FastQCFastQC Report
Fri 17 Jun 2016
SRR1526625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84162
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37934.506784534588056No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18732.22546992704546No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.8115301442456215No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.47883843064565956No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.38616002471424155No Hit
CAAAGTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.3160571279199639No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.28754069532568144No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.27447066371996864No Hit
CAAAGTGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTAGTGGTCCA2280.27090610964568335No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.2637770014971127No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1940.23050783013711654Illumina Single End Adapter 2 (96% over 31bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.2269432760628312No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1850.21981416791426062No Hit
CAAAGTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.193674104702835No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.17585133433140848No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.1734749649485516No Hit
CAAAGTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.1699104108742663No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.1699104108742663No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA1350.1604049333428388No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1290.1532758251942682No Hit
CAAAGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1532758251942682No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1250.14852308642855444No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA1120.1330766854399848Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.12832394667427105No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.1235712079085573No Hit
CAAAGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.120006653834272No Hit
CAAAGTGGGGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCTTTTCAT1010.120006653834272No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.11644209975998669No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.11406573037712983No Hit
CAAAGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG860.10218388346284546No Hit
CAAAGTGGGTGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.10099569877141702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG150.004591959558.2538948
TCCTAAT150.004591959558.2538947
CCTAATA150.004591959558.2538948
TTCTAGT150.004591959558.2538947
TAATATC150.004591959558.2538950
CCTAGTG150.004591959558.2538953
CCCTAGT150.004591959558.2538952
GCTGAGT150.004613789358.18420844
ATTTCTA150.004613789358.18420845
TGCTATT201.974295E-458.14943341
ACCGTAG150.00462473358.1494339
CGTAGCG150.00462473358.1494341
CGAGGTC150.00462473358.1494340
GCTATTT150.00462473358.1494342
TGTGCTA150.00462473358.1494339
CTATTTC150.00462473358.1494343
TATGTGC150.00464667858.07999837
TACCGTA150.00464667858.07999838
CCCCGAG150.00464667858.07999837
CTATGTG150.00464667858.07999836
Produced by FastQC (version 0.11.5)