FastQCFastQC Report
Fri 17 Jun 2016
SRR1526629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93697
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9210.9829556976210552No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5410.5773930862247457No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.43544617223603743No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.36500635025667844No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2940.3137773888171446No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2320.24760664695774678No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2000.21345400599805756No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1850.19744495554820324No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1780.18997406533827124No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1610.17183047482843633No Hit
CACAAGGGGGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTG1560.1664941246784849No Hit
CACAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1540.16435958461850433No Hit
CACAAGGGGGAGGATCGTCCAGAGTTATACTACGCAGGCGCCCGCTCTCTGCGCTC1450.15475415434859174No Hit
CACAAGGGGAGGCTGTTCCCGTTATGTAAGTCAGTTCTTCAGGCTCATTTGCTGTG1420.15155234425862088No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1410.15048507422863058No Hit
CACAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1390.14835053416865002No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1390.14835053416865002No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1330.1419469139887083No Hit
CACAAGGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCC1290.13767783386874713No Hit
CACAAGGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAGTCTGTG1280.13661056383875683No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1220.1302069436588151No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1210.12913967362882484No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1200.12807240359883454No Hit
CACAAGGGGAAAGTATATGACCATGAGATTGCTTCAGTATTTAGTTCTTTCTAAAA1190.12700513356884424No Hit
CACAAGGGGGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGACACTG1110.11846697332892195No Hit
CACAAGGGGCAACCATCTGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAA1090.11633243326894137No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1080.1152651632389511No Hit
CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1080.1152651632389511No Hit
CACAAGGGGAGACTTGTATATCCATATCTCCATGGCAGTGAGAAACGGTAGCTTAG1080.1152651632389511No Hit
CACAAGGGGAGGAGGGGCCCATCCCTCTCCCAGGCATTCATCAGCCTGCTGCCTTC1070.1141978932089608No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1050.11206335314898022No Hit
CACAAGGGGCTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCT1040.11099608311898994No Hit
CACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10779427302901906No Hit
CACAAGGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA980.10459246293904821No Hit
CACAAGGGGAACTTTGCTTGCGGTAGATTTTCTTTAAAAGAATGGGCAGTTACATG950.10139065284907735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCTA451.1823431E-1050.60304636
CGAACTG150.00801745450.60304334
GAGCTCA203.924534E-450.60304337
CCGTCGC203.924534E-450.60304336
GCCGTCG203.924534E-450.60304335
CTGCCGT203.966881E-450.49321733
GCGAACT150.00808666250.49321433
AAAGGAT150.00808666250.49321433
GGGTAGT150.00810403350.4658331
CGTTAAA550.050.4658331
GTTAAAG550.050.4658332
TCGAGGA150.00810403350.4658332
AGCTACC150.00810403350.4658331
ACGATTC251.9999225E-550.30216248
ACAGCAT204.0418608E-450.30216248
TATCTCA150.00820884450.3021648
ACTACTC150.00820884450.3021646
ATTATCT150.00820884450.3021646
CACAACC309.951455E-750.3021647
CCCGGTT150.00820884450.3021648