FastQCFastQC Report
Fri 17 Jun 2016
SRR1526611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236008
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18260.773702586353005No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA8340.3533778515982509No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.2508389546117081No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA5820.24660181010813195No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5210.2207552286363174No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.2122809396291651No Hit
TATTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG4660.1974509338666486No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG4630.19617979051557577No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.16228263448696653No Hit
TATTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG3320.1406731975187282No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.1406731975187282No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2920.12372461950442358No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2620.11101318599369514No Hit
TATTGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10635232703976136No Hit
TATTGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.10508118368868852No Hit
TATTGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.10381004033761568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCC150.0079737650.69749537
TCGGGTG150.00799421850.6646536
ACTCGCC150.00799421850.6646536
TCGCTAC203.9176224E-450.65371334
TAGTATG251.9605222E-550.51194833
TTGCTTA150.008103983550.49020832
TGAAATC150.008103983550.49020832
TATGCGT402.4465407E-950.49020832
CGCTCCA150.00811778350.46848731
GTACTGG203.9891124E-450.46848731
CATACTA150.00811778350.46848731
CCATACC301.0131189E-650.22003640
TCCATAC301.0146159E-650.2092939
TACCGGA150.00828473950.2092938
GCGAAAT150.0082917550.19854748
CGATTCC150.0082917550.19854747
ACAATTA150.00830578750.17707449
TTGACGA150.00830578750.17707450
TTTGACG150.00830578750.17707449
TACTTTC150.00831281250.16634846
Produced by FastQC (version 0.11.5)