FastQCFastQC Report
Fri 17 Jun 2016
SRR1526614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207562
Sequences flagged as poor quality0
Sequence length56
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC34241.6496275811564738No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC22291.073895992522716No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC11900.5733226698528633No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC11130.5362253206270897No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT7970.3839816536745647No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT7710.3714552760139139No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6700.3227951166398474No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC6200.2987059288309035No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6130.2953334425376514No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3700.17825998978618438No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3610.17392393598057448No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG3450.16621539588171244No Hit
TTCCGCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT3420.16477004461317582No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG3290.15850685578285043No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT2970.14308977558512637No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2970.14308977558512637No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC2950.14212620807276863No Hit
TTCCGCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC2850.13730837051097985No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC2790.13441766797390658No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTC2760.13297231670536996No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT2620.1262273441188657No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC2510.12092772280089806No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2370.11418275021439378No Hit
TTCCGCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC2340.11273739894585713No Hit
TTCCGCGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC2340.11273739894585713No Hit
TTCCGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT2320.1117738314334994No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC2260.10888312889642614No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2220.10695599387171063No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2160.10406529133463735No Hit
TTCCGCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTC2160.10406529133463735No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC2140.10310172382227961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCT150.0080128950.6325737
CTAGGTT150.0080128950.6325737
ATACGCG150.00804402150.58294336
AATACGC150.00805181750.57055335
GAATACG150.00806742850.54578834
CGTGCAA150.00813009550.44696832
TTTCGAA150.00821685150.3117248
CTAACCC150.00824856950.26272246
TATGTCA150.00825651450.25048450
ATGGAAT204.079013E-450.23825543
ATTAACC150.00828037950.21381440
ATATTAC150.00828834550.20160338
CGTTCAA150.00846505549.93444430
TACTCCC150.00846505549.93444430
TAGCATG150.00848936849.89823529
TAGTGCG150.00848936849.89823529
AAACTCG150.00850560549.87412327
GATACTT150.00850560549.87412327
CGATACT150.00851373249.86207626
CGACCAA150.00852186549.85003324
Produced by FastQC (version 0.11.5)