FastQCFastQC Report
Fri 17 Jun 2016
SRR1526616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228924
Sequences flagged as poor quality0
Sequence length64
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC19670.8592371267320159No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC16700.7294997466408066No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC14580.6368925931750276No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC11690.5106498226485646No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10170.44425224091838345No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT8320.3634393947336234No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC7940.34683999930107806No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6580.2874316367003896No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC4460.1948244832346106No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4340.18958256888749106No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC4210.18390382834477817No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC4140.1808460449756251No Hit
TTCCGCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG3820.16686760671663958No Hit
TTCCGCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG3320.14502629693697472No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA3240.14153168737222832No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA3240.14153168737222832No Hit
TTCCGCGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC3190.13934755639426186No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT3190.13934755639426186No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG3180.13891073019866856No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3060.133668815851549No Hit
TTCCGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG3030.1323583372647691No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2890.12624277052646293No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC2860.12493229193968304No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2830.12362181335290315No Hit
TTCCGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2780.12143768237493666No Hit
TTCCGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC2730.11925355139697016No Hit
TTCCGCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT2720.11881672520137687No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC2700.11794307281019027No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2540.11095385368069752No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCAAAGGTC2460.10745924411595113No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT2300.10047002498645839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC201.971378E-458.2101158
CTTAAGG150.004619875558.1973254
GTAGCGT201.9735214E-458.1973256
AGCTAAC150.004619875558.1973255
TCTACTC150.004627920758.17175352
TATCTTG150.004627920758.17175352
AATCACT201.979963E-458.1589849
GAAACTA150.004635976658.14621451
CGATTGT201.9864211E-458.12069344
ATCAAAC202.0080696E-457.99343531
AGGAGAT150.00468453157.9934331
CCATAAC150.004688594557.98073626
TGTGCTA150.004704873557.93000825
TATATAC150.0047089557.9173421
TTATATA150.0047089557.9173420
AGCATTA150.004721194557.8793717
GCTAACT150.004721194557.8793717
AGGTACA258.7775825E-657.8540847
ACAGTAT150.00472937157.85408414
CATGCTA150.00472937157.85408414
Produced by FastQC (version 0.11.5)