FastQCFastQC Report
Fri 17 Jun 2016
SRR1526612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264262
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.5267499678349516No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6640.25126578925460336No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.2100188449341941No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.1752049102784358No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4250.16082524161627476No Hit
TATTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4020.15212175795233518No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.1498512839530466No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3590.13585002762410034No Hit
TATTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3070.1161725862969326No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.11465893696407353No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2750.10406339163406013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTC150.0045968858.27319736
ATCGATA201.9837216E-458.1431
TAGCATA150.00464222558.1289330
TACACCC201.9874611E-458.1178654
GTAGCAT150.004649230758.10679629
TTAGACA150.004652736258.09573745
ATATTAG150.004659753858.0736327
TTTAGAC150.004663265358.06258444
GTCGAGG258.615374E-658.040526
TGCCACG202.0005935E-458.040525
CATGATA150.004684376557.99638724
ACTTGAT150.00470555957.93034722
TGATCGT202.0270655E-457.88640221
TGAGTTG150.004723265857.87542715
GATACGG150.004726812757.86445611
CATTCGC150.004726812757.86445612
GTCCGTA150.004737466657.83156616
ACTGACG258.821364E-657.80965810
GATCTAC150.00474813857.7987149
GTGGGGG36100.053.6054735
Produced by FastQC (version 0.11.5)