FastQCFastQC Report
Fri 17 Jun 2016
SRR1526619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101799
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.8320317488383973No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC7450.7318343009263352No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG5440.5343863888643308No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5100.50098723956031No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4660.4577648110492245No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4440.43615359679368165No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.37524926570987926No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3230.31729191838819637No Hit
TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3130.30746863918113143No Hit
TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2470.24263499641450306No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.19155394453776559No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.1709250582029293No Hit
TGCAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.16994273028222281No Hit
TGCAATGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1710.16797807444080984No Hit
TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1700.16699574652010335No Hit
TGCAATGGAACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCT1580.15520781147162546No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1580.15520781147162546No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.152260827709506No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.1335965972160827No Hit
TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1350.13261426929537617No Hit
TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1320.1296672855332567No Hit
TGCAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.1296672855332567No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.11984400632619181No Hit
TGCAATGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1200.11787935048477884No Hit
TGCAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1160.11395003880195287No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1150.11296771088124638No Hit
TGCAATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC1050.10314443167418147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCACT351.591252E-858.1377150
AACCGTG150.004631242758.13770749
TTGGCCT150.004631242758.13770749
CCCTCAT150.004631242758.13770753
TAAAACC150.004631242758.13770746
ACCCTCA150.004631242758.13770752
AAAGTTT150.004631242758.13770748
TCGGAAC150.004631242758.13770751
CCTCATG150.004631242758.13770754
AAACCGT150.004631242758.13770748
AACTGAG150.004631242758.13770755
TCTCCAA150.004631242758.13770745
AACCTGC150.004631242758.13770750
ACCAATT150.004631242758.13770743
CTATCAT150.004640305458.10902439
CAAATTG351.603803E-858.0803736
ACACTAT150.004649381658.0803736
GGTTATT150.004649381658.0803736
TTATTCA150.004649381658.0803738
GTTATTC150.004649381658.0803737
Produced by FastQC (version 0.11.5)