FastQCFastQC Report
Fri 17 Jun 2016
SRR1526621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13098
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1921.4658726523133303No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1541.1757520232096503No Hit
CGAGTAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA1040.7940143533363873No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.465719957245381No Hit
CGAGTAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG470.3588334096808673No Hit
CGAGTAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG460.3511986562834021No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG390.2977553825011452No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.29012062910368No Hit
CGAGTAGGGATATTGATATGCCGAGAAGCACTAACATTTTTGATTGGGTCCAGCTGCCCATTGT340.2595816155138189No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG340.2595816155138189No Hit
CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.24431210871888837No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.19850358833409681No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA250.19086883493663157No Hit
CGAGTAGGGGAAGAAAACATTGATTATGACTACATAGCAACCTTATGTGAAGGTTTTAGTGGTT250.19086883493663157No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1603298213467705No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1603298213467705No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.15269506794930524No Hit
CGAGTAGGGACATGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.14506031455184No Hit
CGAGTAGGGACTGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1374255611543747No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG170.12979080775690946No Hit
CGAGTAGGGAAGCAGTGGTACAACGAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.12979080775690946No Hit
CGAGTAGGAAGCATGTATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12215605435944418No Hit
CGAGTAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12215605435944418No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11452130096197893No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG150.11452130096197893No Hit
CGAGTAGGGAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11452130096197893No Hit
CGAGTAGGGATATTCAGGGATGTTTCTTTTTATGCTACTTGTATCTGATCCTCTTCCAACTCAG140.10688654756451366No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAA140.10688654756451366No Hit
CGAGTAGGGTGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10688654756451366No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGACAGTGGTCACGCAGAGTCGACTTTTTTCTTTTTTT140.10688654756451366No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10688654756451366No Hit
CGAGTAGGGGAGAATGGGCAGACTGCTCATTGAGCCAAAAAATAAAAAAATAAAAATAAAAAGT140.10688654756451366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG150.004331924558.82745436
GTTGATG150.004331924558.82745436
TGATGCT150.004399982358.59765638
TGGTTGA201.8402073E-458.59765634
CTGAGTT150.004399982358.59765638
GATGCTG150.004399982358.59765639
CCGCTGA150.004399982358.59765635
ACCCGCT201.8402073E-458.59765633
TTGATGC150.004399982358.59765637
GCTGAGT150.004399982358.59765637
ATAAGCG201.876303E-458.3696556
ACTGACG201.876303E-458.3696557
TAAGCGG201.876303E-458.3696557
TATAAAT201.876303E-458.3696551
TCGAACT201.876303E-458.3696553
AAATAAG201.876303E-458.3696554
CTGACGG201.876303E-458.3696558
ACATCGA201.876303E-458.3696550
ATAAATA201.876303E-458.3696552
CGAACTG201.876303E-458.3696554
Produced by FastQC (version 0.11.5)