FastQCFastQC Report
Fri 17 Jun 2016
SRR1526630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101117
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.6734772590167826No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4820.4766755342820693No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.2927302036255031No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.24723834765667496No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2450.24229358070354143No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2280.22548137306288757No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1720.17009998318779238No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1440.14240928825024476No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1370.13548661451585786No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1350.13350870773460446No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1350.13350870773460446No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1330.13153080095335107No Hit
CACAAGGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAGTCTGTGCTGCATGT1310.12955289417209767No Hit
CACAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1260.12460812721896417No Hit
CACAAGGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAG1260.12460812721896417No Hit
CACAAGGGGAGGCTGTTCCCGTTATGTAAGTCAGTTCTTCAGGCTCATTTGCTGTGCTGGTTTT1240.12263022043771078No Hit
CACAAGGGGGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTG1200.11867440687520396No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1190.11768545348457726No Hit
CACAAGGGGAAAGTATATGACCATGAGATTGCTTCAGTATTTAGTTCTTTCTAAAAGGATGAAT1180.11669650009395056No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1160.11471859331269718No Hit
CACAAGGGGGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGACACTGTGAAAGGC1140.11274068653144378No Hit
CACAAGGGGCAACCATCTGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAAATCTTTTT1120.11076277975019039No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1110.10977382635956366No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1070.10581801279705688No Hit
CACAAGGGGAGACTTGTATATCCATATCTCCATGGCAGTGAGAAACGGTAGCTTAGAGGTAATC1040.10285115262517679No Hit
CACAAGGGGCTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCC1030.10186219923455009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC201.957535E-458.2611137
TCATATG150.004601469358.23203736
AGGTCGT150.004601469358.23203739
TAACGTA201.9623752E-458.23203336
GAAATCG258.46076E-658.17397757
TAAAATT150.0046196958.17397735
ATATGCT750.058.17397734
CCCAAAC150.0046196958.17397757
ATTTTTG150.0046196958.17397755
CTCATAT150.0046196958.17397735
TAGGGGT150.0046196958.17397755
ACTCATA150.0046196958.17397734
AGATGCA150.0046196958.17397756
TCTACTG201.9720844E-458.17397358
ATAACGT201.9720844E-458.17397335
AATAACG201.9720844E-458.17397334
CCGATGA150.004628820358.1449949
ACGGAGT150.004628820358.1449949
TCACTGC258.510939E-658.11603547
TGATAAT201.9818319E-458.1160341
Produced by FastQC (version 0.11.5)