##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526629_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93697 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.595003041719586 39.0 38.0 40.0 36.0 40.0 2 38.21329391549356 39.0 38.0 40.0 35.0 40.0 3 38.34481360128926 39.0 38.0 40.0 35.0 40.0 4 37.85980340886048 39.0 38.0 40.0 33.0 40.0 5 38.18535278610841 39.0 38.0 40.0 35.0 40.0 6 38.176259645452895 39.0 38.0 40.0 35.0 40.0 7 38.39017257756385 39.0 38.0 40.0 36.0 40.0 8 38.57707290521575 39.0 38.0 40.0 36.0 40.0 9 38.53072136781327 39.0 38.0 40.0 36.0 40.0 10 37.56248332390578 39.0 38.0 40.0 33.0 40.0 11 37.60887755210946 39.0 37.0 40.0 33.0 40.0 12 37.588183186227944 39.0 37.0 40.0 33.0 40.0 13 37.68885876815693 39.0 38.0 40.0 34.0 40.0 14 37.72816632336147 39.0 38.0 40.0 34.0 40.0 15 37.76579826461893 39.0 38.0 40.0 34.0 40.0 16 37.60178020641003 39.0 38.0 40.0 33.0 40.0 17 37.55162918770078 39.0 38.0 40.0 33.0 40.0 18 37.4832278514787 39.0 37.0 40.0 33.0 40.0 19 37.336051314343045 39.0 37.0 40.0 33.0 40.0 20 37.35400279624748 39.0 37.0 40.0 33.0 40.0 21 37.13780590627235 39.0 36.0 40.0 33.0 40.0 22 37.05911608696116 39.0 36.0 40.0 33.0 40.0 23 36.877445382456216 39.0 36.0 40.0 32.0 40.0 24 36.69396031890029 39.0 36.0 40.0 31.0 40.0 25 36.614107175256414 39.0 36.0 40.0 31.0 40.0 26 36.13561800271086 38.0 35.0 40.0 30.0 40.0 27 36.00286028368038 38.0 35.0 40.0 30.0 40.0 28 35.74653404057761 38.0 35.0 40.0 29.0 40.0 29 35.667822875865824 38.0 35.0 40.0 29.0 40.0 30 35.49299337225311 38.0 35.0 40.0 29.0 40.0 31 34.856644289571705 38.0 34.0 40.0 27.0 40.0 32 34.63932676606508 38.0 33.0 39.0 27.0 40.0 33 34.524712637544425 38.0 33.0 39.0 26.0 40.0 34 34.594149225695595 38.0 33.0 40.0 26.0 40.0 35 34.386287714654685 38.0 33.0 39.0 26.0 40.0 36 33.90170443023789 38.0 33.0 39.0 23.0 40.0 37 33.421859824754264 37.0 33.0 39.0 20.0 40.0 38 33.442500827134275 37.0 33.0 39.0 22.0 40.0 39 33.392157699819634 37.0 33.0 39.0 21.0 40.0 40 32.934736437666096 37.0 32.0 39.0 17.0 40.0 41 32.27552643094229 36.0 31.0 39.0 11.0 40.0 42 32.14636541191287 36.0 31.0 39.0 10.0 40.0 43 32.0222845982262 36.0 31.0 39.0 8.0 40.0 44 31.553368837849664 36.0 30.0 39.0 4.0 40.0 45 31.452191639006585 36.0 30.0 39.0 4.0 40.0 46 30.77594800260414 35.0 30.0 39.0 4.0 40.0 47 30.47352636690609 35.0 29.0 39.0 4.0 40.0 48 30.143131583722 35.0 29.0 39.0 4.0 40.0 49 30.016638739767547 35.0 29.0 39.0 4.0 40.0 50 29.608237190091465 35.0 28.0 39.0 4.0 40.0 51 28.86582281182962 35.0 27.0 38.0 4.0 40.0 52 28.615857498105594 34.0 26.0 38.0 4.0 40.0 53 28.251897072478307 34.0 25.0 38.0 4.0 40.0 54 27.89908961866442 34.0 23.0 38.0 4.0 40.0 55 27.287661291183284 33.0 18.0 38.0 4.0 40.0 56 15.985933381004727 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.26029090721510073 1 2 0.16600543836554493 1 3 -0.4443997616148607 1 4 -0.41087501735189136 1 5 0.131415482950608 1 6 -0.1960016500737538 1 7 -0.6147385985911527 1 8 -0.767507063669342 1 9 -0.4952323242591987 1 10 -0.3049854313507012 1 11 -0.28263754620028436 1 12 -0.20160868107957697 1 13 -0.9840573823159815 1 14 -0.2504488602311312 1 15 -0.41461316240045676 1 16 -0.3259012100822716 1 17 -0.24752709656252847 1 18 -0.582472512530039 1 19 -0.454749712520055 1 20 0.11853420928667902 1 21 -0.0012289378041927534 1 22 0.2662386617366579 1 23 -0.6360510467642442 1 24 0.4404307824438263 1 25 -0.4389292518439376 1 26 -0.26596142741340145 1 27 -0.7175685739743827 1 28 -0.5459593696579432 1 29 -0.07832185622579146 1 30 -0.7349246613842979 1 31 1.0804762191246624 1 32 0.31197564775698794 1 33 -0.21945282839293867 1 34 0.1513096423163347 1 35 0.13951181085799647 1 36 0.5688137702158116 1 37 0.20299634947244982 1 38 0.5501556736777999 1 39 0.05110896156394773 1 40 0.13658486552360927 1 41 1.1531692282573616 1 42 1.2613380105019338 1 43 1.5737887677631939 1 44 0.8848300513156744 1 45 1.090059140636658 1 46 2.09405896442496 1 47 1.9486816998716563 1 48 2.2352791450713916 1 49 2.6531372620643445 1 50 2.311061183292175 1 51 2.2221810180972135 1 52 2.6593043802306617 1 53 1.9713096983243261 1 54 2.359814402343531 1 55 2.491611976351681 1 56 2.1917372561895334 2 1 0.18450847944475157 2 2 0.40699700493851054 2 3 0.24829126828547032 2 4 -0.04235981162738511 2 5 0.7802782480924435 2 6 -0.11537297642182409 2 7 0.3461609669595802 2 8 0.07059669303907157 2 9 0.19886427681414176 2 10 0.17393100105410753 2 11 -0.5811297516692306 2 12 -0.5085343135866083 2 13 -0.22223780654291403 2 14 -0.5094572936581656 2 15 -1.0279533106243264 2 16 -0.8513292703940536 2 17 -0.33275582132613835 2 18 -0.5018438388781092 2 19 -0.6378573026043881 2 20 -0.5599733296859739 2 21 -0.009662364842277782 2 22 -0.007081041815609979 2 23 -0.0063551612544046066 2 24 -0.37991166580560076 2 25 0.2314004184857339 2 26 -1.074164851895901 2 27 -0.97478809863577 2 28 -0.41728571772847545 2 29 -0.6520504532102009 2 30 -0.9116432915913038 2 31 0.28070622168024784 2 32 -0.06701745216633981 2 33 0.20911860017849193 2 34 -0.19739978778844147 2 35 0.3439585269326173 2 36 -0.7005447782125032 2 37 -1.1367685368040625 2 38 -0.23734752080213894 2 39 -0.46269119177109275 2 40 -0.7697529826747065 2 41 -0.02444385633246071 2 42 0.32867764760900897 2 43 0.2462906844511572 2 44 -0.059458218553992026 2 45 0.18078237089477156 2 46 -0.8802574168681616 2 47 -1.2741652206496816 2 48 -0.7626763877678613 2 49 -0.9755363898651197 2 50 -1.6108657270068285 2 51 -1.685434622076567 2 52 -1.0729982009091295 2 53 -1.1185190775509604 2 54 -1.1875916799462694 2 55 -0.9184314685754842 2 56 -0.9400030964810533 3 1 -0.4913788516149893 3 2 0.22561516838425888 3 3 -0.47420462662421414 3 4 -0.032633905349754855 3 5 0.6165798774975215 3 6 0.28983101498906905 3 7 0.4828288967309575 3 8 0.41011923945816875 3 9 0.39249822906878507 3 10 -0.03928107422693472 3 11 -0.2853106282639004 3 12 -0.031333353627026383 3 13 0.009794528937696612 3 14 -0.17359775090207563 3 15 -0.1291279980059059 3 16 -0.4082321376417468 3 17 0.1415399977972669 3 18 -0.6828467440189456 3 19 -0.08385957619286444 3 20 -0.2762800115098969 3 21 0.022294184355651225 3 22 -0.10358224176508202 3 23 -0.18937903393344868 3 24 -0.25991671822444573 3 25 -0.03663040126922823 3 26 0.09370176424658183 3 27 0.17804757144127592 3 28 0.2694643138491415 3 29 0.03154181658896249 3 30 -0.0063761449448449525 3 31 0.4051220357512335 3 32 0.016600079727048467 3 33 0.7805471716070613 3 34 0.3289359454438454 3 35 0.5615914687034902 3 36 1.507867499165286 3 37 1.118125993952539 3 38 0.8175975341429194 3 39 0.4516703087973042 3 40 0.21824752256717517 3 41 -0.24030845150740276 3 42 0.7650803253910006 3 43 -0.05104416460783412 3 44 0.8137260684233993 3 45 0.2469690577711141 3 46 0.1546042731659405 3 47 0.35953441304995337 3 48 0.4747872979716874 3 49 1.1997825440745018 3 50 0.4400373928618073 3 51 -0.2826305296173004 3 52 0.014156024176131154 3 53 -0.16702229846797323 3 54 0.013115658692100851 3 55 0.6219247269531252 3 56 -0.6726038290817851 4 1 0.33620735528155876 4 2 1.248603674131381 4 3 0.8821172124562437 4 4 0.8984005774088644 4 5 0.9039361993366057 4 6 0.7955781414258496 4 7 1.0345530346619896 4 8 0.7779353314121877 4 9 0.8292798382641848 4 10 1.3285350177270843 4 11 0.2549192567935705 4 12 0.3249884854534386 4 13 0.6074956783629801 4 14 0.5045631686381569 4 15 0.7099524617642032 4 16 0.29291728764560787 4 17 0.026597469061634627 4 18 0.2826704973603711 4 19 0.8011978950715033 4 20 -0.20731449426851611 4 21 1.0107999314820901 4 22 0.5056131605337697 4 23 0.9485520005493129 4 24 1.1308878794767026 4 25 0.6300362653974432 4 26 1.1856557872350848 4 27 0.6952889507516247 4 28 1.4303838540790252 4 29 0.8591280234855176 4 30 0.993623855055155 4 31 1.899374909314453 4 32 1.510852953290268 4 33 1.5046851026415453 4 34 1.7197405431449937 4 35 1.6765339974391225 4 36 2.4503962347974735 4 37 3.1066317410789708 4 38 2.783114775522229 4 39 2.9114404237398332 4 40 3.919396947854537 4 41 3.104519134699494 4 42 2.351287221942723 4 43 3.0294156055071078 4 44 2.894185838538341 4 45 1.8331759543228365 4 46 2.522420365119963 4 47 2.1296493555786853 4 48 2.831109137052149 4 49 3.3262193256836987 4 50 2.738887967574449 4 51 3.4530016542907447 4 52 3.8302479781991217 4 53 3.5111386210722557 4 54 3.2430007161633654 4 55 3.4610051867232414 4 56 1.7526835272400518 5 1 -0.14903297909670243 5 2 0.19296104090254573 5 3 -0.18515029015608775 5 4 -0.2879891822567515 5 5 0.12480871762291201 5 6 -0.7012870623359078 5 7 0.20317894897755906 5 8 0.0041631266055048854 5 9 -0.2854014574515915 5 10 -0.30234272521962424 5 11 -0.7311047766442584 5 12 -0.6490188290711174 5 13 -1.0189411032462132 5 14 -0.5303114395123174 5 15 -0.3046765873476005 5 16 -0.44323736230214195 5 17 -0.9668717054420242 5 18 -0.22412156115582604 5 19 -0.8804896702367557 5 20 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7 29 2.226805630465158 7 30 1.6487962688482583 7 31 1.854782747564002 7 32 2.168518096622222 7 33 2.3588604246191096 7 34 2.7734727132045407 7 35 2.655622766307701 7 36 3.567693008097571 7 37 3.7831308409266384 7 38 3.6730192208899055 7 39 3.731271472071093 7 40 4.144850486145629 7 41 3.691695426071881 7 42 4.074871213079685 7 43 4.189227265941955 7 44 4.493964262579333 7 45 4.13881229278012 7 46 3.3976454032033345 7 47 3.753108710238706 7 48 2.7953800136620366 7 49 2.9823611342974665 7 50 2.2669311748864587 7 51 2.4963474512717703 7 52 2.736216680594911 7 53 2.9364259773940944 7 54 2.197023704669114 7 55 2.3227971822917226 7 56 1.7049062110788569 8 1 0.2834095783458537 8 2 1.0223816203231237 8 3 0.4900495100437112 8 4 0.8037940345264616 8 5 0.6813771491913485 8 6 -0.1593290203778679 8 7 0.708798329596938 8 8 0.44422306666544387 8 9 0.5505126284624922 8 10 0.6354694625925674 8 11 -0.04266821320769054 8 12 -0.17886398391627267 8 13 0.06347647917137067 8 14 0.1938394096385352 8 15 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-0.26422662325259694 120 32 -0.7190321041809966 120 33 -0.041675050615161524 120 34 -0.2840908744795243 120 35 -0.1617801788060902 120 36 -0.2890673667350967 120 37 -0.3362801363156649 120 38 -0.30462468807930065 120 39 -0.28894271802261784 120 40 -0.3920973050190284 120 41 0.07616664427803954 120 42 0.32025273918410235 120 43 -0.02000968184921348 120 44 0.5876724285766564 120 45 -0.0032225131101100146 120 46 -0.682943619554365 120 47 -0.4849100313944916 120 48 -0.11640044149191553 120 49 -1.4776120919408235 120 50 -0.43467525081635117 120 51 -1.4630902997322437 120 52 -2.9130470409579665 120 53 -2.3969073559392378 120 54 -2.0585318508864425 120 55 -2.3711787213227353 120 56 -2.5503816068595633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 124.0 5 7.0 6 17.0 7 22.0 8 55.0 9 75.0 10 63.0 11 116.0 12 130.0 13 182.0 14 230.0 15 268.0 16 350.0 17 429.0 18 542.0 19 567.0 20 636.0 21 766.0 22 762.0 23 895.0 24 1186.0 25 1442.0 26 1293.0 27 1465.0 28 1768.0 29 2038.0 30 2360.0 31 2850.0 32 3412.0 33 4257.0 34 5604.0 35 7694.0 36 10611.0 37 15219.0 38 16692.0 39 9570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.90126317363031 0.8905213928010849 0.1398780604999306 0.06833737306866838 8 99.44075050428509 0.4695988131957266 0.03735445104966007 0.052296231469524106 9 97.90708347118905 1.2775222258983745 0.3137773888171446 0.5016169140954353 10 49.0762777890434 33.86234351153185 6.814519141487987 10.246859557936753 11 33.995752265280636 29.692519504359797 18.129715999445022 18.182012230914545 12 36.147368645741054 16.94718080621578 24.241971461199398 22.663479086843765 13 24.606977811456076 17.428519589741402 29.381943925632626 28.5825586731699 14 24.06800644631098 22.630393715914064 29.155682679274687 24.14591715850027 15 27.316776417601417 21.504423834274313 28.030780067664917 23.148019680459353 16 27.118264192023222 22.13518042199857 25.35513410247927 25.39142128349894 17 28.099085349584296 22.264320095627394 23.17363416117912 26.462960393609187 18 27.421368880540463 23.346531906037544 23.609080333415157 25.623018880006832 19 27.837604192236675 22.2088220540679 23.06157080803014 26.892002945665283 20 26.902675645965186 24.5824306007663 22.629326445884075 25.885567307384445 21 26.202546506291558 21.64637074826302 26.813024963446004 25.338057781999424 22 28.005165586945154 20.699702231661632 24.048795585771156 27.246336595622054 23 27.363736298920987 24.482107217947213 24.298536772788886 23.855619710342914 24 25.958141669423778 25.943199889003914 24.417003746117803 23.681654695454498 25 27.541508728845233 23.018525439111635 24.248571886177995 25.191393945865144 26 25.62467965146079 21.65983256449684 26.556893900563814 26.15859388347856 27 23.871939494936548 19.601119514592146 27.40674272529163 29.120198265179674 28 24.416169903638657 23.38738969724163 27.95760955494306 24.238830844176658 29 26.548757681004542 20.70531659096981 28.566390595778785 24.17953513224686 30 23.93789364500572 21.03360886255975 31.110921009014405 23.917576483420127 31 24.067281884577135 20.834714543879723 31.27987424746303 23.818129324080115 32 25.49097252694329 19.76625961878057 33.28552928711324 21.4572385671629 33 26.288858439667724 19.44099511860923 32.20005138306072 22.07009505866233 34 23.97272007023404 22.12586454251515 35.78510096143552 18.116314425815293 35 22.623445882995473 23.82925862862895 35.19880917959756 18.348486308778018 36 23.713979646491698 22.909480449919656 32.70487412961971 20.671665773968932 37 23.279290964115866 22.1714223951578 34.18396022481625 20.365326415910072 38 24.446016788344643 22.230083941723215 35.12473332690102 18.19916594303112 39 24.08709822672985 20.08062268156185 34.53802774621009 21.29425134549821 40 23.982124057323333 21.212642072921813 34.866682422705885 19.938551447048965 41 22.253814295830562 21.438381150619083 36.038926203300356 20.268878350249995 42 21.130508492762644 21.11441355408436 35.69213601296179 22.06294194019121 43 21.44544419868147 19.292632121459402 37.50402645650353 21.757897223355595 44 21.676173289076512 19.73510360607135 35.83620680394874 22.752516300903398 45 21.890745017994305 20.424343342106678 35.804909491325134 21.880002148573887 46 21.066319895968793 20.042128340981634 35.43509333791873 23.456458425130844 47 21.081754789354363 21.782295236969627 36.5452181667759 20.59073180690011 48 22.18850871530019 23.13965999569615 34.77404777275662 19.897783516247042 49 21.55835493830481 22.225329991286294 34.88817410200416 21.328140968404746 50 20.89150872361939 22.591054794225844 35.393584751414494 21.12385173074027 51 21.517449050922192 23.372586976394043 35.40571059848362 19.70425337420014 52 22.329950822671073 22.61834303608131 34.09484660332082 20.956859537926803 53 21.185081534901244 21.97190678650234 35.41144179538238 21.431569883214035 54 22.593772877998365 22.82416777916541 33.93695361956849 20.645105723267733 55 21.909519605520984 23.299455222755753 33.43274261966796 21.358282552055297 56 22.199972007794752 23.74169654296265 31.83144385947913 22.226887589763468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 10.0 9 19.0 10 73.5 11 128.0 12 96.0 13 88.0 14 112.0 15 157.0 16 202.0 17 391.0 18 580.0 19 734.0 20 888.0 21 870.5 22 1034.5 23 1216.0 24 1528.5 25 1841.0 26 2093.0 27 2345.0 28 2698.5 29 3196.5 30 3341.0 31 3480.0 32 3619.0 33 3007.0 34 2395.0 35 2366.5 36 2338.0 37 1938.5 38 1444.5 39 1350.0 40 1417.0 41 1484.0 42 1572.5 43 1661.0 44 1865.5 45 2070.0 46 2402.5 47 2603.5 48 2472.0 49 2761.0 50 3050.0 51 3311.0 52 3572.0 53 3938.5 54 4389.0 55 4473.0 56 4710.0 57 4947.0 58 5454.0 59 5961.0 60 6212.5 61 6464.0 62 6298.0 63 5656.0 64 5180.0 65 4474.5 66 3769.0 67 3358.0 68 2947.0 69 2478.0 70 2009.0 71 1830.0 72 1343.0 73 1035.0 74 950.5 75 866.0 76 658.0 77 450.0 78 349.5 79 215.5 80 182.0 81 130.5 82 79.0 83 56.5 84 34.0 85 26.5 86 19.0 87 14.5 88 7.5 89 5.0 90 4.0 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.04695988131957266 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04482534125959209 26 0.052296231469524106 27 0.09071795254917446 28 0.0971215727291162 29 0.1302069436588151 30 0.1921086053982518 31 0.1921086053982518 32 0.27749020779747485 33 0.29990287842727087 34 0.3148446588471349 35 0.33725732947693093 36 0.3703427004066299 37 1.2561768252985688 38 0.44611887253594035 39 0.4514552226858918 40 0.6521019883240659 41 0.5282986648451925 42 0.533635014995144 43 0.600873026884532 44 0.6446310981141339 45 0.6531692583540561 46 0.6915909794337065 47 0.6681110387739202 48 0.8079234127026479 49 0.789779822192813 50 0.7801743919229004 51 0.759896261353085 52 0.819663383032541 53 0.8452778637523081 54 0.867690534382104 55 0.8698250744420846 56 0.8687578044120943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 93697.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.78679146610884 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24324890098387 28.242099533603 2 11.851057148838184 9.667331931652027 3 5.638999371990789 6.89990074388721 4 3.312748586979276 5.404655431870817 5 2.3655013606866233 4.824060535556101 6 1.4679715302491103 3.5924309209473084 7 1.248168306468495 3.563614630137571 8 0.8922964203474983 2.911512641813505 9 0.7352941176470588 2.699125905845438 >10 2.990893866443374 20.667684130761923 >50 0.16746912288046892 4.792042434656393 >100 0.08111785639522713 5.175192375422906 >500 0.005233410090014654 1.5603487838458008 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 921 0.9829556976210552 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 541 0.5773930862247457 No Hit CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 408 0.43544617223603743 No Hit CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 342 0.36500635025667844 No Hit CACAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 294 0.3137773888171446 No Hit CACAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 232 0.24760664695774678 No Hit CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 200 0.21345400599805756 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 185 0.19744495554820324 No Hit CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 178 0.18997406533827124 No Hit CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 161 0.17183047482843633 No Hit CACAAGGGGGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTG 156 0.1664941246784849 No Hit CACAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 154 0.16435958461850433 No Hit CACAAGGGGGAGGATCGTCCAGAGTTATACTACGCAGGCGCCCGCTCTCTGCGCTC 145 0.15475415434859174 No Hit CACAAGGGGAGGCTGTTCCCGTTATGTAAGTCAGTTCTTCAGGCTCATTTGCTGTG 142 0.15155234425862088 No Hit CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 141 0.15048507422863058 No Hit CACAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 139 0.14835053416865002 No Hit CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT 139 0.14835053416865002 No Hit CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 133 0.1419469139887083 No Hit CACAAGGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCC 129 0.13767783386874713 No Hit CACAAGGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAGTCTGTG 128 0.13661056383875683 No Hit CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 122 0.1302069436588151 No Hit CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 121 0.12913967362882484 No Hit CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 120 0.12807240359883454 No Hit CACAAGGGGAAAGTATATGACCATGAGATTGCTTCAGTATTTAGTTCTTTCTAAAA 119 0.12700513356884424 No Hit CACAAGGGGGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGACACTG 111 0.11846697332892195 No Hit CACAAGGGGCAACCATCTGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAA 109 0.11633243326894137 No Hit CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 108 0.1152651632389511 No Hit CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 108 0.1152651632389511 No Hit CACAAGGGGAGACTTGTATATCCATATCTCCATGGCAGTGAGAAACGGTAGCTTAG 108 0.1152651632389511 No Hit CACAAGGGGAGGAGGGGCCCATCCCTCTCCCAGGCATTCATCAGCCTGCTGCCTTC 107 0.1141978932089608 No Hit CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 105 0.11206335314898022 No Hit CACAAGGGGCTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCT 104 0.11099608311898994 No Hit CACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.10779427302901906 No Hit CACAAGGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 98 0.10459246293904821 No Hit CACAAGGGGAACTTTGCTTGCGGTAGATTTTCTTTAAAAGAATGGGCAGTTACATG 95 0.10139065284907735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0010672700299902878 0.0 0.0 0.0 0.0 6 0.0010672700299902878 0.0 0.0 0.0 0.0 7 0.0010672700299902878 0.0 0.0 0.0 0.0 8 0.0021345400599805755 0.0 0.0 0.0 0.0 9 0.004269080119961151 0.0 0.0 0.0 0.0 10 0.011739970329893165 0.0 0.0 0.0 0.0 11 0.016009050449854317 0.0 0.0 0.0 0.0 12 0.017076320479844604 0.0 0.0 0.0 0.0 13 0.01921086053982518 0.0 0.0 0.0 0.0 14 0.022412670629796044 0.0 0.0 0.0 0.0 15 0.022412670629796044 0.0 0.0 0.0 0.0 16 0.02347994065978633 0.0 0.0 0.0 0.0 17 0.025614480719766908 0.0 0.0 0.0 0.0 18 0.02881629080973777 0.0 0.0 0.0 0.0 19 0.030950830869718348 0.0 0.0 0.0 0.0 20 0.0352199109896795 0.0 0.0 0.0 0.0 21 0.03735445104966007 0.0 0.0 0.0 0.0 22 0.04482534125959209 0.0 0.0 0.0 0.0 23 0.04909442137955324 0.0 0.0 0.0 0.0 24 0.050161691409543525 0.0 0.0 0.0 0.0 25 0.050161691409543525 0.0 0.0 0.0 0.0 26 0.052296231469524106 0.0 0.0 0.0 0.0 27 0.05549804155949497 0.0 0.0 0.0 0.0 28 0.05763258161947554 0.0 0.0 0.0 0.0 29 0.060834391709446406 0.0 0.0 0.0 0.0 30 0.070439821979359 0.0 0.0 0.0 0.0 31 0.077910712189291 0.0 0.0 0.0 0.0 32 0.08538160239922303 0.0 0.0 0.0 0.0 33 0.08644887242921331 0.0 0.0 0.0 0.0 34 0.09071795254917446 0.0 0.0 0.0 0.0 35 0.11099608311898994 0.0 0.0 0.0 0.0 36 0.11206335314898022 0.0 0.0 0.0 0.0 37 0.1141978932089608 0.0 0.0 0.0 0.0 38 0.12380332347887339 0.0 0.0 0.0 0.0 39 0.12700513356884424 0.0 0.0 0.0 0.0 40 0.13340875374878597 0.0 0.0 0.0 0.0 41 0.1419469139887083 0.0 0.0 0.0 0.0 42 0.14514872407867915 0.0 0.0 0.0 0.0 43 0.14941780419864029 0.0 0.0 0.0 0.0 44 0.15261961428861115 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCTA 45 1.1823431E-10 50.603046 36 CGAACTG 15 0.008017454 50.603043 34 GAGCTCA 20 3.924534E-4 50.603043 37 CCGTCGC 20 3.924534E-4 50.603043 36 GCCGTCG 20 3.924534E-4 50.603043 35 CTGCCGT 20 3.966881E-4 50.493217 33 GCGAACT 15 0.008086662 50.493214 33 AAAGGAT 15 0.008086662 50.493214 33 GGGTAGT 15 0.008104033 50.46583 31 CGTTAAA 55 0.0 50.46583 31 GTTAAAG 55 0.0 50.46583 32 TCGAGGA 15 0.008104033 50.46583 32 AGCTACC 15 0.008104033 50.46583 31 ACGATTC 25 1.9999225E-5 50.302162 48 ACAGCAT 20 4.0418608E-4 50.302162 48 TATCTCA 15 0.008208844 50.30216 48 ACTACTC 15 0.008208844 50.30216 46 ATTATCT 15 0.008208844 50.30216 46 CACAACC 30 9.951455E-7 50.30216 47 CCCGGTT 15 0.008208844 50.30216 48 >>END_MODULE