FastQCFastQC Report
Fri 17 Jun 2016
SRR1526627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223316
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.395403822386215No Hit
AGGACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG5190.23240609718963262No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4830.2162854430493113No Hit
AGGACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.2050905443407548No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4300.19255225778717153No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3850.1724014401117699No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.16031094950652885No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3430.153594010281395No Hit
AGGACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3210.14374249941786527No Hit
AGGACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.13478658045102007No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2750.12314388579412133No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2640.1182181303623565No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC2500.11194898708556485No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2330.10433645596374644No Hit
AGGACTGGGAGCACAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACAATTCCTTATTCCG2270.10164968027369288No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2240.1003062924286661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGA258.4162E-658.26490836
CACTAAG258.461384E-658.21232235
TACCGTA150.00461500658.2123239
AAGTTAG258.563784E-658.09435330
TATTATG258.563784E-658.09435356
CGCTATA201.9907669E-458.0943556
CACAGTT150.004652230558.09434530
TAAGGTG150.004652230558.09434528
CAAACTA150.004660533358.068250
ACGTATG150.004660533358.068229
ACAATTC351.6210834E-858.0551349
AAGATTA201.9974257E-458.0551348
GTCATAG150.004664688458.05512647
GGTAATC150.004664688458.05512648
CATTCTA150.004664688458.05512648
ATTCTAG150.004664688458.05512649
CCCTCAA150.004668846758.0420745
CGTAAGG150.004673007458.0290142
ACCGTAA150.004673007458.0290140
TAGTATT150.004698030757.95080623
Produced by FastQC (version 0.11.5)