##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13098
Sequences flagged as poor quality	0
Sequence length	64
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.54641930065659	38.0	38.0	40.0	33.0	40.0
2	36.986104748816615	38.0	37.0	40.0	33.0	40.0
3	35.86547564513666	38.0	35.0	39.0	28.0	40.0
4	36.86074209803024	38.0	37.0	40.0	32.0	40.0
5	35.066346007023974	37.0	34.0	39.0	27.0	40.0
6	37.387387387387385	38.0	38.0	40.0	33.0	40.0
7	37.48419606046725	38.0	38.0	40.0	34.0	40.0
8	37.385555046571994	38.0	38.0	40.0	34.0	40.0
9	37.53534890823026	38.0	38.0	40.0	34.0	40.0
10	36.593449381584975	38.0	36.0	40.0	31.0	40.0
11	37.30309970987937	39.0	37.0	40.0	33.0	40.0
12	37.21201710184761	39.0	36.0	40.0	32.0	40.0
13	37.05558100473355	39.0	36.0	40.0	32.0	40.0
14	37.140097724843486	39.0	36.0	40.0	33.0	40.0
15	36.8970071766682	39.0	36.0	40.0	32.0	40.0
16	36.665979538860896	39.0	36.0	40.0	31.0	40.0
17	36.444342647732476	39.0	36.0	40.0	31.0	40.0
18	36.74927469842724	39.0	36.0	40.0	31.0	40.0
19	36.53389830508475	38.0	36.0	40.0	31.0	40.0
20	36.715681783478395	39.0	36.0	40.0	31.0	40.0
21	36.58787601160483	38.0	36.0	40.0	31.0	40.0
22	36.34310581768209	38.0	35.0	40.0	31.0	40.0
23	36.28683768514277	38.0	35.0	40.0	30.0	40.0
24	35.92006413192854	38.0	35.0	40.0	29.0	40.0
25	36.05924568636433	38.0	35.0	40.0	30.0	40.0
26	36.175141242937855	39.0	35.0	40.0	30.0	40.0
27	35.846388761643	39.0	35.0	40.0	29.0	40.0
28	35.81607879065506	39.0	35.0	40.0	29.0	40.0
29	35.71499465567262	39.0	35.0	40.0	29.0	40.0
30	35.61429225836005	39.0	35.0	40.0	28.0	40.0
31	35.60322186593373	39.0	35.0	40.0	29.0	40.0
32	35.63360818445564	39.0	35.0	40.0	29.0	40.0
33	35.71675064895404	39.0	35.0	40.0	29.0	40.0
34	35.29981676591846	39.0	35.0	40.0	27.0	40.0
35	35.295006871278055	39.0	35.0	40.0	27.0	40.0
36	35.14597648495953	39.0	35.0	40.0	27.0	40.0
37	34.97930981829287	39.0	35.0	40.0	27.0	40.0
38	34.93663154680104	39.0	35.0	40.0	26.0	40.0
39	34.53901358986105	39.0	35.0	40.0	24.0	40.0
40	34.76836158192091	39.0	35.0	40.0	26.0	40.0
41	34.589173919682395	39.0	35.0	40.0	25.0	40.0
42	34.415712322491984	39.0	35.0	40.0	24.0	40.0
43	34.37792029317453	38.0	34.0	40.0	24.0	40.0
44	34.06100167964575	38.0	34.0	40.0	23.0	40.0
45	34.098641013895254	38.0	34.0	40.0	23.0	40.0
46	33.837914185371815	38.0	34.0	40.0	19.0	40.0
47	33.709573980760425	38.0	34.0	40.0	17.0	40.0
48	33.5442815697053	38.0	34.0	40.0	16.0	40.0
49	33.197663765460376	38.0	33.0	39.0	13.0	40.0
50	32.76400977248435	38.0	33.0	39.0	4.0	40.0
51	32.286761337608795	38.0	32.0	39.0	4.0	40.0
52	32.15910826080317	37.0	32.0	39.0	4.0	40.0
53	31.744388456252864	37.0	32.0	39.0	4.0	40.0
54	31.326309360207667	37.0	31.0	39.0	4.0	40.0
55	30.84722858451672	36.0	30.0	39.0	4.0	40.0
56	29.70277905023668	36.0	28.0	39.0	4.0	40.0
57	29.180943655519926	35.0	26.0	39.0	4.0	40.0
58	28.85364177737059	35.0	24.0	39.0	4.0	40.0
59	28.29767903496717	35.0	20.0	39.0	4.0	40.0
60	27.839441136051306	35.0	15.0	39.0	4.0	40.0
61	27.159413650939076	35.0	4.0	39.0	4.0	40.0
62	26.987326309360206	35.0	4.0	39.0	4.0	40.0
63	26.56924721331501	34.0	4.0	39.0	4.0	40.0
64	26.449152542372882	35.0	4.0	39.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	1.0508842060123982
2	2	1.4513356064638003
2	3	2.32008238674905
2	4	1.935385246282678
2	5	1.7444729060113673
2	6	1.3616880982265869
2	7	1.4325962261859573
2	8	1.837869205817924
2	9	1.6376955428237352
2	10	2.0331805658728825
2	11	-0.08869530279784499
2	12	-0.7966554101169407
2	13	0.7254147561839872
2	14	-0.592932921779088
2	15	0.6917437737950536
2	16	0.6142082276697565
2	17	1.0171047138995846
2	18	0.979121685531922
2	19	1.3440229049203367
2	20	1.6646509473432545
2	21	1.0114462223436576
2	22	0.4092504124555205
2	23	1.9748377927865022
2	24	0.48605274118095565
2	25	2.1331427831427803
2	26	0.9330153227589264
2	27	2.2027618867362406
2	28	2.18118621644264
2	29	1.2089338249594732
2	30	1.7721884952654108
2	31	1.5791830202086672
2	32	1.1752676810369138
2	33	1.1422598531572845
2	34	1.4858349627580338
2	35	2.101117258809566
2	36	0.6858856243471507
2	37	0.38258638322740524
2	38	-0.40536268100370165
2	39	-3.4300765711022265
2	40	1.3024279139663761
2	41	-0.3055524722191336
2	42	-0.07067962901296454
2	43	-2.292041078579537
2	44	-1.4446741292895169
2	45	-1.6839977211772066
2	46	-4.309718486641554
2	47	-5.534993140762371
2	48	-4.52010795472334
2	49	-5.347458832715251
2	50	-4.757291402804217
2	51	-3.1334751905264717
2	52	-3.1783785540195737
2	53	-5.1937476578502135
2	54	-5.2403232569899245
2	55	-1.6365275749891097
2	56	-4.347907268420098
2	57	-4.342185378723844
2	58	-1.9781750775340434
2	59	-0.6571301205916598
2	60	-0.7121114640345354
2	61	-1.7189256612333565
2	62	-0.5034009200675769
2	63	-1.6023852453339558
2	64	-0.43121444082982663
3	1	-0.827320922192726
3	2	0.37441252954072013
3	3	1.0252105918772543
3	4	-0.7697429588455265
3	5	-0.3580911965527349
3	6	0.412970149508638
3	7	-0.009711466121736123
3	8	0.3058179237666465
3	9	0.0992340043621951
3	10	-0.68476815207584
3	11	-2.877156841259385
3	12	-3.2902451537066852
3	13	-3.261764730995502
3	14	-2.5608816397278105
3	15	-1.8210767390254574
3	16	-2.6486122851507545
3	17	-1.8739209271260577
3	18	-1.9247244683142313
3	19	-2.0341822232847875
3	20	-1.2007336680413587
3	21	-2.02060505970762
3	22	-2.257416254211144
3	23	-3.1982391302904176
3	24	-3.4626652075369933
3	25	-2.7386520886520955
3	26	-2.861856472112869
3	27	-1.7523663183919638
3	28	-2.7611214758650533
3	29	-2.0346559186302713
3	30	-2.349606376529465
3	31	-1.222099031073384
3	32	-1.1452451394759038
3	33	-1.8705606596632265
3	34	-1.2962163192932437
3	35	-0.8155494078570982
3	36	-5.775652837191309
3	37	-7.040490539849518
3	38	-6.219465245106267
3	39	-26.930076571102227
3	40	-6.556546445007982
3	41	-6.888885805552469
3	42	-6.339910398243735
3	43	-6.702297488835949
3	44	-6.585699770315159
3	45	-6.119895157074641
3	46	-1.841769768692835
3	47	-1.7914033971726226
3	48	-2.7765182111335953
3	49	-3.052587037843459
3	50	-3.3983170438298558
3	51	-4.0245008315521105
3	52	-5.75530163094265
3	53	-4.6488758629784215
3	54	-4.669810436477103
3	55	-4.61729680575834
3	56	-1.7132918838047146
3	57	-1.278082814621282
3	58	-1.3756109749699412
3	59	-0.9520019154634518
3	60	-0.28903454095761205
3	61	1.1528692105615157
3	62	0.8299324132657553
3	63	2.455307062358351
3	64	2.1713496617342756
4	1	-1.6157824606542661
4	2	-1.7986643935361997
4	3	-3.013250946584286
4	4	-2.3979480870506507
4	5	-4.505527093988636
4	6	-1.6383119017734131
4	7	-1.8174037738140427
4	8	-1.662130794182076
4	9	-1.8623044571762648
4	10	-2.133486100793789
4	11	-0.588695302797845
4	12	-0.46332207678360504
4	13	-0.6079185771493485
4	14	-0.6762662551124237
4	15	-0.641589559538275
4	16	-0.21912510566357213
4	17	1.0171047138995846
4	18	-1.3542116478014137
4	19	0.2606895715870081
4	20	-0.16868238599008123
4	21	0.8447795556769933
4	22	0.7425837457888562
4	23	-0.10849554054683352
4	24	1.2360527411809556
4	25	0.13314278314278027
4	26	-1.483651343907745
4	27	-1.7972381132637594
4	28	-1.4854804502240242
4	29	-1.457732841707191
4	30	-0.8944781714012606
4	31	-1.1708169797913328
4	32	-5.824732318963083
4	33	-4.27440681350938
4	34	-4.014165037241963
4	35	-7.815549407857098
4	36	-3.3141143756528493
4	37	-4.034080283439263
4	38	-4.488696014337037
4	39	-2.7634099044355622
4	40	-3.030905419366956
4	41	-1.972219138885805
4	42	-1.6540129623463002
4	43	-2.7920410785795404
4	44	-1.0280074626228526
4	45	-1.4339977211772066
4	46	-3.7263851533082217
4	47	-6.451659807429035
4	48	-6.853441288056672
4	49	-5.847458832715251
4	50	-5.173958069470881
4	51	-3.966808523859804
4	52	-3.261711887352906
4	53	-5.027080991183549
4	54	-3.906989923656589
4	55	-3.8865275749891097
4	56	-1.0145739350867657
4	57	-1.0088520453905119
4	58	-3.3948417442007113
4	59	-1.990463453924992
4	60	-2.0454447973678676
4	61	-1.1355923279000244
4	62	-1.4200675867342447
4	63	-0.8523852453339558
4	64	-0.9312144408298266
5	1	-1.5196286145004194
5	2	-1.5486643935361997
5	3	-2.8209432542765924
5	4	-1.6928198819224463
5	5	-3.435014273475815
5	6	-2.510106773568289
5	7	-1.1635576199678894
5	8	-0.8480282300795068
5	9	-1.0546121494839582
5	10	-0.7616912289989131
5	11	-3.415618379720925
5	12	-3.597937461398992
5	13	-2.954072423303195
5	14	-3.94549702434319
5	15	-2.744153662102377
5	16	-22.033227669766138
5	17	-3.1816132348183643
5	18	-3.6170321606219247
5	19	-5.188028377130944
5	20	-3.6622721295798257
5	21	-4.174451213553773
5	22	-5.180493177288067
5	23	-4.044392976444264
5	24	-4.462665207536997
5	25	-6.200190550190555
5	26	-0.3233949336513291
5	27	0.32455675853111643
5	28	0.6234939087503335
5	29	-0.03465591863027129
5	30	0.2657782388551553
5	31	-1.6836374926118438
5	32	-1.0683220625528307
5	33	-1.178252967355533
5	34	-1.7577547808317107
5	35	-7.046318638626328
5	36	-1.8525759141143894
5	37	-1.4251059244649014
5	38	-1.6040806297216506
5	39	-0.3146919557176133
5	40	-0.6334695219310547
5	41	-6.427347344014009
5	42	-23.95529501362835
5	43	-6.009989796528256
5	44	-5.431853616469006
5	45	-6.735279772459254
5	46	-3.4571543840774517
5	47	-4.022172627941856
5	48	-3.238056672672055
5	49	-3.1295101147665356
5	50	-2.167547813060626
5	51	-2.870654677705957
5	52	-5.601455477096497
5	53	-6.418106632209188
5	54	-7.20827197493864
5	55	-6.694219882681416
5	56	-6.790214960727791
5	57	-6.508852045390512
5	58	-5.5294571288160945
5	59	-5.105848069309609
5	60	-4.7505730024960755
5	61	-3.000976943284641
5	62	-5.246990663657321
5	63	-3.8523852453339558
5	64	-2.5978811074964945
6	1	0.6342175393457339
6	2	0.034668939797136034
6	3	1.1534157200823785
6	4	-1.1479480870506507
6	5	0.3278062393446959
6	6	1.6950214315599155
6	7	1.3492628928526216
6	8	1.587869205817924
6	9	1.3043622094903995
6	10	1.6998472325395468
6	11	0.911304697202155
6	12	1.8700112565497307
6	13	0.8087480895173158
6	14	0.49040041155424063
6	15	0.6917437737950536
6	16	-0.8024584389969078
6	17	1.4337713805662489
6	18	-0.9375449811347423
6	19	-0.4893104284129919
6	20	-0.3353490526567455
6	21	-0.23855377765634245
6	22	-0.09074958754447948
6	23	0.22483779278650218
6	24	0.90271940784762
6	25	1.0498094498094446
6	26	1.9330153227589264
6	27	2.0360952200695763
6	28	1.18118621644264
6	29	2.208933824959473
6	30	2.3555218285987394
6	31	2.4958496868753386
6	32	0.5919343477035852
6	33	0.9755931864906202
6	34	2.0691682960913695
6	35	2.101117258809566
6	36	-1.7307810423195136
6	37	-2.4507469501059305
6	38	-2.822029347670373
6	39	-21.09674323776889
6	40	-0.9475720860336239
6	41	-3.6388858055524693
6	42	-3.0706796290129645
6	43	-6.875374411912873
6	44	-3.0280074626228526
6	45	-3.2673310545105387
6	46	1.0236148466917783
6	47	1.2983401925709614
6	48	-0.27010795472333626
6	49	-1.1807921660485832
6	50	-0.5906247361375492
6	51	-3.550141857193136
6	52	-4.678378554019574
6	53	-6.6937476578502135
6	54	-6.2403232569899245
6	55	-5.88652757498911
6	56	-7.347907268420098
6	57	-7.258852045390512
6	58	-8.06150841086738
6	59	-8.573796787258324
6	60	-9.128778130701203
6	61	-8.552258994566692
6	62	-8.003400920067577
6	63	-8.852385245333956
6	64	-8.347881107496494
7	1	1.4675508726790696
7	2	2.2013356064638003
7	3	2.9034157200823785
7	4	2.2687185796160136
7	5	1.9111395726780316
7	6	1.8616880982265869
7	7	1.4325962261859573
7	8	1.7545358724845954
7	9	1.5543622094903995
7	10	2.366513899206211
7	11	1.161304697202155
7	12	1.8700112565497307
7	13	1.6420814228506515
7	14	2.0737337448875763
7	15	1.608410440461725
7	16	2.030874894336428
7	17	1.9337713805662489
7	18	1.479121685531922
7	19	2.1773562382536724
7	20	-0.3353490526567455
7	21	0.17811288901032896
7	22	0.4925837457888562
7	23	0.14150445945316648
7	24	-0.01394725881904435
7	25	0.13314278314278027
7	26	0.0996819894255907
7	27	0.36942855340291203
7	28	-0.4854804502240242
7	29	0.5422671582928089
7	30	0.9388551619320751
7	31	-3.087483646457997
7	32	-3.908065652296415
7	33	-3.8577401468427155
7	34	-21.347498370575295
7	35	-3.2322160745237625
7	36	-4.730781042319517
7	37	-4.617413616772595
7	38	-4.405362681003705
7	39	-3.7634099044355587
7	40	-4.364238752700288
7	41	-0.8055524722191336
7	42	0.7626537043203712
7	43	-0.04204107857953687
7	44	0.3886592040438117
7	45	-1.2673310545105352
7	46	1.356948180025114
7	47	1.7983401925709614
7	48	1.7298920452766637
7	49	0.9858745006180811
7	50	1.9093752638624508
7	51	-0.8834751905264717
7	52	-0.6783785540195737
7	53	0.3895856754831186
7	54	0.25967674301007904
7	55	-1.5531942416557776
7	56	-3.2645739350867657
7	57	-3.092185378723844
7	58	-2.6448417442007113
7	59	-3.573796787258324
7	60	-1.9621114640345354
7	61	-4.2189256612333565
7	62	-3.253400920067577
7	63	-3.435718578667288
7	64	-2.7645477741631623
8	1	-1.2824491273209304
8	2	0.28466893979713603
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118	35	1.722912130604442
118	36	2.378193316654844
118	37	3.1133556139966387
118	38	3.0113039856629626
118	39	2.2237695827439268
118	40	-0.5565464450079816
118	41	1.9572680406013774
118	42	-1.262987321320658
118	43	2.143856357317894
118	44	-0.970315154930546
118	45	0.8801048429253626
118	46	2.235153308230238
118	47	-0.5606341664033963
118	48	0.45425101963563463
118	49	0.2551052698488476
118	50	0.14014449463168432
118	51	1.2831914761401961
118	52	0.0908522152111928
118	53	1.3511241370215785
118	54	1.1763434096767433
118	55	0.07501088654935018
118	56	-1.9440611145739446
118	57	-3.2011597376982053
118	58	-2.990995590354558
118	59	-3.3366173000788386
118	60	-5.135188387111459
118	61	-4.231746174053871
118	62	-4.016221432888091
118	63	-5.160077553026262
118	64	-3.520958030573418
119	1	-0.3657824606542661
119	2	-0.8819977268695354
119	3	-1.1799176132509501
119	4	-0.39794808705065066
119	5	-2.422193760655304
119	6	-0.22164523510674883
119	7	-0.5674037738140427
119	8	0.2545358724845954
119	9	-0.2789711238429291
119	10	-0.6334861007937889
119	11	0.4946380305354907
119	12	0.45334458988305926
119	13	-0.3579185771493485
119	14	-0.5095995884457594
119	15	0.858410440461725
119	16	0.11420822766975647
119	17	1.2671047138995846
119	18	-1.020878314468078
119	19	0.5106895715870081
119	20	0.4979842806765902
119	21	-2.071887110989671
119	22	-1.0907495875444795
119	23	-0.6918288738801621
119	24	-0.7639472588190444
119	25	0.13314278314278027
119	26	0.1830153227589264
119	27	0.4527618867362406
119	28	-0.4854804502240242
119	29	0.7089338249594732
119	30	0.18885516193207508
119	31	0.9125163535420029
119	32	0.1752676810369138
119	33	1.558926519823956
119	34	1.6525016294247052
119	35	1.6844505921429018
119	36	1.352552291013815
119	37	1.3825863832274052
119	38	1.5946373189962983
119	39	2.5699234288977735
119	40	2.2190945806330475
119	41	1.3611141944475307
119	42	1.3459870376536998
119	43	1.9579589214204631
119	44	-1.1113407959561883
119	45	2.0660022788227934
119	46	1.856948180025114
119	47	-0.11832647409570285
119	48	0.31322537860999233
119	49	-0.26412549938191887
119	50	0.4093752638624508
119	51	1.2831914761401961
119	52	1.2382881126470906
119	53	2.2229190088164543
119	54	1.8430100763434076
119	55	2.2801390916775546
119	56	-5.014573935086766
119	57	-4.92551871205718
119	58	-4.644841744200711
119	59	-6.740463453924992
119	60	-5.878778130701203
119	61	-5.9689256612333565
119	62	-4.503400920067577
119	63	-4.435718578667288
119	64	-5.431214440829827
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	43.0
5	1.0
6	3.0
7	11.0
8	12.0
9	12.0
10	19.0
11	25.0
12	26.0
13	32.0
14	43.0
15	44.0
16	42.0
17	70.0
18	54.0
19	62.0
20	65.0
21	74.0
22	90.0
23	112.0
24	114.0
25	138.0
26	182.0
27	230.0
28	306.0
29	367.0
30	416.0
31	525.0
32	584.0
33	744.0
34	913.0
35	985.0
36	1143.0
37	1571.0
38	1959.0
39	2074.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	98.53412734768668	1.0306917086578102	0.09925179416704841	0.3359291494884715
8	99.10632447296058	0.3819126183929117	0.2215093186678888	0.2902535899786129
9	97.4499923652466	1.817071308596732	0.29012062910368	0.4428156970529852
10	40.01374255611544	37.13544052527104	9.34493815849748	13.50587876011605
11	23.507405710795542	31.95144296839212	29.03496717056039	15.506184150251947
12	51.427698885326	18.842571384944264	17.590471827759963	12.139257901969765
13	24.01129943502825	19.67475950526798	29.08841044434265	27.225530615361127
14	29.25637501908688	30.187814933577645	22.17132386623912	18.384486181096353
15	35.51344743276283	23.334352078239608	20.132946210268948	21.019254278728607
16	23.360061092019855	25.719740358915615	26.865215731195114	24.054982817869416
17	27.501529987760097	27.2796817625459	17.342411260709913	27.876376988984084
18	25.76729271644526	32.16521606352114	20.247366010077876	21.82012520995572
19	22.04153305848221	26.133760879523592	31.783478393647886	20.041227668346313
20	21.128588882101404	29.131032376298105	19.341783750763593	30.398594990836898
21	28.011910215300045	25.584058634906093	24.79767903496717	21.60635211482669
22	23.652466025347383	33.46312414109025	18.666972056802567	24.217437776759812
23	34.92899679340357	31.126889601465873	18.399755687891282	15.544357917239275
24	23.379399862563947	36.26021226235016	23.646636634343743	16.713751240742155
25	24.3716097486439	29.207731683092675	26.762930705172284	19.657727863091147
26	25.5421502748931	24.824373854612094	27.504581551618816	22.128894318875993
27	22.90613994794059	25.088041647527177	27.675700505282496	24.330117899249732
28	24.535514947625966	27.999082498661977	27.999082498661977	19.46632005505008
29	24.156658762334583	26.244932303220374	31.239960223361123	18.358448711083913
30	17.387311548174793	32.54763909083952	36.15213897604653	13.91291038493916
31	18.513699678555028	29.389254553803767	37.632022041940914	14.465023725700291
32	12.111706197398622	30.54322876817138	45.11094108645754	12.234123947972456
33	13.325679295828548	31.565250669728286	40.38270187523919	14.72636815920398
34	14.059026446914528	31.00038328861633	41.90877730931392	13.031812955155232
35	12.14395099540582	33.215926493108725	42.11332312404288	12.526799387442573
36	12.000613449888812	31.508319914117017	42.113334866958056	14.377731769036117
37	12.18091222690686	29.865848984285165	45.39670371789958	12.556535070908396
38	10.432316418825692	30.239153763605703	47.792426797485824	11.536103020082784
39	12.26153846153846	30.723076923076924	45.45384615384616	11.561538461538461
40	12.562313060817548	32.27241352864483	45.64000306771992	9.5252703428177
41	10.501687634243632	32.60202516109236	46.25652040503222	10.63976679963179
42	14.585092500191909	29.37744684117602	44.783910340062945	11.253550318569125
43	12.58639225925357	31.131930579020118	46.30625095991399	9.975426201812319
44	10.461278686008136	30.854248215519224	49.36679714483076	9.317675953641876
45	12.494240516049762	31.915220396252497	45.59975426201812	9.990784825679619
46	11.381614315336764	33.79924736963367	44.75846709162123	10.06067122340834
47	11.994164171081932	34.062811948091834	44.15265299854104	9.790370882285188
48	13.869902465248446	32.71638123032025	44.10567544735427	9.30804085707703
49	9.746543778801843	34.5468509984639	45.476190476190474	10.230414746543778
50	11.200799507995079	34.524907749077485	43.519372693726936	10.754920049200493
51	9.641238380579242	33.5638011830683	45.37911961281401	11.41584082353845
52	11.087207068766809	36.61928543987706	41.651940069150974	10.641567422205148
53	11.912996695104143	34.50157558988548	43.6476827299977	9.937744985012682
54	11.729438893159108	36.01076095311299	41.383551114527286	10.876249039200616
55	11.846555965559656	36.908056580565805	40.54428044280443	10.70110701107011
56	11.788680406028915	37.796062749923095	39.10335281451861	11.311904029529375
57	11.117090797263012	37.47981855923733	39.43261320827247	11.970477435227185
58	12.162474036464344	37.61058542964844	36.848988383721824	13.377952150165399
59	12.900992384029541	38.25678898376798	37.14131856296638	11.700900069236095
60	14.913428241631395	36.837245094267026	35.313582146979606	12.93574451712197
61	12.866261631931092	38.03737599015612	36.19164808121203	12.904714296700762
62	14.527416749980773	37.591325078827964	35.068830269937706	12.812427901253557
63	15.998769325436504	39.38927774786555	33.70509960772248	10.906853318975465
64	15.64327485380117	39.258233302554636	33.12557710064635	11.972914742997846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	2.5
8	5.0
9	28.5
10	69.5
11	87.0
12	90.5
13	95.0
14	96.0
15	119.5
16	196.5
17	250.0
18	242.0
19	234.0
20	275.0
21	345.0
22	374.0
23	464.5
24	602.5
25	650.0
26	717.0
27	847.5
28	911.0
29	752.5
30	584.0
31	574.0
32	507.0
33	440.0
34	452.5
35	429.5
36	394.0
37	382.5
38	383.0
39	395.0
40	370.0
41	366.5
42	388.0
43	369.5
44	351.0
45	346.0
46	372.5
47	404.0
48	331.0
49	284.0
50	310.0
51	287.0
52	253.5
53	243.0
54	298.0
55	459.5
56	566.0
57	437.5
58	309.0
59	302.0
60	261.5
61	228.0
62	215.5
63	173.5
64	144.0
65	121.5
66	83.5
67	68.0
68	66.0
69	64.0
70	46.0
71	21.5
72	15.0
73	16.0
74	10.5
75	4.0
76	4.5
77	5.0
78	5.0
79	3.5
80	1.5
81	1.0
82	0.5
83	0.0
84	1.5
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.04580852038479157
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.07634753397465262
16	0.022904260192395786
17	0.19850358833409681
18	0.0
19	0.0
20	0.015269506794930523
21	0.0
22	0.0
23	0.0
24	0.007634753397465261
25	0.06871278057718735
26	0.015269506794930523
27	0.2748511223087494
28	0.14506031455184
29	0.19086883493663157
30	0.23667735532142314
31	0.24431210871888837
32	0.2137730951290273
33	0.25194686211635364
34	0.4046419300656589
35	0.29012062910368
36	0.4351809436555199
37	0.4046419300656589
38	0.39700717666819363
39	0.7482058329515957
40	0.45045045045045046
41	0.4733547106428463
42	0.5420674912200335
43	0.58024125820736
44	0.5267979844251031
45	0.58024125820736
46	0.5878760116048252
47	0.5726065048098946
48	0.5878760116048252
49	0.5955107650022904
50	0.6871278057718736
51	0.6184150251946862
52	0.6336845319896167
53	0.6642235455794777
54	0.671858298976943
55	0.6871278057718736
56	0.7176668193617346
57	0.6947625591693388
58	0.7558405863490609
59	0.7558405863490609
60	0.786379599938922
61	0.7253015727591998
62	0.7253015727591998
63	0.7405710795541304
64	0.7787448465414567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	13098.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.09421285692471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56850250341374	56.069628950984885
2	8.431952662721894	11.314704535043518
3	3.1520254893035955	6.344480073293633
4	1.7865270823850705	4.794625133608185
5	0.9558488848429678	3.20659642693541
6	0.5348202093764224	2.153000458085204
7	0.36413290851160673	1.7101847610322185
8	0.20482476103777877	1.0994044892349977
9	0.19344560764679108	1.168117269812185
>10	0.7624032771961766	8.237898915865017
>50	0.01137915339098771	0.465719957245381
>100	0.03413746017296313	3.435639028859368
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	1.4658726523133303	No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	154	1.1757520232096503	No Hit
CGAGTAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA	104	0.7940143533363873	No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	0.465719957245381	No Hit
CGAGTAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG	47	0.3588334096808673	No Hit
CGAGTAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG	46	0.3511986562834021	No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	39	0.2977553825011452	No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.29012062910368	No Hit
CGAGTAGGGATATTGATATGCCGAGAAGCACTAACATTTTTGATTGGGTCCAGCTGCCCATTGT	34	0.2595816155138189	No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG	34	0.2595816155138189	No Hit
CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.24431210871888837	No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.19850358833409681	No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA	25	0.19086883493663157	No Hit
CGAGTAGGGGAAGAAAACATTGATTATGACTACATAGCAACCTTATGTGAAGGTTTTAGTGGTT	25	0.19086883493663157	No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.1603298213467705	No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.1603298213467705	No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.15269506794930524	No Hit
CGAGTAGGGACATGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.14506031455184	No Hit
CGAGTAGGGACTGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.1374255611543747	No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG	17	0.12979080775690946	No Hit
CGAGTAGGGAAGCAGTGGTACAACGAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.12979080775690946	No Hit
CGAGTAGGAAGCATGTATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.12215605435944418	No Hit
CGAGTAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16	0.12215605435944418	No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.11452130096197893	No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	15	0.11452130096197893	No Hit
CGAGTAGGGAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.11452130096197893	No Hit
CGAGTAGGGATATTCAGGGATGTTTCTTTTTATGCTACTTGTATCTGATCCTCTTCCAACTCAG	14	0.10688654756451366	No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAA	14	0.10688654756451366	No Hit
CGAGTAGGGTGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.10688654756451366	No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGACAGTGGTCACGCAGAGTCGACTTTTTTCTTTTTTT	14	0.10688654756451366	No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.10688654756451366	No Hit
CGAGTAGGGGAGAATGGGCAGACTGCTCATTGAGCCAAAAAATAAAAAAATAAAAATAAAAAGT	14	0.10688654756451366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.015269506794930525	0.0	0.0	0.0	0.0
7	0.015269506794930525	0.0	0.0	0.0	0.0
8	0.015269506794930525	0.0	0.0	0.0	0.0
9	0.022904260192395786	0.0	0.0	0.0	0.0
10	0.022904260192395786	0.0	0.0	0.0	0.0
11	0.03817376698732631	0.0	0.0	0.0	0.0
12	0.04580852038479157	0.0	0.0	0.0	0.0
13	0.04580852038479157	0.0	0.0	0.0	0.0
14	0.04580852038479157	0.0	0.0	0.0	0.0
15	0.04580852038479157	0.0	0.0	0.0	0.0
16	0.04580852038479157	0.0	0.0	0.0	0.0
17	0.04580852038479157	0.0	0.0	0.0	0.0
18	0.04580852038479157	0.0	0.0	0.0	0.0
19	0.0610780271797221	0.0	0.0	0.0	0.0
20	0.0610780271797221	0.0	0.0	0.0	0.0
21	0.07634753397465262	0.0	0.0	0.0	0.0
22	0.1374255611543747	0.0	0.0	0.0	0.0
23	0.1603298213467705	0.0	0.0	0.0	0.0
24	0.1603298213467705	0.0	0.0	0.0	0.0
25	0.16796457474423576	0.0	0.0	0.0	0.0
26	0.175599328141701	0.0	0.0	0.0	0.0
27	0.1832340815391663	0.0	0.0	0.0	0.0
28	0.2214078485264926	0.0	0.0	0.0	0.0
29	0.2214078485264926	0.0	0.0	0.0	0.0
30	0.25194686211635364	0.0	0.0	0.0	0.0
31	0.26721636891128414	0.0	0.0	0.0	0.0
32	0.26721636891128414	0.0	0.0	0.0	0.0
33	0.26721636891128414	0.0	0.0	0.0	0.0
34	0.26721636891128414	0.0	0.0	0.0	0.0
35	0.2977553825011452	0.0	0.0	0.0	0.0
36	0.30539013589861047	0.0	0.0	0.0	0.0
37	0.320659642693541	0.0	0.0	0.0	0.0
38	0.32829439609100625	0.0	0.0	0.0	0.0
39	0.351198656283402	0.0	0.0	0.0	0.0
40	0.37410291647579785	0.0	0.0	0.0	0.0
41	0.3817376698732631	0.0	0.0	0.0	0.0
42	0.39700717666819363	0.0	0.0	0.0	0.0
43	0.4199114368605894	0.0	0.0	0.0	0.0
44	0.4428156970529852	0.0	0.0	0.0	0.0
45	0.45808520384791573	0.0	0.0	0.0	0.0
46	0.496258970835242	0.0	0.0	0.0	0.0
47	0.5115284776301725	0.0	0.0	0.0	0.0
48	0.5191632310276378	0.0	0.0	0.0	0.0
49	0.5267979844251031	0.0	0.0	0.0	0.0
50	0.5420674912200336	0.0	0.0	0.0	0.0
51	0.5573369980149642	0.0	0.0	0.0	0.0
52	0.5649717514124294	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTGAG	15	0.0043319245	58.827454	36
GTTGATG	15	0.0043319245	58.827454	36
TGATGCT	15	0.0043999823	58.597656	38
TGGTTGA	20	1.8402073E-4	58.597656	34
CTGAGTT	15	0.0043999823	58.597656	38
GATGCTG	15	0.0043999823	58.597656	39
CCGCTGA	15	0.0043999823	58.597656	35
ACCCGCT	20	1.8402073E-4	58.597656	33
TTGATGC	15	0.0043999823	58.597656	37
GCTGAGT	15	0.0043999823	58.597656	37
ATAAGCG	20	1.876303E-4	58.36965	56
ACTGACG	20	1.876303E-4	58.36965	57
TAAGCGG	20	1.876303E-4	58.36965	57
TATAAAT	20	1.876303E-4	58.36965	51
TCGAACT	20	1.876303E-4	58.36965	53
AAATAAG	20	1.876303E-4	58.36965	54
CTGACGG	20	1.876303E-4	58.36965	58
ACATCGA	20	1.876303E-4	58.36965	50
ATAAATA	20	1.876303E-4	58.36965	52
CGAACTG	20	1.876303E-4	58.36965	54
>>END_MODULE