##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526624_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82231 Sequences flagged as poor quality 0 Sequence length 64 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.132018338582775 38.0 37.0 39.0 33.0 40.0 2 36.27580839342827 38.0 36.0 39.0 30.0 40.0 3 36.49281900986246 38.0 36.0 39.0 30.0 40.0 4 36.13192105167151 38.0 36.0 39.0 29.0 40.0 5 36.20048400238353 38.0 36.0 39.0 30.0 40.0 6 34.75221023701524 37.0 33.0 39.0 27.0 40.0 7 37.159574856197786 38.0 38.0 40.0 33.0 40.0 8 36.94837713271151 38.0 38.0 40.0 32.0 40.0 9 37.03246950663375 38.0 38.0 40.0 32.0 40.0 10 35.75736644331213 38.0 35.0 40.0 28.0 40.0 11 35.593267745740654 38.0 35.0 39.0 28.0 40.0 12 35.74327200204303 38.0 35.0 40.0 29.0 40.0 13 35.54396760345855 38.0 35.0 39.0 28.0 40.0 14 35.97069231798227 38.0 35.0 40.0 30.0 40.0 15 35.74952268609162 38.0 35.0 39.0 29.0 40.0 16 36.20483759166251 39.0 36.0 40.0 30.0 40.0 17 36.15961133878951 39.0 36.0 40.0 30.0 40.0 18 36.370091571305224 38.0 36.0 40.0 31.0 40.0 19 36.042283323807325 38.0 35.0 40.0 30.0 40.0 20 36.247546545706605 38.0 36.0 40.0 30.0 40.0 21 36.300604394936215 39.0 36.0 40.0 31.0 40.0 22 36.1963736303827 38.0 36.0 40.0 30.0 40.0 23 35.923240627014145 38.0 35.0 40.0 30.0 40.0 24 35.80819885444662 38.0 35.0 40.0 29.0 40.0 25 35.75320742785568 38.0 35.0 40.0 29.0 40.0 26 35.77660493001423 39.0 35.0 40.0 29.0 40.0 27 35.28034439566587 39.0 35.0 40.0 27.0 40.0 28 35.26382994247911 39.0 35.0 40.0 27.0 40.0 29 35.196762778027754 39.0 35.0 40.0 27.0 40.0 30 34.9701085965147 38.0 35.0 40.0 26.0 40.0 31 34.63334995318068 38.0 35.0 40.0 24.0 40.0 32 34.49759822937821 38.0 34.0 40.0 24.0 40.0 33 34.68783062348749 38.0 35.0 40.0 25.0 40.0 34 34.1506731038173 38.0 34.0 40.0 22.0 40.0 35 34.0998285318189 38.0 33.0 40.0 22.0 40.0 36 33.75746373022339 39.0 34.0 40.0 17.0 40.0 37 33.655397599445465 38.0 33.0 40.0 17.0 40.0 38 33.68706448906131 38.0 33.0 40.0 18.0 40.0 39 33.205518600041344 38.0 33.0 40.0 10.0 40.0 40 33.401308508956475 38.0 33.0 40.0 16.0 40.0 41 33.401734139193245 38.0 33.0 40.0 11.0 40.0 42 33.11197723486276 38.0 33.0 40.0 6.0 40.0 43 33.137794748938965 38.0 33.0 40.0 7.0 40.0 44 32.93652029040143 38.0 33.0 39.0 4.0 40.0 45 32.787318651116976 38.0 32.0 39.0 4.0 40.0 46 32.61902445549732 38.0 33.0 39.0 4.0 40.0 47 32.46829054736048 38.0 33.0 39.0 4.0 40.0 48 32.28049032603276 38.0 32.0 39.0 4.0 40.0 49 32.004329267551164 37.0 32.0 39.0 4.0 40.0 50 31.5811312035607 37.0 32.0 39.0 4.0 40.0 51 31.032882976006615 37.0 31.0 39.0 4.0 40.0 52 30.872906811299874 37.0 31.0 39.0 4.0 40.0 53 30.508676776398197 36.0 30.0 39.0 4.0 40.0 54 30.099256971215237 36.0 29.0 39.0 4.0 40.0 55 29.530736583526895 36.0 27.0 39.0 4.0 40.0 56 28.381328209556006 35.0 15.0 39.0 4.0 40.0 57 27.86557381036349 35.0 8.0 39.0 4.0 40.0 58 27.4988264766329 35.0 4.0 39.0 4.0 40.0 59 27.006311488368134 35.0 4.0 39.0 4.0 40.0 60 26.489341002784837 34.0 4.0 39.0 4.0 40.0 61 25.79712030742664 34.0 4.0 39.0 4.0 40.0 62 25.57399277644684 34.0 4.0 39.0 4.0 40.0 63 25.214967591297686 34.0 4.0 39.0 4.0 40.0 64 25.179020077586312 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.08071178231134724 2 2 0.2171760845056454 2 3 -0.42722737480356443 2 4 0.06283666841248703 2 5 0.10913745902592353 2 6 0.2296760213671476 2 7 0.02103029819432578 2 8 0.3003611927142842 2 9 0.29475616026334706 2 10 -0.026349265969294322 2 11 0.19263715974103945 2 12 0.5428371772015694 2 13 0.4034422371566251 2 14 0.5597149999673903 2 15 0.43214524086808837 2 16 -0.13798819044880162 2 17 0.8244892629227394 2 18 0.41341231470077844 2 19 0.30391490561723344 2 20 0.47563491570764427 2 21 0.049863323268859006 2 22 0.12697433944723002 2 23 -0.09255529843208166 2 24 -0.43176510718436845 2 25 0.5623662367270725 2 26 -0.8517513217602186 2 27 -0.3903011165497148 2 28 -0.50059573194752 2 29 -0.12211907093099228 2 30 -0.25138262849404214 2 31 0.5664524217536524 2 32 0.557493762210953 2 33 -0.3520795291819354 2 34 1.3935831902860585 2 35 1.356719992692831 2 36 0.2836435747134587 2 37 0.877931770409532 2 38 0.15310062230782506 2 39 -3.28152364463104 2 40 0.5219320519977799 2 41 0.28844675379992424 2 42 0.9145987097109156 2 43 0.8925380890806878 2 44 1.3311640958392275 2 45 0.6713230867591022 2 46 1.6077922352111145 2 47 1.2900441986015778 2 48 1.25045220548823 2 49 1.6400536230510383 2 50 0.9633867160738312 2 51 1.925882753336296 2 52 1.832924264119594 2 53 2.1823637475014444 2 54 1.6299151790951498 2 55 1.5170568966076026 2 56 1.9368353352885954 2 57 1.9769731507954589 2 58 2.1479128894835 2 59 1.8875895889258452 2 60 2.0611394254216364 2 61 1.5517830197502782 2 62 2.2699189459904616 2 63 1.951467899778244 2 64 1.9179835742763984 3 1 -0.3160058999584052 3 2 -0.3553729351021957 3 3 -0.68016855127415 3 4 -0.36134633812346095 3 5 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0.6120290986699288 4 22 0.3697550881103311 4 23 0.5185409582524088 4 24 0.4453739302487847 4 25 0.15782078218161644 4 26 0.7423663252986046 4 27 0.9091641240919941 4 28 0.3511422359669183 4 29 0.0596991108871876 4 30 -0.2856072274245278 4 31 -0.8584138884067798 4 32 -1.1572121201419847 4 33 -1.474539422230066 4 34 -0.7925130663984348 4 35 -5.203975194472942 4 36 -0.796704018869427 4 37 -1.8446618124781686 4 38 -1.7264448322376253 4 39 -0.9402134842032339 4 40 -0.9967845255423242 4 41 -1.0432377381786822 4 42 -0.5212301672944335 4 43 -0.43821057402092123 4 44 -0.3481139790270831 4 45 -0.17546835709116948 4 46 -0.7435446631846077 4 47 -1.020116229205911 4 48 -0.6101092918379756 4 49 -0.940427660371423 4 50 -0.5927630165464919 4 51 -0.2583418455941846 4 52 -0.45531102999805384 4 53 0.3056257795870039 4 54 0.1148082272234916 4 55 0.1571638484792608 4 56 0.34084603047576323 4 57 -0.03238513797459319 4 58 0.5203727825316271 4 59 0.945209909781461 4 60 1.0821287302344693 4 61 1.7727723245631104 4 62 1.3129670743327075 4 63 1.086762017425304 4 64 1.5784113817630292 5 1 0.1321868711259313 5 2 0.38358288819097197 5 3 0.08489168968970517 5 4 0.174530502572658 5 5 0.22735149162691215 5 6 0.3982089767179673 5 7 0.12577870358057908 5 8 0.42977295742016963 5 9 0.4029064083143794 5 10 0.7145437177302085 5 11 -2.0318844561342218 5 12 -2.357658924853716 5 13 -1.8546725750333124 5 14 -1.202297756942599 5 15 -1.084793100733613 5 16 -17.535862038783314 5 17 -2.93794872432035 5 18 -2.7755316966386943 5 19 -3.2925415082736293 5 20 -3.150445878055642 5 21 -2.2832337709905346 5 22 -2.2896096435435567 5 23 -2.279514901550435 5 24 -1.919220812358006 5 25 -2.3484771367669097 5 26 0.0616434337323426 5 27 0.7152977495027315 5 28 0.28693084494830146 5 29 0.8537845435268423 5 30 0.6804387747660527 5 31 -0.4183810296992192 5 32 -0.10600730086487431 5 33 -0.23740908269008543 5 34 0.13957893798272636 5 35 -4.752989433246217 5 36 0.07939127138249091 5 37 0.11960434305304801 5 38 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25 -0.43995934466828146 119 26 -0.5367034421432564 119 27 -0.31916568563315906 119 28 -0.42713471963561744 119 29 -0.5058400011635484 119 30 -0.863831328904439 119 31 -1.5745872499289746 119 32 -1.8258167713047726 119 33 -1.1690425934774211 119 34 -0.7745426647071056 119 35 -1.3345248773482083 119 36 -0.48407188356287634 119 37 -0.9538055756917032 119 38 -0.9027133311805429 119 39 -0.31627056665566045 119 40 -0.279025540341479 119 41 -0.5223603597431676 119 42 -0.5635134653916865 119 43 -0.7241513774035866 119 44 -0.29975245682835094 119 45 -0.34618717315881753 119 46 -0.5352994200556438 119 47 -0.1607081953792715 119 48 -0.8492741967005522 119 49 -0.588947744938018 119 50 0.02563021812581212 119 51 0.43668986687938727 119 52 1.0679447839554363 119 53 0.08099575844535423 119 54 0.28367714899939145 119 55 0.8632949267033609 119 56 1.1986684406237558 119 57 1.0823083081142002 119 58 0.5927829305231711 119 59 0.7483283030161338 119 60 0.5062830642725231 119 61 0.24343828650814103 119 62 -0.009971614885049718 119 63 0.05187829649507236 119 64 0.18200546210129787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 387.0 5 40.0 6 74.0 7 138.0 8 290.0 9 159.0 10 189.0 11 243.0 12 281.0 13 367.0 14 338.0 15 441.0 16 496.0 17 554.0 18 609.0 19 653.0 20 673.0 21 932.0 22 928.0 23 853.0 24 913.0 25 1105.0 26 1172.0 27 1512.0 28 1955.0 29 2207.0 30 2803.0 31 3266.0 32 3758.0 33 4605.0 34 5154.0 35 5646.0 36 6754.0 37 9142.0 38 11630.0 39 11939.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.50626892534446 0.27969986987875617 0.11066386156072527 0.10336734321606206 8 98.77830642119224 0.7045424124797702 0.3188084837127803 0.19834268261520305 9 96.73967238632632 1.8302100181196872 0.6737118604905693 0.7564057350634189 10 39.927764468387835 41.271539930196646 7.722148581435224 11.078547019980299 11 20.489839598205055 39.34404300081478 27.50787415938028 12.658243241599884 12 56.083472169862944 17.29882890880568 16.625117048315115 9.99258187301626 13 24.88599190086464 14.963943038513456 33.394948377132714 26.755116683489195 14 35.921975897167734 30.809548710340383 18.05158638469677 15.216889007795114 15 44.94413475816071 20.314744042257978 17.432633090723204 17.308488108858107 16 20.15691521712687 23.84989660625228 27.91509548716701 28.078092689453836 17 30.831801078869503 21.93538959852904 16.461892527063064 30.7709167955384 18 28.23022947550194 35.11327844730089 19.58871958263915 17.067772494558014 19 19.143631963614695 24.335104765842566 36.9532171565468 19.568046113995937 20 16.22591475282211 33.07099065784352 17.522625535227714 33.18046905410665 21 17.782831292335008 34.184188444747114 26.319757755590956 21.713222507326922 22 19.61060913767314 36.78539723461955 14.99312911189211 28.610864515815205 23 29.625080565723387 40.45919422115747 15.302015055149518 14.613710157969622 24 18.878984299942843 50.780138393997255 16.32514076542339 14.015736540636514 25 20.23560336854403 27.26841259796524 27.9681643382174 24.52781969527333 26 28.548838340834447 23.516603819486683 20.6665855735312 27.267972266147673 27 19.117073646244577 23.11497782326851 22.235219574011793 35.53272895647512 28 21.52817527493271 32.02859543788287 21.702329773112005 24.740899514072414 29 28.2077194691343 26.48727626932911 22.722513089005236 22.582491172531352 30 18.201642180152522 43.46173525327096 24.534025290548936 13.802597276027582 31 23.223644119439367 39.82327848872639 26.20840950639854 10.74466788543571 32 13.595673937666703 39.575188473577164 36.18570889205549 10.643428696700646 33 16.870421608181076 40.030228051143915 28.425337932547567 14.674012408127446 34 16.00048834086192 40.808204126480284 30.240507874496398 12.950799658161397 35 13.122188494312987 47.36373721488742 29.90771556400785 9.606358726791745 36 14.017106531473821 42.21970057469161 30.11213197164367 13.651060922190904 37 12.277853725222146 41.349721706864564 35.23337564690948 11.139048921003807 38 12.516015667899111 41.792046466791135 35.16857634440092 10.523361520908836 39 11.815653750505335 41.501182177902464 34.59554815077975 12.087615920812457 40 11.556911809581935 41.627097955447056 36.31858407079646 10.49740616417455 41 10.204056710913553 43.103469330435104 34.8899119539865 11.802562004664852 42 11.148599090509022 43.23993936726811 34.13036037357586 11.48110116864701 43 12.445145280966177 43.221240236165606 34.13888787023115 10.19472661263706 44 11.156994502138057 44.218692730604765 35.423335369578496 9.200977397678681 45 13.189674138900434 43.272545041190995 34.44153812305962 9.09624269684895 46 11.071175334360253 42.98760360889019 34.735568106799676 11.205652949949876 47 12.60880195599022 42.465770171149146 34.687041564792175 10.23838630806846 48 13.111928713742651 42.75097480778868 34.14333035899818 9.993766119470488 49 13.270570974446755 43.92590781269104 33.024819660105145 9.77870155275706 50 11.385387345490148 44.546567127646554 34.26141231183454 9.80663321502876 51 11.138153710679518 45.727948730492635 34.29259820948094 8.841299349346901 52 11.710234987583947 45.18342732021187 32.879912904133384 10.226424788070803 53 11.483072104149079 44.795604987213835 32.96381945209167 10.757503456545413 54 12.275657886688117 45.277040335702665 32.1046256988708 10.342676078738423 55 12.9777157751753 45.064062557362604 31.315393369800653 10.642828297661442 56 13.281278684110687 45.22023277729503 30.94028809555863 10.55820044303565 57 13.889398778711895 45.023679283380446 30.557901048741385 10.529020889166269 58 13.86851482238848 44.572471132770886 29.92395949404288 11.635054550797753 59 13.623394276337542 44.68350865162073 30.840915492095373 10.852181579946365 60 14.378116156852178 44.2429959206674 29.45939655278019 11.919491369700236 61 14.767581328176632 43.2318311996671 30.630553447727216 11.37003402442905 62 13.79626909518214 43.317910301605956 29.03569330199765 13.850127301214258 63 15.190911650568633 45.384210461885 26.943087639404066 12.4817902481423 64 15.301784861143439 45.668924795727115 26.868469086499005 12.160821256630447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.0 4 6.0 5 28.5 6 47.0 7 351.0 8 655.0 9 537.0 10 316.0 11 213.0 12 220.5 13 391.5 14 555.0 15 732.5 16 1092.5 17 1275.0 18 1558.0 19 1841.0 20 2114.0 21 2549.0 22 2711.0 23 3099.5 24 4431.0 25 5374.0 26 6556.5 27 5976.0 28 4213.0 29 4342.0 30 3752.5 31 3034.0 32 2922.5 33 2811.0 34 2754.0 35 2524.0 36 2351.0 37 2241.0 38 2062.0 39 1993.0 40 1944.5 41 1879.0 42 1862.0 43 1974.0 44 2086.0 45 2148.5 46 2014.0 47 1817.0 48 1788.5 49 1827.0 50 1894.0 51 1995.0 52 2435.0 53 2774.0 54 2790.0 55 2479.0 56 2152.0 57 1980.5 58 1809.0 59 1614.0 60 1385.0 61 1351.0 62 1121.0 63 714.0 64 537.0 65 482.0 66 349.0 67 271.0 68 233.0 69 195.0 70 170.5 71 123.5 72 101.0 73 86.5 74 55.5 75 39.0 76 33.5 77 21.0 78 14.0 79 10.5 80 7.0 81 7.0 82 5.5 83 4.0 84 3.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.06080431953886004 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08390996096362686 16 0.025537814206321216 17 0.13133733020393767 18 0.0 19 0.0 20 0.027969986987875617 21 0.0 22 0.0 23 0.0 24 0.0024321727815544013 25 0.07174909705585485 26 0.025537814206321216 27 0.19822208169668373 28 0.1459303668932641 29 0.12282472546849728 30 0.17633252666269414 31 0.22011163673067335 32 0.1507947124563729 33 0.22862424146611374 34 0.39036373143948144 35 0.24564945093699456 36 0.333207671072953 37 0.36847417640549185 38 0.34050418941761623 39 0.7320840072478749 40 0.37212243557782343 41 0.41590154564580273 42 0.5192688888618647 43 0.5156206296895331 44 0.4633289148861135 45 0.5071080249540927 46 0.526565407206528 47 0.5241332344249735 48 0.5107562841264244 49 0.5362940983327456 50 0.6335810095949216 51 0.5679123444929528 52 0.5861536403546108 53 0.6104753681701548 54 0.5983145042623828 55 0.6250684048594812 56 0.6347970959856988 57 0.6250684048594812 58 0.6858727243983412 59 0.6931692427430044 60 0.7296518344663205 61 0.6384453551580304 62 0.6506062190658024 63 0.6615509965827973 64 0.7296518344663205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 82231.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.79991730612543 #Duplication Level Percentage of deduplicated Percentage of total 1 84.63042931004435 53.14783962252678 2 8.212466838364865 10.314844766572218 3 3.07894889719409 5.800732084007247 4 1.4116690226757809 3.5461079155063175 5 0.697120505025077 2.1889555033989616 6 0.44925543657171624 1.6927922559618633 7 0.307894889719409 1.3535041529350245 8 0.24399217675877694 1.2258150819034186 9 0.1471698843941829 0.8318030912916055 >10 0.7203578551925796 7.6114847198744995 >50 0.06390271296063206 2.700927873916163 >100 0.03291957940396197 3.9693059794967835 >500 0.0 0.0 >1k 0.003872891694583761 5.615886952609114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3063 3.724872614950566 No Hit CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 1.891014337658547 No Hit CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 498 0.605611022607046 No Hit CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 382 0.46454500127689075 No Hit CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 272 0.33077549829139863 No Hit CAAAGTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 214 0.26024248762632096 No Hit CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.2565942284539894 No Hit CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.21767946394911894 No Hit CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC 174 0.21159903199523292 Illumina Single End Adapter 2 (96% over 31bp) CAAAGTGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTAGTGGTCCA 165 0.20065425447823812 No Hit CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.19822208169668373 No Hit CAAAGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 163 0.19822208169668373 No Hit CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136 0.16538774914569931 No Hit CAAAGTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.1617394899733677 No Hit CAAAGTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131 0.15930731719181332 No Hit CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.1580912308010361 No Hit CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA 105 0.1276890710316061 No Hit CAAAGTGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.12647298464082887 No Hit CAAAGTGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.12647298464082887 No Hit CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG 98 0.11917646629616568 No Hit CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.11552820712383409 No Hit CAAAGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 94 0.11431212073305687 No Hit CAAAGTGGGGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCTTTTCAT 90 0.10944777516994808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0012160863907772009 0.0 0.0 0.0 0.0 8 0.006080431953886004 0.0 0.0 0.0 0.0 9 0.006080431953886004 0.0 0.0 0.0 0.0 10 0.05715606036652844 0.0 0.0 0.0 0.0 11 0.10823168877917087 0.0 0.0 0.0 0.0 12 0.11187994795150248 0.0 0.0 0.0 0.0 13 0.11431212073305688 0.0 0.0 0.0 0.0 14 0.1507947124563729 0.0 0.0 0.0 0.0 15 0.1678199219272537 0.0 0.0 0.0 0.0 16 0.17511644027191692 0.0 0.0 0.0 0.0 17 0.18362904500735733 0.0 0.0 0.0 0.0 18 0.19335773613357493 0.0 0.0 0.0 0.0 19 0.20551860004134695 0.0 0.0 0.0 0.0 20 0.24929771010932616 0.0 0.0 0.0 0.0 21 0.2736194379248702 0.0 0.0 0.0 0.0 22 0.36725809001471466 0.0 0.0 0.0 0.0 23 0.40982111369191665 0.0 0.0 0.0 0.0 24 0.4450876190244555 0.0 0.0 0.0 0.0 25 0.4560323965414503 0.0 0.0 0.0 0.0 26 0.4657610876676679 0.0 0.0 0.0 0.0 27 0.4815702107477715 0.0 0.0 0.0 0.0 28 0.5922340723084968 0.0 0.0 0.0 0.0 29 0.6080431953886004 0.0 0.0 0.0 0.0 30 0.6688475149274604 0.0 0.0 0.0 0.0 31 0.6883048971798956 0.0 0.0 0.0 0.0 32 0.7041140202599993 0.0 0.0 0.0 0.0 33 0.7138427113862169 0.0 0.0 0.0 0.0 34 0.727219661684766 0.0 0.0 0.0 0.0 35 0.8695017694056986 0.0 0.0 0.0 0.0 36 0.8865269788765794 0.0 0.0 0.0 0.0 37 0.9108487066921234 0.0 0.0 0.0 0.0 38 0.9315221753353358 0.0 0.0 0.0 0.0 39 0.9534117303693255 0.0 0.0 0.0 0.0 40 0.9825978037479782 0.0 0.0 0.0 0.0 41 1.0275930002067346 0.0 0.0 0.0 0.0 42 1.065291678320828 0.0 0.0 0.0 0.0 43 1.089613406136372 0.0 0.0 0.0 0.0 44 1.1285281706412424 0.0 0.0 0.0 0.0 45 1.1735233670999987 0.0 0.0 0.0 0.0 46 1.2100059588233147 0.0 0.0 0.0 0.0 47 1.241624204983522 0.0 0.0 0.0 0.0 48 1.2793228830976153 0.0 0.0 0.0 0.0 49 1.314589388430154 0.0 0.0 0.0 0.0 50 1.3401272026364752 0.0 0.0 0.0 0.0 51 1.3583684984981332 0.0 0.0 0.0 0.0 52 1.3729615351874598 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCTAC 25 8.203477E-6 58.46083 34 TTCGCTA 30 3.52009E-7 58.460823 33 ACCCGCT 15 0.0045274654 58.460823 33 TTCTATG 15 0.0045274654 58.460823 34 TAGAGCG 15 0.0045274654 58.460823 34 CGCTGAA 15 0.0045496416 58.388786 36 CTATGTG 15 0.0045496416 58.388786 36 TCTATGT 15 0.0045607598 58.352833 35 TTACCCG 15 0.0045830575 58.281055 31 TATGTGC 15 0.0045830575 58.281055 37 ACCGTAG 20 1.9520246E-4 58.281055 39 ATGTGCT 15 0.0045830575 58.281055 38 CTGAATT 15 0.0045830575 58.281055 38 AACCAAT 15 0.0045830575 58.281055 31 TACCGTA 20 1.9520246E-4 58.281055 38 GCTGAAT 15 0.0045830575 58.281055 37 GTAATTC 15 0.0045830575 58.281055 30 GTTACCC 15 0.0045830575 58.281055 30 TGTGCTA 15 0.0045830575 58.281055 39 CTACCGT 20 1.9520246E-4 58.281055 37 >>END_MODULE