FastQCFastQC Report
Fri 17 Jun 2016
SRR1526631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103794
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.7880995047883307No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5620.5414571169817137No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.3458774110256855No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.3362429427519895No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2800.26976511166348727No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2570.24760583463398653No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2510.24182515366976895No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1820.17534732258126673No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1710.16474940748020117No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1660.1599321733433532No Hit
CACAAGGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAGTCTGTGCTGCATGT1650.1589687265159836No Hit
CACAAGGGGAGGCTGTTCCCGTTATGTAAGTCAGTTCTTCAGGCTCATTTGCTGTGCTGGTTTT1600.1541514923791356No Hit
CACAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1580.15222459872439642No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1560.1502977050696572No Hit
CACAAGGGGAAAGTATATGACCATGAGATTGCTTCAGTATTTAGTTCTTTCTAAAAGGATGAAT1560.1502977050696572No Hit
CACAAGGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAG1490.14355357727807003No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1470.14162668362333083No Hit
CACAAGGGGCAACCATCTGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAAATCTTTTT1440.13873634314122205No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1440.13873634314122205No Hit
CACAAGGGGGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGACACTGTGAAAGGC1430.13777289631385245No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1410.13584600265911323No Hit
CACAAGGGGAAGATAATAGGCTTGTCAAAACTAAATAACGTACACTTTTTTTTTCAAGCTCTTG1380.13295566217700444No Hit
CACAAGGGGGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTG1260.12139430024856927No Hit
CACAAGGGGAGACTTGTATATCCATATCTCCATGGCAGTGAGAAACGGTAGCTTAGAGGTAATC1210.11657706611172128No Hit
CACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.11561361928435168No Hit
CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1160.11175983197487331No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1140.10983293832013412No Hit
CACAAGGGGGGTATTAGGTTACATGGTAGAATTTATTGGTATTATTGAAACCCTTGTGAAATAT1120.10790604466539493No Hit
CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1100.10597915101065573No Hit
CACAAGGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC1100.10597915101065573No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG1090.10501570418328612No Hit
CACAAGGGGAGGAGGGGCCCATCCCTCTCCCAGGCATTCATCAGCCTGCTGCCTTCAGCCTAAG1080.10405225735591653No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1060.10212536370117734No Hit
CACAAGGGGAACTTTGCTTGCGGTAGATTTTCTTTAAAAGAATGGGCAGTTACATGCATACTTC1050.10116191687380774No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1040.10019847004643814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGG150.004645077558.0947858
ATGGCTA150.004645077558.0947842
TGACGGT150.004645077558.0947842
CCGTCAT303.686273E-758.0947839
TGCATAC150.004645077558.0947855
TATTCTA150.004645077558.0947841
GAGCGAT303.686273E-758.0947858
TTTGGCA150.004645077558.0947856
CATACTT150.004645077558.0947857
AACCTGA150.004645077558.0947847
CTTTAGA303.686273E-758.0947853
CACCGCA150.004645077558.0947841
TCAAGCC150.004645077558.0947844
TGGCTAA150.004645077558.0947843
CCCGCCT150.004645077558.0947847
GCATACT150.004645077558.0947856
TACCCAA201.9856982E-458.09477655
GTATTAT201.9856982E-458.09477639
ACGCACA258.531211E-658.09477643
TACGCAC258.531211E-658.09477642
Produced by FastQC (version 0.11.5)