FastQCFastQC Report
Fri 17 Jun 2016
SRR1526622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13771
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2221.6120833635901535No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1841.3361411662188656No Hit
CGAGTAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA1461.0601989688475784No Hit
CGAGTAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG850.6172391256989326No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.5954542153801466No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.493791300559146No Hit
CGAGTAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG610.44295984314864567No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG530.3848667489652167No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG470.3412969283276451No Hit
CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.2977271076900733No Hit
CGAGTAGGGATATTGATATGCCGAGAAGCACTAACATTTTTGATTGGGTCCAGCTGCCCATTGT380.2759421973712875No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.2686805605983589No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.246895650279573No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.2323723767337158No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.21784910318785855No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG250.18154091932321545No Hit
CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.17427928255028682No Hit
CGAGTAGGGGAAGAAAACATTGATTATGACTACATAGCAACCTTATGTGAAGGTTTTAGTGGTT240.17427928255028682No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.1670176457773582No Hit
CGAGTACCAATAAAGACATCCGAGTGTCCTGCTGCTCCTCTGAAAAAAAAAAAAAAAAAAAAAA220.1597560090044296No Hit
CGAGTAGGAAGCATGTATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1597560090044296No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA220.1597560090044296No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG210.15249437223150097No Hit
CGAGTAGGGATTTAATTTATTTCTCTGATGAACCTTTTTATAATATCTCAAGTTTAATTTCAAA190.13797109868564375No Hit
CGAGTAGGGACTGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.13070946191271512No Hit
CGAGTAGGGAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1234478251397865No Hit
CGAGTAGGGATATTCAGGGATGTTTCTTTTTATGCTACTTGTATCTGATCCTCTTCCAACTCAG160.1161861883668579No Hit
CGAGTAGGGAGCAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1161861883668579No Hit
CGAGTAGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1161861883668579No Hit
CGAGTAGGGACATGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1161861883668579No Hit
CGAGTAGGGTTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10892455159392928No Hit
CGAGTAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10892455159392928No Hit
CGAGTAGGGAAGCAGTGGTACAACGAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10892455159392928No Hit
CGAGTAGGGAGCCTCTTCCCTCCTTCACATCCGTTCCCCATCTCATACCACCGTGCATGCTTTT150.10892455159392928No Hit
CGAGTAGGGAAGCATGGTATCAACGCAGAGTCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10166291482100065No Hit
CGAGTAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10166291482100065No Hit
CGAGTAGGGATGGTACACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10166291482100065No Hit
CGAGTAGGGATTGTTTTTTTTTTTATTTATATTACTATTGTTTTGATGTGTCATTTGAGTAGCA140.10166291482100065No Hit
CGAGTAAGGACTCGAGTAGGGAAGCATGGTCAAAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT140.10166291482100065No Hit
CGAGTAGGGGAGTTGATTATGATGTAAGCGGAACGCGGCTGGGAAGCCGGACCGTAGGAGGTGA140.10166291482100065No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA140.10166291482100065No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10166291482100065No Hit
CGAGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10166291482100065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATG150.00458954958.0000048
GGAGAGT303.4940786E-758.0000048
GGGCATT150.00458954958.0000047
CTCTTTT150.00458954958.00000429
AGGGATC150.00458954958.0000046
ACTCAGT150.00458954958.00000434
CTCAGTA150.00458954958.00000435
CCCTGCG150.00458954958.00000419
GGAAGTA150.00458954958.0000048
GCATGGT303.4940786E-758.00000412
GGAAGAT150.00458954958.0000048
GGGATAT150.00458954958.0000047
AGCATGT150.00458954958.00000411
AGCATGG303.4940786E-758.00000411
GAGAGTG150.00458954958.0000049
GGGAATT150.00458954958.00000410
GGGGTCG150.00458954958.0000047
GGGAAGT303.4940786E-758.0000047
GGAATTC150.00458954958.00000411
GGGTGTA150.00458954958.0000047
Produced by FastQC (version 0.11.5)