FastQCFastQC Report
Fri 17 Jun 2016
SRR1526620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11159
Sequences flagged as poor quality0
Sequence length56
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2982.670490187292768No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2171.944618693431311No Hit
CGAGTAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA980.8782148938076889No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.8423693879379872No Hit
CGAGTAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA560.5018370821758221No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG400.3584550586970159No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.34053230576216503No Hit
CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.3315709292947397No Hit
CGAGTAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA330.29572542342503805No Hit
CGAGTAGGGATATTGATATGCCGAGAAGCACTAACATTTTTGATTGGGTCCAGCTG330.29572542342503805No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.2778026704901873No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC260.2329957881530603No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2150730352182095No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2150730352182095No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2150730352182095No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.20611165875078413No Hit
CGAGTAGGGAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.19715028228335874No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC200.17922752934850794No Hit
CGAGTAGGGACTGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.15234339994623175No Hit
CGAGTAGGGGAGAATGGGCAGACTGCTCATTGAGCCAAAAAATAAAAAAATAAAAA170.15234339994623175No Hit
CGAGTAGGGAGCCTCTTCCCTCCTTCACATCCGTTCCCCATCTCATACCACCGTGC170.15234339994623175No Hit
CGAGTAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15234339994623175No Hit
CGAGTACCAATAAAGACATCCGAGTGTCCTGCTGCTCCTCTGAAAAAAAAAAAAAA170.15234339994623175No Hit
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA160.14338202347880635No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.14338202347880635No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAA160.14338202347880635No Hit
CGAGTAGGGAGATGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT160.14338202347880635No Hit
CGAGTAGGGATGTTAATTATGGTCATTCCAACCCTAATCTCATGTATGATGTGAAA150.13442064701138096No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA150.13442064701138096No Hit
CGAGTAGGGGACCTCTCACCGACTTGACGCCGACGAAGACAGAGCCACCGCCGGAG150.13442064701138096No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA150.13442064701138096No Hit
CGAGTAGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAA150.13442064701138096No Hit
CGAGTAGGAAGCATGTATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT150.13442064701138096No Hit
CGAGTAGGGATGGTTCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12545927054395553No Hit
CGAGTAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12545927054395553No Hit
CGAGTAGGGATGGTACACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12545927054395553No Hit
CGAGTAGGGCTCCAGAGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12545927054395553No Hit
CGAGTAGGGAAGCAGTGGTACAACGAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTT140.12545927054395553No Hit
CGAGTAGGGATGAGATGGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGA130.11649789407653015No Hit
CGAGTAGGGACATGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11649789407653015No Hit
CGAGTAGGGGAAGAAAACATTGATTATGACTACATAGCAACCTTATGTGAAGGTTT130.11649789407653015No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAA130.11649789407653015No Hit
CGAGTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10753651760910475No Hit
CGAGTAGGGTGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10753651760910475No Hit
CGAGTAGGGGCTGAGTAAGGGCACACGGAGGATGCCTAGGCGGCGGGAGCCGAAGA120.10753651760910475No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10753651760910475No Hit
CGAGTAGGGATACCCTTCCTCTCTGTGAAGAGGTGACCCATCTGAAGAATTAACAG120.10753651760910475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG150.00786486450.54794731
TGCCGTG150.00800877650.3181839
GTAGAGT150.00815463850.090543
GTTTGAT150.00815463850.090548
CGTAGAG150.00815463850.090542
GAGTTTG150.00815463850.090546
AGAGTTT150.00815463850.090545
TTGATCA150.00815463850.090550
TAGAGTT150.00815463850.090544
TTTGATC150.00815463850.090549
CCGTAAA251.9872725E-549.86486420
GCCGTAA251.9872725E-549.86486419
GGAGATG252.0414307E-549.6412548
AGGGGCT150.00845228249.6412546
AGGGGCG402.4465407E-949.6412546
AGGGGCC252.0414307E-549.6412546
AGGGGCA150.00845228249.6412546
AGGGGAG600.049.6412546
GGATGAG150.00845228249.6412548
GGGTTAC150.00845228249.6412547
Produced by FastQC (version 0.11.5)