FastQCFastQC Report
Fri 17 Jun 2016
SRR1526618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98617
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.7463216281168561No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC6870.6966344545058154No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4660.47253516128050943No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3890.3944553170345883No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3560.3609925266434793No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3460.350852287131022No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.3265157123011246No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.28696877820254113No Hit
TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2600.26364622732388937No Hit
TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2080.2109169818591115No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.1632578561505623No Hit
TGCAATGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1480.1500755447843678No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.12776701785696176No Hit
TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1250.12675299390571604No Hit
TGCAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.1216828741494874No Hit
TGCAATGGAACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCT1170.11864080229575023No Hit
TGCAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.10951458673453868No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.1074865388320472No Hit
TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1040.10545849092955575No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10038837117332712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCAGT303.6013626E-758.28600337
GTTGCCG351.558874E-858.28600337
CATGCAT258.388588E-658.2561558
ATAAATC258.388588E-658.2561555
ACTGACG150.004593577258.25614557
AGGGTGG150.004593577258.25614555
CTGACGG150.004593577258.25614558
GTCAGAC150.004593577258.25614556
TCGTCTA150.004593577258.25614558
TCATCGT150.004593577258.25614555
CATCGTC150.004593577258.25614556
TAAATCA303.6141682E-758.25614556
ACTCAAG303.6141682E-758.25614558
AACATCG201.9630782E-458.22632249
ATTCGTA150.004602907758.2263250
CGTAGAT150.004602907758.2263247
CACTACT258.414123E-658.2263249
AAATTCG150.004602907758.2263248
TCCAACC150.004602907758.2263247
ATCGATA150.004602907758.2263247
Produced by FastQC (version 0.11.5)