FastQCFastQC Report
Fri 17 Jun 2016
SRR1526626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206871
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11810.5708871712323139No Hit
AGGACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.29293617761793583No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG5500.26586616780505723No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5500.26586616780505723No Hit
AGGACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA4910.2373459788950602No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4120.19915792933760654No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.1880398895930314No Hit
AGGACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG3330.16096987978015284No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3190.1542023773269332No Hit
AGGACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.15178541216506905No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3110.15033523306795055No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2850.13776701422625695No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2750.13293308390252861No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2130.10296271589541309No Hit
AGGACTGGGAGAACTTGTATGTGGAGGTGGGGTTGGGGTATGTGGTATATGTGTGT2070.10006235770117611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTCA150.00799052850.66831237
ATTGGTC150.00800610150.64336835
TGACCAA203.9395172E-450.5935634
GTATTAG150.00811573850.46946332
TCGGCTA150.00811573850.46946333
CAATACA150.00811573850.46946332
AAATCAG301.0120766E-650.2230940
TAACGCG150.00827429550.2230940
CAAATCA301.0120766E-650.2230939
CGATGCA150.00827429550.2230947
GTACGTA451.2914825E-1050.2230939
TCAAATC301.0120766E-650.2230938
TAAAGTA150.00829027750.19858650
TTAAAGT150.00829827750.18634449
TAGCGAC150.00844324949.96697630
AAAAGAG150.00845135849.95484528
AACATAA150.00845135849.95484528
CTCAATT150.0084919949.8942826
TCGGTAG150.0084919949.8942826
GTCATAA150.00851643849.8580125
Produced by FastQC (version 0.11.5)