268675
GCCGGGA87050.053.9913184
Produced by FastQC (version 0.11.5)