FastQCFastQC Report
Fri 17 Jun 2016
SRR1526610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160132
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.3640746384233008No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.3159893088202233No Hit
GCAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4780.2985037344191042No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3540.22106761921414833No Hit
GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3450.21544725601378864No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3340.20857792321334898No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.20857792321334898No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3140.19608822721254965No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2470.15424774560987184No Hit
GCAGGAGGGAAAGCGAAGGCATCGATTACATAAGTTCCTATGTCTCAGAGTCGGAAAACCAATG2190.13676217120875278No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2070.12926835360827318No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1920.11990108160767367No Hit
GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.11053380960707417No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1760.1099093248070342No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1730.1080358704069143No Hit
GCAGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1730.1080358704069143No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1730.1080358704069143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA150.00460429958.23883458
TAGCGTC150.00460429958.23883458
CTCATAC150.00460429958.23883456
ACCAATG453.092282E-1158.2388358
AACCAAT501.8189894E-1258.23882757
TATTGCA201.9647642E-458.23882754
GTAAGGT201.9647642E-458.23882758
TTATTGC201.9647642E-458.23882753
AGTAAGG201.9647642E-458.23882757
GATGAAG150.0046100458.22051251
ATAGTAC150.0046100458.22051251
CATTGTC201.9678239E-458.22050552
CGGTTTA201.9678239E-458.22050551
TATATTG150.004615786558.20220648
ATATTGT150.004615786558.20220649
ATCGGTT201.9708874E-458.202249
GTATATT150.004621538358.18390347
ACAGTTC150.004638825558.1290845
GATAATC303.683981E-758.1290845
ATACTCT201.9831792E-458.1290846
Produced by FastQC (version 0.11.5)