##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526622_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13771 Sequences flagged as poor quality 0 Sequence length 64 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.78113426766393 38.0 38.0 40.0 34.0 40.0 2 37.376951564882724 38.0 38.0 40.0 33.0 40.0 3 36.30985404110086 38.0 35.0 40.0 30.0 40.0 4 37.32568440926585 38.0 38.0 40.0 33.0 40.0 5 35.68636990777721 38.0 35.0 39.0 29.0 40.0 6 37.811124827536126 39.0 38.0 40.0 35.0 40.0 7 37.89724783966306 39.0 38.0 40.0 35.0 40.0 8 37.90095127441725 39.0 38.0 40.0 35.0 40.0 9 37.855929126425096 39.0 38.0 40.0 35.0 40.0 10 36.93399172173408 38.0 36.0 40.0 32.0 40.0 11 37.59269479340644 39.0 38.0 40.0 33.0 40.0 12 37.466342313557476 39.0 37.0 40.0 33.0 40.0 13 37.33948151913441 39.0 37.0 40.0 33.0 40.0 14 37.403166073632995 39.0 37.0 40.0 33.0 40.0 15 37.42320819112628 39.0 37.0 40.0 33.0 40.0 16 37.18393725945828 39.0 36.0 40.0 33.0 40.0 17 37.13542952581512 39.0 36.0 40.0 33.0 40.0 18 37.10297000944013 39.0 36.0 40.0 33.0 40.0 19 36.91104494953162 39.0 36.0 40.0 32.0 40.0 20 37.06927601481374 39.0 36.0 40.0 33.0 40.0 21 36.90821291119018 39.0 36.0 40.0 33.0 40.0 22 36.63009222278701 38.0 36.0 40.0 31.0 40.0 23 36.51463219809745 38.0 36.0 40.0 31.0 40.0 24 36.241231573596686 38.0 35.0 40.0 30.0 40.0 25 36.38370488708155 38.0 35.0 40.0 31.0 40.0 26 36.70909883087648 39.0 36.0 40.0 32.0 40.0 27 36.85955994481156 39.0 36.0 40.0 32.0 40.0 28 36.70953452908285 39.0 36.0 40.0 32.0 40.0 29 36.61535110013797 39.0 36.0 40.0 31.0 40.0 30 36.49030571490814 39.0 36.0 40.0 31.0 40.0 31 36.6340135066444 39.0 36.0 40.0 32.0 40.0 32 36.3677292861811 39.0 36.0 40.0 31.0 40.0 33 36.502868346525304 39.0 36.0 40.0 31.0 40.0 34 36.47788831602643 39.0 36.0 40.0 31.0 40.0 35 36.2774671410936 39.0 35.0 40.0 31.0 40.0 36 35.99934645269044 39.0 35.0 40.0 31.0 40.0 37 35.78643526250817 39.0 35.0 40.0 30.0 40.0 38 35.59371142255464 39.0 35.0 40.0 29.0 40.0 39 35.618764069421246 39.0 35.0 40.0 29.0 40.0 40 35.41391329605693 39.0 35.0 40.0 29.0 40.0 41 34.97342240941108 39.0 35.0 40.0 27.0 40.0 42 34.99201219954978 39.0 35.0 40.0 27.0 40.0 43 34.82572071744971 39.0 35.0 40.0 27.0 40.0 44 34.51746423643889 39.0 34.0 40.0 25.0 40.0 45 34.45893544404909 39.0 35.0 40.0 25.0 40.0 46 34.13797109868565 38.0 35.0 40.0 22.0 40.0 47 33.967177401786365 38.0 34.0 40.0 21.0 40.0 48 33.87372013651877 38.0 34.0 40.0 20.0 40.0 49 33.47265993754992 38.0 33.0 39.0 16.0 40.0 50 33.24929199041464 38.0 33.0 39.0 12.0 40.0 51 32.591532931522764 38.0 33.0 39.0 4.0 40.0 52 32.44295984314864 37.0 32.0 39.0 4.0 40.0 53 32.006680705831094 37.0 32.0 39.0 4.0 40.0 54 31.643235785346018 37.0 32.0 39.0 4.0 40.0 55 31.0723985186261 36.0 31.0 39.0 4.0 40.0 56 30.089245515939293 36.0 29.0 39.0 4.0 40.0 57 29.609469174351897 35.0 28.0 39.0 4.0 40.0 58 29.269769806114297 35.0 26.0 39.0 4.0 40.0 59 28.804081039866386 35.0 24.0 39.0 4.0 40.0 60 28.28407523055697 35.0 21.0 39.0 4.0 40.0 61 27.69791591024617 35.0 4.0 39.0 4.0 40.0 62 27.610776268971026 35.0 4.0 39.0 4.0 40.0 63 27.123883523346162 35.0 4.0 39.0 4.0 40.0 64 26.909156923970663 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.28737460018974303 1 2 -1.030719070845123 1 3 -1.0655353050311263 1 4 -1.3598816216463234 1 5 -0.5832095075792623 1 6 -0.7614880684208387 1 7 -0.9827471314866472 1 8 -0.6306265163408042 1 9 -0.5452028830180069 1 10 -0.6480148469644291 1 11 0.023316319534828267 1 12 -0.9741797651461539 1 13 -1.3305874564277929 1 14 0.3597625530398787 1 15 -0.8279805395351545 1 16 0.6230494762007197 1 17 0.008789436520530103 1 18 1.0981771030090357 1 19 1.0506938672905122 1 20 0.03487800714692213 1 21 -0.23310368436418116 1 22 0.6982535438417514 1 23 -0.3997719134273723 1 24 0.8361720212560613 1 25 -1.3146081276333348 1 26 -2.353843215607924 1 27 -2.131317305476955 1 28 -2.4290285811294012 1 29 -0.71645833158437 1 30 -0.5621895611391281 1 31 -0.2564600665441077 1 32 -0.6665354953170066 1 33 0.19459727947121763 1 34 -1.6623161852153387 1 35 -4.099218172117325 1 36 -5.0689127632404976 1 37 -4.469588441437185 1 38 -6.08651668126458 1 39 -4.5985018249723915 1 40 -4.205658020784078 1 41 -5.646193978757015 1 42 -5.322701015137991 1 43 -5.9793655224327615 1 44 -3.6030646990730943 1 45 -7.983056299442861 1 46 -4.073686887342337 1 47 -5.300242311376774 1 48 -4.270934037950834 1 49 -8.100686405098163 1 50 -6.510913502930304 1 51 -7.94772878848509 1 52 -8.518424269474696 1 53 -7.897845478517741 1 54 -7.0351887141803005 1 55 -6.9779008806319744 1 56 -6.081463074740384 1 57 -5.1819967614085165 1 58 -3.7862744165265347 1 59 -6.009487664529679 1 60 -5.624264437709812 1 61 -6.439652737551892 1 62 -4.449981064686945 1 63 -4.915076496799191 1 64 -4.850686894804529 2 1 -0.37070793352307163 2 2 -0.36405240417845874 2 3 -0.5655353050311263 2 4 -0.026548288312994828 2 5 -0.9998761742459266 2 6 -0.09482140175417442 2 7 0.10058620184668143 2 8 -0.21395984967413995 2 9 -0.6285362163513426 2 10 -0.3146815136310934 2 11 -2.0600170137985074 2 12 -1.974179765146154 2 13 -0.9972541230944572 2 14 -0.723570780293457 2 15 0.1720194604648455 2 16 -0.04361719046594459 2 17 -0.9912105634794699 2 18 -0.0684895636576357 2 19 1.1340272006238408 2 20 -0.13178865951974217 2 21 -2.566437017697517 2 22 -0.3850797894915843 2 23 -1.6497719134273723 2 24 -1.330494645410603 2 25 -1.731274794299999 2 26 -0.10384321560792387 2 27 0.2853493611897093 2 28 0.32097141887059877 2 29 0.2002083350823014 2 30 1.187810438860872 2 31 0.7435399334558923 2 32 0.916797838016322 2 33 -0.47206938719544667 2 34 -0.6623161852153387 2 35 1.317448494549339 2 36 0.5144205700928381 2 37 0.7804115585628182 2 38 0.5801499854020875 2 39 -0.1818351583057236 2 40 1.5443419792159219 2 41 1.1038060212429812 2 42 1.593965651528677 2 43 1.6873011442339063 2 44 1.3969353009269057 2 45 1.600277033890471 2 46 2.092979779324331 2 47 2.866424355289894 2 48 3.7290659620491624 2 49 2.3159802615685052 2 50 2.7390864970696924 2 51 2.7189378781815776 2 52 4.314909063858636 2 53 4.268821188148923 2 54 2.381477952486364 2 55 3.3554324527013577 2 56 -0.33146307474038395 2 57 -2.265330094741852 2 58 -1.2862744165265347 2 59 -0.9261543311963472 2 60 -2.6242644377098117 2 61 -0.022986070885224308 2 62 -2.3666477313536127 2 63 -3.0817431634658554 2 64 -1.9340202281378644 3 1 0.06518950237435917 3 2 -0.8961036862297362 3 3 -0.30912504862087076 3 4 -0.27013803190273933 3 5 -0.5062864306561821 3 6 -0.22302652995929861 3 7 0.26084261210309734 3 8 0.42706579135150236 3 9 0.1278740400589129 3 10 0.2622415632919868 3 11 0.21562401184252167 3 12 -0.012641303607693999 3 13 -1.0998182256585594 3 14 -0.5440836008062746 3 15 -2.0266984882531034 3 16 -0.1974633443120979 3 17 -0.52967210194101 3 18 0.3994591542910868 3 19 1.2942836108802567 3 20 -0.03563481336589547 3 21 1.4143322130717166 3 22 1.0636381592263646 3 23 0.1322793686239052 3 24 1.2977104827945212 3 25 1.2238534108282053 3 26 -1.443586805351508 3 27 -1.2467019208615682 3 28 -0.8200542221550435 3 29 -3.0754326905587277 3 30 -1.0878305867801572 3 31 -0.423126733210772 3 32 -0.4037149824964956 3 33 -0.5297616948877533 3 34 -1.476418749317908 3 35 -0.5607566336557852 3 36 0.10416415983642224 3 37 -0.3990756209243642 3 38 -0.7916448863927883 3 39 0.1835494570788896 3 40 -0.12873494386099793 3 41 -0.02439910696214298 3 42 0.9593502669132903 3 43 0.5142242211569865 3 44 0.8200122240038255 3 45 1.08745652106996 3 46 1.0352874716320244 3 47 1.6100140988796383 3 48 1.3316300646132646 3 49 -0.9404299948417503 3 50 0.39934290732610833 3 51 0.1932968525405485 3 52 1.5264475253970957 3 53 1.7367699060976456 3 54 2.272503593512006 3 55 1.9772273244962335 3 56 1.9569984637211562 3 57 2.055182725770969 3 58 3.22654609629398 3 59 3.5161533611113462 3 60 -0.09862341206878611 3 61 -0.9076014555006076 3 62 -1.1422887569946383 3 63 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1.5216147064886485 4 41 2.437139354576317 4 42 2.162147469710497 4 43 1.5282102351429998 4 44 1.9878443918359991 4 45 0.38815582176926 4 46 -0.11156567522112226 4 47 0.4421819310474646 4 48 1.4714902044734046 4 49 1.891737837326076 4 50 2.5951471031303015 4 51 2.067422726666422 4 52 0.06490906385863582 4 53 0.9885181578458919 4 54 0.6920840130924262 4 55 -0.83396148669258 4 56 -2.7632812565585674 4 57 -1.644117973529731 4 58 -1.8014259316780503 4 59 -1.1761543311963472 4 60 -1.3363856498310263 4 61 -0.25025879815795093 4 62 0.0045643898585083775 4 63 0.5470447153220235 4 64 0.6871918930742567 5 1 0.21903565622051246 5 2 -1.0499498400758895 5 3 0.07549033599451604 5 4 0.2683235065588008 5 5 0.7244828001130443 5 6 0.23851193157916128 5 7 0.26084261210309734 5 8 0.35014271442842926 5 9 0.1278740400589129 5 10 0.03147233252275328 5 11 -1.3228375266190184 5 12 -1.551102842069234 5 13 -1.7921259179662528 5 14 -1.313314370037041 5 15 -0.7190061805607968 5 16 -0.9666941135428644 5 17 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1.1389658660667124 6 35 2.054627981728828 6 36 -2.8958358401635778 6 37 -1.3990756209243642 6 38 -4.1762602710081715 6 39 -2.20106592753649 6 40 -2.590273405399465 6 41 -5.024399106962143 6 42 -4.9637266561636295 6 43 -4.716545009612247 6 44 -4.6415262375346344 6 45 -6.14331270969927 6 46 -6.503174066829516 6 47 -7.159216670351135 6 48 -5.899139166155962 6 49 -6.325045379457137 6 50 -8.062195554212355 6 51 -7.883626224382528 6 52 -7.242783243833671 6 53 -7.417076247748511 6 54 -6.727496406487994 6 55 -6.561234213965307 6 56 -6.966078459355767 6 57 -7.252509581921338 6 58 -6.77345390370602 6 59 -6.483846638888654 6 60 -5.944777258222633 6 61 -6.676832224731378 6 62 -5.834596449302328 6 63 -6.3830252147479065 6 64 -8.382738176855813 7 1 -0.16557972839486723 7 2 -0.43456522469127634 7 3 -0.8475865870824038 7 4 0.4221696604049541 7 5 0.9552520308822778 7 6 -0.7614880684208387 7 7 -0.35454200328152297 7 8 -0.26524190095619105 7 9 -0.1028951907103135 7 10 0.3391646402150599 7 11 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-4.323570780293451 119 15 -2.744647206201826 119 16 -3.3769505237992803 119 17 -1.99121056347947 119 18 -1.9851562303243 119 19 -3.7159727993761535 119 20 -3.2817886595197407 119 21 -3.683103684364184 119 22 -4.551746456158249 119 23 -3.283105246760705 119 24 -3.3638279787439416 119 25 -3.914608127633336 119 26 -2.587176548941258 119 27 -1.9646506388102907 119 28 -3.445695247796067 119 29 -2.9831249982510357 119 30 -2.9288562278057952 119 31 -2.6897933998774377 119 32 -4.0498688286503395 119 33 -2.6220693871954452 119 34 -5.778982851882009 119 35 -4.56588483878399 119 36 -9.15224609657383 119 37 -8.352921774770518 119 38 -6.719850014597913 119 39 -8.365168491639057 119 40 -8.338991354117411 119 41 -7.096193978757018 119 42 -7.789367681804656 119 43 -8.696032189099427 119 44 -8.303064699073094 119 45 -7.733056299442861 119 46 -8.523686887342336 119 47 -9.13357564471011 119 48 -10.370934037950835 119 49 -8.884019738431498 119 50 -9.544246836263639 119 51 -10.047728788485092 119 52 -9.00175760280803 119 53 -10.314512145184409 119 54 -11.168522047513633 119 55 -7.2279008806319744 119 56 -6.314796408073718 119 57 -6.165330094741851 119 58 -7.486274416526534 119 59 -7.776154331196349 119 60 -6.857597771043146 119 61 -8.256319404218559 119 62 -7.583314398020278 119 63 -7.465076496799188 119 64 -7.834020228137863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 34.0 5 1.0 6 7.0 7 8.0 8 15.0 9 10.0 10 12.0 11 15.0 12 29.0 13 18.0 14 26.0 15 38.0 16 42.0 17 55.0 18 54.0 19 51.0 20 58.0 21 82.0 22 95.0 23 113.0 24 128.0 25 158.0 26 165.0 27 202.0 28 249.0 29 306.0 30 387.0 31 510.0 32 595.0 33 730.0 34 885.0 35 946.0 36 1169.0 37 1695.0 38 2291.0 39 2584.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 98.40970154672863 1.1909084307602933 0.0798780045022148 0.3195120180088592 8 99.2157432285237 0.37760511219228815 0.07261636772928619 0.3340352915547164 9 97.48021203979377 1.713746278411154 0.3267736547817878 0.4792680270132888 10 40.21494444847868 38.16716287851282 8.183864643090551 13.434028029917943 11 22.140730520659357 32.699150388497564 29.213564737491833 15.946554353351244 12 51.86260983225619 18.669668143199477 17.6603006317624 11.807421392781933 13 23.70198242683901 19.38130854694648 29.249872921356474 27.666836104858035 14 28.516447607290687 30.79660155399027 22.227870161934497 18.45908067678455 15 35.41500254157287 23.854476799070508 20.80458935444049 19.925931304916126 16 22.866894197952217 25.945828189673954 28.05896449059618 23.128313121777648 17 28.37847650860504 26.439619490233095 18.125045385229832 27.056858615932033 18 24.22482027448987 31.805969065427348 21.22576428727035 22.743446372812432 19 21.70503231428364 25.953089826446877 31.907631980248347 20.43424587902113 20 20.717449713165347 29.286181105221115 19.896884757824413 30.099484423789125 21 27.296492629438674 26.14915401931595 23.854476799070508 22.69987655217486 22 23.202614379084967 34.16122004357299 18.395061728395063 24.241103848946985 23 34.863118146830296 30.564229177256554 18.851209062522692 15.72144361339046 24 22.54901960784314 35.66448801742919 24.12490922294844 17.66158315177923 25 24.794828963613917 30.096593797661413 26.30546880673978 18.803108431984896 26 26.311582618805406 25.490481034733325 27.241679988373786 20.956256358087487 27 23.653412808025006 25.27440575706913 27.098931453078436 23.973249981827433 28 25.110836543353443 28.446834799040627 27.545606512101173 18.89672214550476 29 23.94417387511812 26.909936759467907 31.220469579123357 17.925419786290618 30 17.46020207894163 32.66700588791161 34.86952097114197 15.003271062004798 31 18.61547411285631 30.373763816172193 36.75828970331588 14.252472367655614 32 12.187318208260617 31.559045956951714 44.153577661431065 12.100058173356603 33 13.393441430960518 31.76034319784774 39.42412564531375 15.42208972587799 34 14.290909090909091 30.661818181818184 42.56727272727272 12.479999999999999 35 12.548192332872626 33.01083872844984 42.052811522514006 12.388157416163526 36 13.0412633723892 30.66734589913398 42.47871333964049 13.812677388836327 37 12.124299541518084 30.04148169711084 45.084055017829854 12.750163743541226 38 10.262008733624455 29.934497816593886 47.88209606986899 11.921397379912664 39 12.994103516051538 30.552522384800174 44.87879449661498 11.574579602533303 40 13.32799534138885 32.69762701994468 44.55524821662542 9.419129422041054 41 11.02855477855478 32.55390442890443 45.91346153846153 10.504079254079254 42 14.174318612447165 29.507360443084096 45.03716659379099 11.281154350677744 43 12.618457501093456 31.892404140545267 45.28356903338679 10.205569324974487 44 10.761384335154826 30.67395264116576 48.93260473588342 9.632058287795992 45 12.468991682474828 32.372683496279 45.38888078213921 9.76944403910696 46 12.210234728094473 33.53987461729115 44.022452252514945 10.22743840209943 47 11.53229333722117 34.99052339991252 44.190115177139525 9.287068085726782 48 14.50765864332604 32.93216630196937 43.27498176513494 9.285193289569657 49 10.1356278255797 34.04550094793641 45.49365611783579 10.325215108648097 50 11.167031363967906 35.032822757111596 43.413566739606125 10.38657913931437 51 9.9613505432801 33.13643987457158 45.4750966236418 11.427112958506527 52 11.358493320680342 36.21432221330024 41.89356887364041 10.533615592379006 53 11.702438312162359 34.961308220178125 43.34939407212732 9.986859395532194 54 11.368282710280374 35.908294392523366 41.73481308411215 10.988609813084112 55 12.00087597634864 36.593911964376964 40.492006715818675 10.913205343455727 56 11.425025551175354 38.28296101620675 38.991093590305155 11.300919842312746 57 11.263595882911162 37.29469304328783 39.637929775896055 11.803781297904957 58 12.400496604104287 37.6688819104652 36.471189658949825 13.459431826480683 59 13.081586443649112 38.287926374990874 37.3310934190344 11.299393762325616 60 15.508177570093459 36.60192757009346 35.23656542056075 12.653329439252337 61 13.153666374525269 38.022202746129125 36.39351446099912 12.43061641834648 62 14.85647505660653 36.556862172229934 35.16908918267475 13.417573588488787 63 16.26734842951059 38.59751643535427 33.929875821767716 11.20525931336742 64 16.26123164584703 39.4404266199138 33.05573818394331 11.242603550295858 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.5 8 5.0 9 30.0 10 87.0 11 119.0 12 103.0 13 90.5 14 94.0 15 116.5 16 193.5 17 248.0 18 238.0 19 228.0 20 279.0 21 369.5 22 409.0 23 494.0 24 631.5 25 684.0 26 751.0 27 876.5 28 935.0 29 788.0 30 633.5 31 626.0 32 535.0 33 444.0 34 464.5 35 458.0 36 431.0 37 423.0 38 405.0 39 395.0 40 398.5 41 402.5 42 403.0 43 361.5 44 320.0 45 336.0 46 387.0 47 422.0 48 365.5 49 318.0 50 327.0 51 299.5 52 266.5 53 261.0 54 319.5 55 495.0 56 612.0 57 482.0 58 352.0 59 308.0 60 257.5 61 251.0 62 225.0 63 175.5 64 152.0 65 132.0 66 91.5 67 71.0 68 61.5 69 52.0 70 44.0 71 26.0 72 16.0 73 13.0 74 12.5 75 15.0 76 9.5 77 4.5 78 5.0 79 4.0 80 3.5 81 4.0 82 2.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.007261636772928619 23 0.0 24 0.007261636772928619 25 0.014523273545857238 26 0.06535473095635756 27 0.10166291482100065 28 0.08713964127514341 29 0.10166291482100065 30 0.10166291482100065 31 0.13797109868564375 32 0.13797109868564375 33 0.13070946191271512 34 0.15249437223150097 35 0.17427928255028682 36 0.21784910318785855 37 0.21784910318785855 38 0.22511073996078715 39 0.246895650279573 40 0.2396340135066444 41 0.3122503812359306 42 0.3558202018735023 43 0.3848667489652167 44 0.3340352915547164 45 0.4720063902403602 46 0.3848667489652167 47 0.3848667489652167 48 0.44295984314864567 49 0.4139132960569313 50 0.44295984314864567 51 0.42117493282985985 52 0.5228378476508605 53 0.5300994844237892 54 0.5446227579696463 55 0.5228378476508605 56 0.5300994844237892 57 0.5228378476508605 58 0.5664076682884323 59 0.5809309418342895 60 0.5446227579696463 61 0.5736693050613608 62 0.5809309418342895 63 0.5881925786072181 64 0.5954542153801466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 13771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.94575557330622 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12318286151492 43.918379202672284 2 10.5330272889569 11.996223948878077 3 4.8074470798265745 8.212911190182268 4 2.486610558530987 5.664076682884322 5 1.4154552410099464 4.030208408975383 6 0.9818923743942871 3.3548761890930217 7 0.5993369038510584 2.3890784982935154 8 0.408059168579444 1.8589790138697264 9 0.306044376434583 1.5685135429525814 >10 1.2369293547564397 10.46401858979014 >50 0.06375924509053812 2.5343112337520877 >100 0.03825554705432287 4.008423498656597 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222 1.6120833635901535 No Hit CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 1.3361411662188656 No Hit CGAGTAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA 146 1.0601989688475784 No Hit CGAGTAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG 85 0.6172391256989326 No Hit CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.5954542153801466 No Hit CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.493791300559146 No Hit CGAGTAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG 61 0.44295984314864567 No Hit CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 53 0.3848667489652167 No Hit CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG 47 0.3412969283276451 No Hit CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41 0.2977271076900733 No Hit CGAGTAGGGATATTGATATGCCGAGAAGCACTAACATTTTTGATTGGGTCCAGCTGCCCATTGT 38 0.2759421973712875 No Hit CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.2686805605983589 No Hit CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.246895650279573 No Hit CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.2323723767337158 No Hit CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.21784910318785855 No Hit CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG 25 0.18154091932321545 No Hit CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.17427928255028682 No Hit CGAGTAGGGGAAGAAAACATTGATTATGACTACATAGCAACCTTATGTGAAGGTTTTAGTGGTT 24 0.17427928255028682 No Hit CGAGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.1670176457773582 No Hit CGAGTACCAATAAAGACATCCGAGTGTCCTGCTGCTCCTCTGAAAAAAAAAAAAAAAAAAAAAA 22 0.1597560090044296 No Hit CGAGTAGGAAGCATGTATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.1597560090044296 No Hit CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA 22 0.1597560090044296 No Hit CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG 21 0.15249437223150097 No Hit CGAGTAGGGATTTAATTTATTTCTCTGATGAACCTTTTTATAATATCTCAAGTTTAATTTCAAA 19 0.13797109868564375 No Hit CGAGTAGGGACTGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.13070946191271512 No Hit CGAGTAGGGAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.1234478251397865 No Hit CGAGTAGGGATATTCAGGGATGTTTCTTTTTATGCTACTTGTATCTGATCCTCTTCCAACTCAG 16 0.1161861883668579 No Hit CGAGTAGGGAGCAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.1161861883668579 No Hit CGAGTAGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.1161861883668579 No Hit CGAGTAGGGACATGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.1161861883668579 No Hit CGAGTAGGGTTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.10892455159392928 No Hit CGAGTAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.10892455159392928 No Hit CGAGTAGGGAAGCAGTGGTACAACGAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.10892455159392928 No Hit CGAGTAGGGAGCCTCTTCCCTCCTTCACATCCGTTCCCCATCTCATACCACCGTGCATGCTTTT 15 0.10892455159392928 No Hit CGAGTAGGGAAGCATGGTATCAACGCAGAGTCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.10166291482100065 No Hit CGAGTAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.10166291482100065 No Hit CGAGTAGGGATGGTACACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.10166291482100065 No Hit CGAGTAGGGATTGTTTTTTTTTTTATTTATATTACTATTGTTTTGATGTGTCATTTGAGTAGCA 14 0.10166291482100065 No Hit CGAGTAAGGACTCGAGTAGGGAAGCATGGTCAAAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 14 0.10166291482100065 No Hit CGAGTAGGGGAGTTGATTATGATGTAAGCGGAACGCGGCTGGGAAGCCGGACCGTAGGAGGTGA 14 0.10166291482100065 No Hit CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA 14 0.10166291482100065 No Hit CGAGTAGGGGTGCAGTGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.10166291482100065 No Hit CGAGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.10166291482100065 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.014523273545857236 0.0 0.0 0.0 0.0 7 0.014523273545857236 0.0 0.0 0.0 0.0 8 0.014523273545857236 0.0 0.0 0.0 0.0 9 0.021784910318785853 0.0 0.0 0.0 0.0 10 0.050831457410500325 0.0 0.0 0.0 0.0 11 0.050831457410500325 0.0 0.0 0.0 0.0 12 0.050831457410500325 0.0 0.0 0.0 0.0 13 0.050831457410500325 0.0 0.0 0.0 0.0 14 0.06535473095635756 0.0 0.0 0.0 0.0 15 0.06535473095635756 0.0 0.0 0.0 0.0 16 0.06535473095635756 0.0 0.0 0.0 0.0 17 0.07261636772928617 0.0 0.0 0.0 0.0 18 0.07261636772928617 0.0 0.0 0.0 0.0 19 0.07261636772928617 0.0 0.0 0.0 0.0 20 0.07261636772928617 0.0 0.0 0.0 0.0 21 0.08713964127514341 0.0 0.0 0.0 0.0 22 0.11618618836685789 0.0 0.0 0.0 0.0 23 0.12344782513978651 0.0 0.0 0.0 0.0 24 0.15249437223150097 0.0 0.0 0.0 0.0 25 0.17427928255028682 0.0 0.0 0.0 0.0 26 0.18154091932321545 0.0 0.0 0.0 0.0 27 0.18880255609614408 0.0 0.0 0.0 0.0 28 0.2396340135066444 0.0 0.0 0.0 0.0 29 0.2541572870525016 0.0 0.0 0.0 0.0 30 0.2686805605983589 0.0 0.0 0.0 0.0 31 0.2904654709171447 0.0 0.0 0.0 0.0 32 0.2977271076900733 0.0 0.0 0.0 0.0 33 0.2977271076900733 0.0 0.0 0.0 0.0 34 0.2977271076900733 0.0 0.0 0.0 0.0 35 0.33403529155471645 0.0 0.0 0.0 0.0 36 0.34855856510057365 0.0 0.0 0.0 0.0 37 0.34855856510057365 0.0 0.0 0.0 0.0 38 0.3558202018735023 0.0 0.0 0.0 0.0 39 0.3630818386464309 0.0 0.0 0.0 0.0 40 0.3703434754193595 0.0 0.0 0.0 0.0 41 0.4066516592840026 0.0 0.0 0.0 0.0 42 0.42117493282985985 0.0 0.0 0.0 0.0 43 0.4356982063757171 0.0 0.0 0.0 0.0 44 0.4429598431486457 0.0 0.0 0.0 0.0 45 0.46474475346743155 0.0 0.0 0.0 0.0 46 0.4792680270132888 0.0 0.0 0.0 0.0 47 0.4792680270132888 0.0 0.0 0.0 0.0 48 0.49379130055914605 0.0 0.0 0.0 0.0 49 0.49379130055914605 0.0 0.0 0.0 0.0 50 0.49379130055914605 0.0 0.0 0.0 0.0 51 0.5010529373320747 0.0 0.0 0.0 0.0 52 0.5155762108779319 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGATG 15 0.004589549 58.000004 8 GGAGAGT 30 3.4940786E-7 58.000004 8 GGGCATT 15 0.004589549 58.000004 7 CTCTTTT 15 0.004589549 58.000004 29 AGGGATC 15 0.004589549 58.000004 6 ACTCAGT 15 0.004589549 58.000004 34 CTCAGTA 15 0.004589549 58.000004 35 CCCTGCG 15 0.004589549 58.000004 19 GGAAGTA 15 0.004589549 58.000004 8 GCATGGT 30 3.4940786E-7 58.000004 12 GGAAGAT 15 0.004589549 58.000004 8 GGGATAT 15 0.004589549 58.000004 7 AGCATGT 15 0.004589549 58.000004 11 AGCATGG 30 3.4940786E-7 58.000004 11 GAGAGTG 15 0.004589549 58.000004 9 GGGAATT 15 0.004589549 58.000004 10 GGGGTCG 15 0.004589549 58.000004 7 GGGAAGT 30 3.4940786E-7 58.000004 7 GGAATTC 15 0.004589549 58.000004 11 GGGTGTA 15 0.004589549 58.000004 7 >>END_MODULE