FastQCFastQC Report
Fri 17 Jun 2016
SRR1526633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278376
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.25002155358220535No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6230.22379802856568096No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5930.2130212374629997No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT5910.21230278472282094No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5480.1968560508089778No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4910.17638014771388338No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4880.17530246860361526No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA4700.1688363939420065No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4230.15195275454780585No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3920.1408167370750352No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3800.1365060206339627No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3390.12177773946029831No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.11710779664913641No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.11567089116877893No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3100.11136017472770642No Hit
GTTGCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2810.10094260999511451No Hit
GTTGCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC2800.10058338362502514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGA258.400664E-658.28944834
GACGGTA150.00459866458.26831436
CCCACGT201.9712097E-458.21553835
CGTATCT201.9729759E-458.20499830
AACTATC150.00465837658.0787945
TCGTAGC150.0046617158.06829554
CATTAGT201.9996181E-458.0473240
CGAACAG150.00471193657.91135414
CGTTGCA150.00471193657.91135415
TCTAGTA150.00471193657.91135414
AGTAGCG202.0301576E-457.86964416
GACGCAC150.004752406457.78640710
GATCACT202.0464753E-457.776029
CCGGATC303.8576945E-757.7656365
CGGGATA3300.056.8904046
CGGGTAT1250.055.455016
CCGGGAT24350.055.0374875
TTGCCGG286000.054.897552
GTTGCCG289800.054.875671
TGCCGGG275700.054.664693
Produced by FastQC (version 0.11.5)