FastQCFastQC Report
Fri 17 Jun 2016
SRR1526623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70074
Sequences flagged as poor quality0
Sequence length56
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA42076.003653280817422No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT20122.8712503924422754No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.709250221194737No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.6535947712418301No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.5380026828781003No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.35533864200702114No Hit
CAAAGTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.3353597625367469No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1860.26543368439078685No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.25544424465564974No Hit
CAAAGTGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTA1780.2540171818363444No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA1740.2483089305591232No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1700.24260067928190196No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.24117361646259666No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.22262179981162772No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.1926534806062163No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1250.17838285241316323Illumina Single End Adapter 2 (95% over 23bp)
CAAAGTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.1741016639552473No Hit
CAAAGTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.1712475383166367No Hit
CAAAGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.15412278448497305No Hit
CAAAGTGGGTGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA1030.1469874703884465No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAG1000.1427062819305306No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.1427062819305306No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCTAAAAAAAAAAAAAAAAAAAAAAAAAA980.13985215629191997No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAA970.13842509347261467No Hit
CAAAGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA960.13699803065330937No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAA940.13414390501469875No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1241544652795616No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA870.1241544652795616No Hit
CAAAGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG860.1227274024602563No Hit
CAAAGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.12130033964095098No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA840.1198732768216457No Hit
CAAAGTGGGGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATC840.1198732768216457No Hit
CAAAGTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.11844621400234037No Hit
CAAAGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC810.11559208836372976No Hit
CAAAGTGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.11416502554442447No Hit
CAAAGTGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA770.10988383708650856No Hit
CAAAGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.10702971144789794No Hit
CAAAGTGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.10132146017067672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA203.8940817E-450.66472637
GAGCACA203.9221518E-450.5911436
CAGAGCA251.936391E-550.5544334
GAGCGGA150.00803945750.55442434
TTCTATG150.00803945750.55442434
TGCGATC150.00813219550.40810432
ATTCTAT150.00813219550.40810433
GTCGATC150.00815550350.37165531
ATGGGAT150.00822572650.26262739
TCTAGTG150.00822572650.26262748
TTTCTAG150.00822572650.26262746
TTCTAGT150.00822572650.26262747
ATTTCTA150.00822572650.26262745
TGTGCTA150.00822572650.26262739
TAGTGGG150.008249233550.22638750
TATTTCT150.008249233550.22638744
CTATTTC150.008249233550.22638743
GCTATTT150.0082727950.190242
CTAGTGG150.0082727950.190249
CAAGTGG150.0082727950.190242
Produced by FastQC (version 0.11.5)