FastQCFastQC Report
Fri 17 Jun 2016
SRR1526632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258770
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14900.5758009042779302No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8990.3474127603663485No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8880.34316188120724966No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG7360.28442246009970246No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT7220.2790122502608494No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT6080.23495768443018897No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5150.19901843335780808No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4750.18356069096108513No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4270.16501140008501758No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3970.15341809328747535No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.14955365768829462No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3620.13989256869034278No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG3090.11941106001468486No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2950.11400085017583181No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2910.11245507593615953No Hit
GTTGCCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2900.11206863237624146No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.10974997101673302No Hit
GTTGCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2740.10588553541755227No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2680.10356687405804381No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2670.10318043049812575No Hit
GTTGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2650.10240754337828961No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2600.10047532557869922No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG2590.10008888201878116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG354.7477442E-850.66377637
TCGCTTA203.9259283E-450.63387735
CGCTTAT203.9259283E-450.63387736
ACCGTTG150.00802057150.6239234
AGCTTAA150.008095683550.50470733
CTTGATC251.9627269E-550.50470733
TAGCTTA150.00810196650.494832
TAAGACA150.00812083750.46509631
TTATACC204.1123532E-450.16020648
GCGTTAT150.00831775150.16020248
GTCGTAG150.00831775150.16020248
ATTATCC150.00832416250.1504345
CGCGTTA150.00832416250.1504347
AATTGGG204.124234E-450.13089850
CGTGAGT150.00833699450.13089450
ACTACTA150.00833699450.13089450
TATACCG204.1282002E-450.1211349
CATTAGC150.00844666449.96544629
TACGACA252.0914586E-549.96544629
TTACGAC252.0962683E-549.94605328
Produced by FastQC (version 0.11.5)