FastQCFastQC Report
Fri 17 Jun 2016
SRR1526613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273324
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.6175820637777875No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.2897659920094833No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.25866736912967764No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.2242759508861278No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT5840.21366583249184118No Hit
TATTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5300.19390906030937644No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.18146961115745414No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4040.14780992521695863No Hit
TATTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3980.14561472830779587No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.13976086988336187No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.11817476694326147No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3020.11049157776119184No Hit
TATTGTGGGACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATG2920.10683291624592059No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2890.10573531779133921No Hit
TATTGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2780.10171079012454083No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2740.10024732551843234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAA150.004611841458.22596758
TCGATGC150.004615205358.21524457
TCGCTAA150.004628680658.172451
TGCGTAC150.004645565558.11893544
TTATTAG150.00464894858.10825341
TACCGTA201.9946425E-458.07623338
AACGTGT150.004672677358.03359233
ATTAGCC258.669307E-657.9803829
ACGCGAT150.00470673257.9272624
TTCGGTA202.0201341E-457.9272625
ATCGCCG303.793266E-757.90604422
TAGGCGT150.00472725357.8636516
TTGGACG202.0329766E-457.8530719
ATGCGCC202.0329766E-457.8530713
ACGAATC258.783032E-657.8530712
ATTCGTG150.0047306857.8530614
AGATGCG202.0348166E-457.84248411
ACTAATG150.004734108757.8424810
GTGGGAT17550.052.7338835
TGGGGCT11250.052.7009286
Produced by FastQC (version 0.11.5)