FastQCFastQC Report
Fri 17 Jun 2016
SRR1526624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82231
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30633.724872614950566No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15551.891014337658547No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.605611022607046No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.46454500127689075No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.33077549829139863No Hit
CAAAGTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.26024248762632096No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.2565942284539894No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.21767946394911894No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1740.21159903199523292Illumina Single End Adapter 2 (96% over 31bp)
CAAAGTGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTAGTGGTCCA1650.20065425447823812No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.19822208169668373No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1630.19822208169668373No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.16538774914569931No Hit
CAAAGTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.1617394899733677No Hit
CAAAGTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.15930731719181332No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.1580912308010361No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA1050.1276890710316061No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.12647298464082887No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.12647298464082887No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG980.11917646629616568No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.11552820712383409No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG940.11431212073305687No Hit
CAAAGTGGGGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCTTTTCAT900.10944777516994808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAC258.203477E-658.4608334
TTCGCTA303.52009E-758.46082333
ACCCGCT150.004527465458.46082333
TTCTATG150.004527465458.46082334
TAGAGCG150.004527465458.46082334
CGCTGAA150.004549641658.38878636
CTATGTG150.004549641658.38878636
TCTATGT150.004560759858.35283335
TTACCCG150.004583057558.28105531
TATGTGC150.004583057558.28105537
ACCGTAG201.9520246E-458.28105539
ATGTGCT150.004583057558.28105538
CTGAATT150.004583057558.28105538
AACCAAT150.004583057558.28105531
TACCGTA201.9520246E-458.28105538
GCTGAAT150.004583057558.28105537
GTAATTC150.004583057558.28105530
GTTACCC150.004583057558.28105530
TGTGCTA150.004583057558.28105539
CTACCGT201.9520246E-458.28105537
Produced by FastQC (version 0.11.5)