FastQCFastQC Report
Fri 17 Jun 2016
SRR1526609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156329
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.31216217080644026No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.2782593120918064No Hit
GCAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3930.2513928957519078No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2950.18870459095881123No Hit
GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2720.17399202962981916No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2680.17143332331173358No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.16759526383460524No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2500.15991914488034847No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1980.126655962745236No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1880.12025919695002206No Hit
GCAGGAGGGAAAGCGAAGGCATCGATTACATAAGTTCCTATGTCTCAGAGTCGGAAAACCAATG1680.10746566535959419No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1600.10234825272342303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAGC150.004554917658.3968934
ACGAAGA258.324143E-658.35910833
GCCTGAT150.004584190458.3025238
ACTCCTA150.004584190458.3025238
TGCTTAC150.00459006258.28368435
ATAACTC150.004619502558.1896831
CCCTATA201.9822903E-458.1334258
ACCAATG351.5996193E-858.1334258
TGGCTAT150.004637234458.1334232
ATATTGT258.533962E-658.11469349
TGATCCC150.00464315658.1146947
ACTATAA150.00464315658.1146949
TGATACT150.00464315658.1146942
TGATACG150.00464315658.1146942
CACTATA150.00464315658.1146948
CAAATCA150.00464315658.1146948
CCGACCA150.00464315658.1146941
CCAAATC150.00464315658.1146947
GTTGGGA150.00464315658.1146948
ATCGGTT201.985452E-458.11468549
Produced by FastQC (version 0.11.5)