FastQCFastQC Report
Fri 17 Jun 2016
SRR1526617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88891
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9431.0608498048171358No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG5980.672734022566964No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.5759863203248923No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4860.5467370149958939No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA4190.4713638051096286No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3860.43423968680743835No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.3982405417871326No Hit
TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2320.2609938013972168No Hit
TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG2170.24411920216894847No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.20812005714864273No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1830.20587011058487362No Hit
TGCAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.19012048463848985No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1500.16874599228268328No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.1586212327457223No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.15412133961818406No Hit
TGCAATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC1370.15412133961818406No Hit
TGCAATGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC1280.14399658008122307No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.1406216602355694No Hit
TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1150.12937192741672385No Hit
TGCAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1150.12937192741672385No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1110.12487203428918563No Hit
TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC1090.12262208772541652No Hit
TGCAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.12149711444353196No Hit
TGCAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1060.11924716787976286No Hit
TGCAATGGAACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACT1050.1181221945978783No Hit
TGCAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1010.11362230147034008No Hit
TGCAATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1010.11362230147034008No Hit
TGCAATGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT920.10349754193337908No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC910.10237256865149452No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC900.10124759536960996No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.10124759536960996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGT700.050.5211437
ATATGCT700.050.5211434
TGCTGAC150.00806765450.5211436
CAAATAT354.8054062E-850.5211434
TTATTTC150.00806765450.5211436
ACAAATA354.8928996E-850.4059333
CATATGC700.050.4059333
AGTGGAA150.00814085150.40592633
CTTGCAC150.00814085150.40592633
TCAGATT150.00814085150.40592633
AGGATGC150.00814085150.40592633
TAGGTCA204.0111018E-450.3772131
AAAGGAT150.00815922750.37720531
GCATATG700.050.37720532
AAGCAAC204.0224043E-450.34852250
ACAAACG150.00817763350.3485250
GATTAAG650.050.3485250
CTAGCTT150.00817763350.3485250
TCAGTCT150.00817763350.3485250
TATCTCA204.045085E-450.2912448
Produced by FastQC (version 0.11.5)