FastQCFastQC Report
Fri 17 Jun 2016
SRR1526628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230256
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.48424362448752695No Hit
AGGACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG6610.28707178097422No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT5890.2558022375095546No Hit
AGGACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5380.23365297755541659No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4980.2162810089639358No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.2075950246681954No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4380.19022305607671464No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4310.18718296157320546No Hit
AGGACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4180.18153707178097422No Hit
AGGACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.16633659926342853No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3320.1441873393092905No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3250.1411472448057814No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3200.1389757487318463No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC2800.12160378014036552No Hit
AGGACTGGGAGCACAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACAATTCCTTATTCCG2650.11508929191856021No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2540.110312000555903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC258.480927E-658.19069356
CCTATTA150.00462199358.19069358
CGCTAGT201.9746575E-458.1906954
GAGTTAT150.004625992458.17798652
ACAATTC258.502922E-658.16528349
CTAAGTA258.524963E-658.13989639
GCGATCT201.983204E-458.13989339
CGATCTG201.983204E-458.13989340
TTATATA150.004650043358.10185638
TAGACAA150.004674187458.0259232
AACTGAC150.004706524357.92498428
CTGATAT150.004706524357.92498428
TCCACCG202.0198555E-457.9249828
TCACGGC150.004714634757.89980327
CTCCCAT150.004714634757.89980323
CGTCGAC150.004714634757.89980325
CTAATGG150.004714634757.89980322
AACGGGT351.6645572E-857.8620820
ATTAATG150.004730886357.8495111
GTATCAT150.004730886357.8495118
Produced by FastQC (version 0.11.5)