##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526618_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98617 Sequences flagged as poor quality 0 Sequence length 64 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.160023119746086 39.0 38.0 40.0 35.0 40.0 2 37.67252096494519 39.0 38.0 40.0 33.0 40.0 3 37.87878357686809 39.0 38.0 40.0 34.0 40.0 4 37.53776732206415 39.0 38.0 40.0 33.0 40.0 5 38.17937069673586 39.0 38.0 40.0 35.0 40.0 6 37.925469239583435 39.0 38.0 40.0 35.0 40.0 7 38.044252005232366 39.0 38.0 40.0 35.0 40.0 8 37.997292556050176 39.0 38.0 40.0 35.0 40.0 9 38.15395925651764 39.0 38.0 40.0 35.0 40.0 10 37.46660312116572 39.0 38.0 40.0 33.0 40.0 11 37.63287262845149 39.0 38.0 40.0 33.0 40.0 12 37.434803330054656 39.0 38.0 40.0 33.0 40.0 13 37.38866524027298 39.0 37.0 40.0 33.0 40.0 14 37.28998042933774 39.0 37.0 40.0 33.0 40.0 15 37.122017502053396 39.0 37.0 40.0 33.0 40.0 16 36.86815660585903 39.0 36.0 40.0 32.0 40.0 17 36.62788363061136 39.0 36.0 40.0 31.0 40.0 18 36.93381465670219 39.0 36.0 40.0 32.0 40.0 19 36.764837705466604 39.0 36.0 40.0 31.0 40.0 20 36.80327935345833 39.0 36.0 40.0 31.0 40.0 21 36.848231035217054 39.0 36.0 40.0 32.0 40.0 22 36.658902623279964 38.0 36.0 40.0 31.0 40.0 23 36.50613991502479 38.0 36.0 40.0 31.0 40.0 24 36.3721569303467 38.0 35.0 40.0 31.0 40.0 25 36.36310169646207 38.0 36.0 40.0 31.0 40.0 26 36.532352434164494 39.0 36.0 40.0 31.0 40.0 27 36.01399353052719 39.0 36.0 40.0 29.0 40.0 28 35.970187695833374 39.0 36.0 40.0 29.0 40.0 29 35.89876998894714 39.0 36.0 40.0 29.0 40.0 30 35.86694991735705 39.0 35.0 40.0 29.0 40.0 31 35.613474350264156 39.0 35.0 40.0 28.0 40.0 32 35.708812882160274 39.0 35.0 40.0 29.0 40.0 33 35.687437257268016 39.0 35.0 40.0 29.0 40.0 34 35.07587941227172 39.0 35.0 40.0 26.0 40.0 35 35.228145248790774 39.0 35.0 40.0 27.0 40.0 36 34.73718527231613 38.0 35.0 40.0 26.0 40.0 37 34.46411876248517 38.0 34.0 40.0 24.0 40.0 38 34.47290020990296 38.0 34.0 40.0 25.0 40.0 39 34.02766257338998 38.0 33.0 40.0 21.0 40.0 40 34.159313302980216 38.0 33.0 39.0 23.0 40.0 41 33.89299005242504 38.0 34.0 39.0 21.0 40.0 42 33.589634647170364 38.0 33.0 39.0 17.0 40.0 43 33.598882545605726 38.0 33.0 39.0 19.0 40.0 44 33.414330186479006 38.0 33.0 39.0 18.0 40.0 45 33.27754849569547 38.0 33.0 39.0 17.0 40.0 46 33.10108804769968 38.0 33.0 39.0 10.0 40.0 47 32.86369490047355 38.0 33.0 39.0 9.0 40.0 48 32.750519687275016 38.0 32.0 39.0 7.0 40.0 49 32.46952351014531 38.0 32.0 39.0 4.0 40.0 50 32.06997779287547 37.0 31.0 39.0 4.0 40.0 51 31.626768204264984 37.0 31.0 39.0 4.0 40.0 52 31.507082957299453 37.0 31.0 39.0 4.0 40.0 53 31.18983542391271 36.0 30.0 39.0 4.0 40.0 54 30.82297169859152 36.0 29.0 39.0 4.0 40.0 55 30.450855329202877 36.0 29.0 39.0 4.0 40.0 56 29.77418700629709 36.0 28.0 39.0 4.0 40.0 57 29.44075565064847 35.0 27.0 39.0 4.0 40.0 58 29.19957005384467 35.0 26.0 39.0 4.0 40.0 59 28.819564578115337 35.0 24.0 39.0 4.0 40.0 60 28.450652524412625 35.0 23.0 39.0 4.0 40.0 61 28.15021750813754 35.0 19.0 39.0 4.0 40.0 62 27.869697922264923 35.0 16.0 39.0 4.0 40.0 63 27.507620389993612 35.0 4.0 39.0 4.0 40.0 64 27.412728028636035 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.4429639113557542 2 2 0.5004290142722994 2 3 0.3875522026666687 2 4 0.9345172140110236 2 5 0.3685553513553117 2 6 0.5943043497230747 2 7 -0.06621519848776813 2 8 0.22825551610678474 2 9 -0.23336655737040246 2 10 0.6449690395496646 2 11 -0.10098423114525445 2 12 0.1579120208468936 2 13 0.2199914222497057 2 14 -0.3128158517061479 2 15 0.5014046617302412 2 16 0.9625889777428895 2 17 1.0354151566600436 2 18 1.1893310620565956 2 19 0.36912660091651617 2 20 0.9531145520075199 2 21 0.7397017195890285 2 22 0.47217085202814246 2 23 0.4346184701297915 2 24 -0.2686705395155258 2 25 -0.016144080098762004 2 26 0.40899399773560674 2 27 0.9006958177897744 2 28 0.6212656219825234 2 29 0.48012166669186485 2 30 0.44683396067257775 2 31 0.8357786580527033 2 32 0.8563351588042565 2 33 0.35249366970259643 2 34 0.6352619392469308 2 35 0.965158867605723 2 36 0.35532598550770444 2 37 0.22494144512864267 2 38 0.3929053843477064 2 39 -2.593302179834218 2 40 -0.48020981131569584 2 41 -0.4312590040387789 2 42 -0.1920055663988336 2 43 -0.5551616447145449 2 44 -0.7421507823681353 2 45 -0.5184806391153813 2 46 -0.36362070348830855 2 47 -0.03605933166998909 2 48 0.24235639625761962 2 49 -0.006206657521417469 2 50 -0.061524535920241874 2 51 0.18550239728206108 2 52 -0.2053270668703142 2 53 -0.22980696167188341 2 54 -0.15306397470099853 2 55 -0.44296498911713655 2 56 0.2222288657646132 2 57 -0.041039096987098844 2 58 -0.24538484357089985 2 59 -0.5057250678539873 2 60 -0.12758181709898508 2 61 0.20244798064874914 2 62 0.2873735167799545 2 63 0.43957899577084447 2 64 -0.24363233512862337 3 1 0.005388881343748153 3 2 -0.06559739629192762 3 3 -0.35834615667707226 3 4 -0.5478757431718506 3 5 -0.26229698958106695 3 6 -0.21101777912846842 3 7 -0.40294989236532075 3 8 0.1506244636858156 3 9 -0.3810256209958496 3 10 -0.5270997879813422 3 11 -0.808867384406561 3 12 -0.8354853380966887 3 13 -0.9126616389747824 3 14 -0.9598746752355609 3 15 -1.2316885755646751 3 16 -0.1674630430654318 3 17 -1.658062234296338 3 18 -1.0687721792399287 3 19 -0.6826941273747948 3 20 -1.1301187413098077 3 21 -1.5928312936162499 3 22 -1.3953761667793785 3 23 -1.1818281085016622 3 24 -1.3142887868144442 3 25 -0.718024832399685 3 26 -1.3427066825365017 3 27 -0.6075074635227509 3 28 -1.2838964428434068 3 29 -0.9064329551568733 3 30 -1.01895235385323 3 31 -1.477946832143374 3 32 -1.0700353894150325 3 33 -0.8557896436227352 3 34 -1.2701002056110156 3 35 -1.2393229251113667 3 36 -6.015022153747996 3 37 -5.542765637704488 3 38 -5.831784491602672 3 39 -23.18633939472017 3 40 -7.674887682464156 3 41 -6.561511104879113 3 42 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-2.4020175679469276 119 62 -1.963586273076583 119 63 -2.341184794893934 119 64 -2.0100497932009596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 72.0 5 10.0 6 28.0 7 44.0 8 146.0 9 92.0 10 143.0 11 164.0 12 196.0 13 241.0 14 300.0 15 364.0 16 411.0 17 496.0 18 553.0 19 591.0 20 661.0 21 866.0 22 1098.0 23 1050.0 24 1126.0 25 1301.0 26 1206.0 27 1534.0 28 1768.0 29 2200.0 30 2700.0 31 3205.0 32 3770.0 33 4756.0 34 5892.0 35 7093.0 36 8897.0 37 12480.0 38 17476.0 39 15617.0 40 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.32363916605824 0.5364241096779427 0.06692625943051837 0.07301046483329277 8 99.28375046921445 0.3774006026235429 0.22217938702837606 0.11666954113362213 9 97.48015048115435 1.392254885060385 0.43096017927943453 0.6966344545058154 10 42.055629353965344 37.60812030380157 7.278663922041839 13.057586420191244 11 26.08171004999138 32.14455925448959 19.57471835484754 22.199012340671487 12 38.40412910552947 18.47146029589219 25.273532960848534 17.850877637729802 13 24.400458338825963 16.783110417068052 32.03808673960879 26.7783445044972 14 26.885831043329244 23.140026567427523 27.379660707585913 22.59448168165732 15 30.022231922604487 19.516379546631203 25.66213568578883 24.799252844975484 16 24.951571516952505 25.90797066907372 24.897818436292457 24.242639377681314 17 27.59702674709072 27.296451999431344 20.35683096732265 24.749690286155285 18 27.1220986239695 24.264579129359035 25.701451068274235 22.911871178397234 19 27.53379234817526 23.754525081882434 24.487664398633097 24.224018171309204 20 25.967360765977297 27.342711957238343 21.62222470155082 25.067702575233536 21 28.243609114047274 20.962917144102946 26.401127594633785 24.392346147216 22 26.820933510449517 23.50406116592474 23.30936856728556 26.365636756340184 23 27.121084600018253 28.245637161949766 22.935193729275884 21.698084508756097 24 22.47472442781378 31.743279283664428 22.707959396834088 23.074036891687708 25 25.982142857142858 23.318790584415584 25.38961038961039 25.30945616883117 26 24.519747651986936 22.55512505831998 27.459074588717364 25.466052700975716 27 21.738246712331552 21.62645582227281 30.291266082643958 26.34403138275168 28 20.764644340839606 26.825527419731642 29.332358228966694 23.077470010462058 29 22.989988018114985 23.918076399748177 31.297089823521052 21.79484575861579 30 20.72179197918572 24.970018497062828 33.70429091204748 20.603898611703965 31 20.957463027900594 24.2486151344209 34.95654825430706 19.83737358337145 32 21.47558968732858 22.319741573718535 38.36980150748664 17.834867231466244 33 21.904026340928027 22.831853011117662 35.65476311457084 19.609357533383466 34 17.850598582946496 25.055989901457774 40.053139506474466 17.040272009121264 35 17.515324638358866 28.64258775452115 38.45950534202153 15.382582265098454 36 19.372398986558675 25.949592486696037 37.0720092797037 17.605999247041584 37 17.29682967787899 25.54679151188235 39.79339473818126 17.3629840720574 38 20.244418666178237 24.531412174125407 40.14083073854733 15.083338421149032 39 18.685145845248798 23.006373069695236 38.247610098864286 20.06087098619168 40 18.079671056649094 24.857003277220265 40.84108535021475 16.222240315915894 41 15.647436223840316 25.58887927083864 40.78720912470085 17.976475380620194 42 16.753990744327332 23.23907769464435 40.8187396791095 19.18819188191882 43 17.4804787049685 23.035128137168954 42.670594711411034 16.813798446451507 44 17.492206442674053 23.46421076224047 42.043440167892584 17.000142627192893 45 16.596161099275236 25.2117715415745 40.399180436488926 17.79288692266134 46 17.639861744104245 24.307955831523568 40.02385831829444 18.028324106077754 47 16.761650371608876 26.04728455350862 41.815938911374595 15.375126163507908 48 17.160854984863466 26.696362135219708 40.0328213073481 16.109961572568725 49 16.606682232032707 28.063539319542013 39.30832679112162 16.021451657303658 50 15.613898983299986 28.24098648483116 39.36139806459516 16.783716467273692 51 15.583276384369803 29.42646443834722 39.86291449495619 15.127344682326783 52 17.580747179204668 28.06614841566179 39.337087592581256 15.016016812552285 53 18.335901386748844 28.113552179103866 37.851406647006606 15.699139787140684 54 17.952799289846645 28.1663554644056 37.06265878967829 16.818186456069466 55 18.071317766165905 28.671007511430435 36.35899738732854 16.898677335075114 56 17.329345351721603 27.96418983064689 36.47676116005349 18.229703657578018 57 16.59504975759122 29.5412094922174 36.7175299821383 17.146210768053074 58 16.380868730403524 30.568974497768426 35.48660545586388 17.563551315964173 59 17.562226148770797 31.863260782971942 35.3430226026208 15.231490465636458 60 18.40602364095177 30.4665869780652 34.56646335856806 16.560926022414975 61 19.307451228600303 28.659513868329984 34.1537613442633 17.879273558806418 62 16.49427693313048 29.731357913760885 33.816637225971796 19.957727927136833 63 17.457935146614393 31.434288981458792 32.625786163522015 18.481989708404804 64 19.189462605123595 29.534748265396836 32.54514055650361 18.730648572975955 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.5 3 3.0 4 5.5 5 16.0 6 24.0 7 82.5 8 141.0 9 175.0 10 232.0 11 255.0 12 251.0 13 480.0 14 713.0 15 966.0 16 1485.0 17 1751.0 18 2069.0 19 2387.0 20 2658.5 21 3301.5 22 3673.0 23 3838.5 24 4112.0 25 4220.0 26 4028.0 27 3314.5 28 2793.0 29 2926.0 30 2772.5 31 2486.0 32 2405.0 33 2324.0 34 2230.0 35 2062.0 36 1988.0 37 2075.0 38 2064.5 39 1967.0 40 1853.0 41 1882.5 42 2026.0 43 2002.5 44 1979.0 45 2178.0 46 2185.5 47 1994.0 48 1976.0 49 2157.5 50 2357.0 51 2469.0 52 3248.0 53 3915.0 54 3979.0 55 3719.0 56 3395.0 57 3624.5 58 3854.0 59 3963.0 60 3996.5 61 3921.0 62 3483.0 63 2891.0 64 2737.0 65 2993.0 66 2414.5 67 1580.0 68 1401.5 69 1223.0 70 935.0 71 605.5 72 564.0 73 429.0 74 264.5 75 235.0 76 176.5 77 99.0 78 80.0 79 61.0 80 33.5 81 25.0 82 22.0 83 19.0 84 13.5 85 6.0 86 4.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0010140239512457284 8 0.04867314965979496 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.11154263463703012 16 0.01825243112242311 17 0.14094932922315626 18 0.0 19 0.0 20 0.02433657482989748 21 0.0 22 0.0 23 0.0 24 0.004056095804982914 25 0.057799365221006524 26 0.023322550878651753 27 0.22207124532281453 28 0.1683279759067909 29 0.13689323341817333 30 0.22612734112779742 31 0.235253556689009 32 0.17745419146800248 33 0.21801514951783163 34 0.3903992212296054 35 0.2494498920064492 36 0.3427400955210562 37 0.3670766703509537 38 0.34781021527728484 39 0.7148868856282385 40 0.36809069430219943 41 0.42791810742569736 42 0.5222223348915501 43 0.526278430696533 44 0.46543699362178936 45 0.5232363588427958 46 0.5455448857702019 47 0.5374326941602361 48 0.5171522151353215 49 0.5435168378677104 50 0.6621576401634607 51 0.5840777959175395 52 0.6033442509912084 53 0.6266668018698601 54 0.6185546102598943 55 0.6418771611385461 56 0.6651997120171979 57 0.6510033766997577 58 0.7138728616769928 59 0.7179289574819757 60 0.7463216281168561 61 0.6692558078221807 62 0.6895362868470953 63 0.6824381191883752 64 0.7666021071417707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 98617.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.12098319762313 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77612682182102 46.14011782958313 2 8.686158598285136 9.923238386890699 3 3.5912730113081612 6.154111360110326 4 2.0006745841543734 4.571219972215744 5 1.2408797997550194 3.5440137096038207 6 0.8308036427544336 2.8473792550980055 7 0.6319788393602102 2.5269476865043554 8 0.42427792867160175 1.9388137947818327 9 0.33551685572775203 1.7248547410689838 >10 1.3953240666773181 13.229970491903018 >50 0.053256643766309855 2.1588569922021557 >100 0.030178764800908912 3.7975196974152534 >500 0.00355044291775399 1.4429560826226715 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.7463216281168561 No Hit TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC 687 0.6966344545058154 No Hit TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG 466 0.47253516128050943 No Hit TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 389 0.3944553170345883 No Hit TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 356 0.3609925266434793 No Hit TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 346 0.350852287131022 No Hit TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 322 0.3265157123011246 No Hit TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.28696877820254113 No Hit TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 260 0.26364622732388937 No Hit TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 208 0.2109169818591115 No Hit TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.1632578561505623 No Hit TGCAATGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC 148 0.1500755447843678 No Hit TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.12776701785696176 No Hit TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG 125 0.12675299390571604 No Hit TGCAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.1216828741494874 No Hit TGCAATGGAACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCT 117 0.11864080229575023 No Hit TGCAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.10951458673453868 No Hit TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.1074865388320472 No Hit TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA 104 0.10545849092955575 No Hit TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.10038837117332712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0010140239512457284 0.0 0.0 0.0 0.0 8 0.004056095804982914 0.0 0.0 0.0 0.0 9 0.004056095804982914 0.0 0.0 0.0 0.0 10 0.020280479024914567 0.0 0.0 0.0 0.0 11 0.03143474248861758 0.0 0.0 0.0 0.0 12 0.03954693409858341 0.0 0.0 0.0 0.0 13 0.04360302990356632 0.0 0.0 0.0 0.0 14 0.05374326941602361 0.0 0.0 0.0 0.0 15 0.05779936522100652 0.0 0.0 0.0 0.0 16 0.06692558078221808 0.0 0.0 0.0 0.0 17 0.07605179634342964 0.0 0.0 0.0 0.0 18 0.08314996400214973 0.0 0.0 0.0 0.0 19 0.09024813166086983 0.0 0.0 0.0 0.0 20 0.10748653883204722 0.0 0.0 0.0 0.0 21 0.1105286106857844 0.0 0.0 0.0 0.0 22 0.1277670178569618 0.0 0.0 0.0 0.0 23 0.14399140107689343 0.0 0.0 0.0 0.0 24 0.15413164058935072 0.0 0.0 0.0 0.0 25 0.16224383219931654 0.0 0.0 0.0 0.0 26 0.1713700477605281 0.0 0.0 0.0 0.0 27 0.18049626332173965 0.0 0.0 0.0 0.0 28 0.2038188142003914 0.0 0.0 0.0 0.0 29 0.21497307766409443 0.0 0.0 0.0 0.0 30 0.23221148483527182 0.0 0.0 0.0 0.0 31 0.2382956285427462 0.0 0.0 0.0 0.0 32 0.24235172434772909 0.0 0.0 0.0 0.0 33 0.25452001176267786 0.0 0.0 0.0 0.0 34 0.25857610756766075 0.0 0.0 0.0 0.0 35 0.31941754464240446 0.0 0.0 0.0 0.0 36 0.3315858320573532 0.0 0.0 0.0 0.0 37 0.34375411947230194 0.0 0.0 0.0 0.0 38 0.3599785026922336 0.0 0.0 0.0 0.0 39 0.3711327661559366 0.0 0.0 0.0 0.0 40 0.3822870296196396 0.0 0.0 0.0 0.0 41 0.399525436790817 0.0 0.0 0.0 0.0 42 0.4056095804982914 0.0 0.0 0.0 0.0 43 0.41980591581573157 0.0 0.0 0.0 0.0 44 0.4319742032306803 0.0 0.0 0.0 0.0 45 0.4451565145968748 0.0 0.0 0.0 0.0 46 0.4522546822555949 0.0 0.0 0.0 0.0 47 0.46949308942677226 0.0 0.0 0.0 0.0 48 0.47963332893922955 0.0 0.0 0.0 0.0 49 0.49484368820791547 0.0 0.0 0.0 0.0 50 0.5049839277203727 0.0 0.0 0.0 0.0 51 0.5141101432815843 0.0 0.0 0.0 0.0 52 0.5232363588427958 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCAGT 30 3.6013626E-7 58.286003 37 GTTGCCG 35 1.558874E-8 58.286003 37 CATGCAT 25 8.388588E-6 58.25615 58 ATAAATC 25 8.388588E-6 58.25615 55 ACTGACG 15 0.0045935772 58.256145 57 AGGGTGG 15 0.0045935772 58.256145 55 CTGACGG 15 0.0045935772 58.256145 58 GTCAGAC 15 0.0045935772 58.256145 56 TCGTCTA 15 0.0045935772 58.256145 58 TCATCGT 15 0.0045935772 58.256145 55 CATCGTC 15 0.0045935772 58.256145 56 TAAATCA 30 3.6141682E-7 58.256145 56 ACTCAAG 30 3.6141682E-7 58.256145 58 AACATCG 20 1.9630782E-4 58.226322 49 ATTCGTA 15 0.0046029077 58.22632 50 CGTAGAT 15 0.0046029077 58.22632 47 CACTACT 25 8.414123E-6 58.22632 49 AAATTCG 15 0.0046029077 58.22632 48 TCCAACC 15 0.0046029077 58.22632 47 ATCGATA 15 0.0046029077 58.22632 47 >>END_MODULE