##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526609_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156329 Sequences flagged as poor quality 0 Sequence length 64 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.94035015895963 39.0 38.0 40.0 35.0 40.0 2 37.42286459965841 39.0 38.0 39.0 33.0 40.0 3 37.406572037178 39.0 38.0 39.0 33.0 40.0 4 37.64519059163687 39.0 38.0 39.0 35.0 40.0 5 37.70679784301058 39.0 38.0 39.0 35.0 40.0 6 36.41396669843727 38.0 35.0 39.0 31.0 40.0 7 38.05927243185845 39.0 38.0 40.0 35.0 40.0 8 37.953962476571846 39.0 38.0 40.0 35.0 40.0 9 37.39105988012461 39.0 36.0 40.0 32.0 40.0 10 36.46650973267916 39.0 35.0 39.0 31.0 40.0 11 36.414491233232475 39.0 35.0 39.0 31.0 40.0 12 36.52318507762475 38.0 35.0 39.0 31.0 40.0 13 36.5898137901477 38.0 35.0 39.0 31.0 40.0 14 36.62803446577411 38.0 35.0 39.0 31.0 40.0 15 36.45576956290899 38.0 36.0 39.0 31.0 40.0 16 36.2667771174894 38.0 35.0 39.0 31.0 40.0 17 35.89353223010446 38.0 35.0 39.0 30.0 40.0 18 36.093578286818186 38.0 35.0 39.0 30.0 40.0 19 36.02639305567105 38.0 35.0 39.0 30.0 40.0 20 35.89524016657178 38.0 35.0 39.0 29.0 40.0 21 35.84851179243774 38.0 35.0 39.0 30.0 40.0 22 35.682336610609674 38.0 35.0 39.0 29.0 40.0 23 35.5399637943056 38.0 35.0 39.0 29.0 40.0 24 35.377767400802156 38.0 34.0 39.0 29.0 40.0 25 35.30523447345023 38.0 34.0 39.0 29.0 40.0 26 35.58529127673049 38.0 35.0 39.0 29.0 40.0 27 35.0526581760262 38.0 35.0 39.0 27.0 40.0 28 34.93895566401628 38.0 34.0 39.0 27.0 40.0 29 34.847187661918134 38.0 34.0 39.0 27.0 40.0 30 34.78385328377972 38.0 34.0 39.0 27.0 40.0 31 34.44660939429025 38.0 34.0 39.0 25.0 40.0 32 34.60910643578607 38.0 34.0 39.0 26.0 40.0 33 34.46132835238503 38.0 33.0 39.0 26.0 40.0 34 33.90550057890731 38.0 33.0 39.0 23.0 40.0 35 33.93126675153042 38.0 33.0 39.0 23.0 40.0 36 33.48072974304192 38.0 33.0 39.0 21.0 40.0 37 33.24539912620179 37.0 32.0 39.0 20.0 40.0 38 33.180574301633094 37.0 32.0 39.0 21.0 40.0 39 32.72287931221974 37.0 32.0 39.0 17.0 40.0 40 32.77565902679605 37.0 32.0 39.0 18.0 40.0 41 32.30121090776503 36.0 31.0 39.0 12.0 40.0 42 32.032060590165614 36.0 31.0 39.0 9.0 40.0 43 31.91773759187355 36.0 31.0 39.0 10.0 40.0 44 31.765136347062924 36.0 30.0 39.0 5.0 40.0 45 31.46563337576521 36.0 30.0 39.0 4.0 40.0 46 31.015441792629648 36.0 30.0 39.0 4.0 40.0 47 30.861791478228607 35.0 29.0 39.0 4.0 40.0 48 30.62979357636779 35.0 29.0 39.0 4.0 40.0 49 30.42005002270852 35.0 29.0 39.0 4.0 40.0 50 30.02571499849676 35.0 28.0 39.0 4.0 40.0 51 29.475292492115987 35.0 27.0 39.0 4.0 39.0 52 29.29423843304825 35.0 27.0 39.0 4.0 39.0 53 29.016043088614396 35.0 26.0 39.0 4.0 39.0 54 28.74272847648229 34.0 25.0 38.0 4.0 39.0 55 28.407410013497177 34.0 24.0 38.0 4.0 39.0 56 27.706650717397284 34.0 20.0 38.0 4.0 39.0 57 27.238126003492635 33.0 19.0 38.0 4.0 39.0 58 26.87830792751185 33.0 16.0 38.0 4.0 39.0 59 26.52154750558118 33.0 12.0 37.0 4.0 39.0 60 26.213818293470823 33.0 4.0 37.0 4.0 39.0 61 25.69885305989292 33.0 4.0 37.0 4.0 39.0 62 25.39663146313224 33.0 4.0 37.0 4.0 39.0 63 25.16109614978667 32.0 4.0 37.0 4.0 39.0 64 24.95925899865028 32.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.10595032122611059 2 2 -0.4144934647813443 2 3 -0.9577682863211976 2 4 0.08250990048582452 2 5 -0.3760968369456563 2 6 -0.35628810188683246 2 7 -0.0839316634517786 2 8 0.16080678028418305 2 9 -0.26383064917331467 2 10 -0.15968814995897418 2 11 -1.1422821452439749 2 12 -0.9736606262945458 2 13 -0.2672190672001449 2 14 -0.5857417589741516 2 15 -0.03836557956949349 2 16 -0.25615944122026235 2 17 0.3785795343071712 2 18 -0.31155475489209294 2 19 -0.5015377983124552 2 20 0.16910402593993723 2 21 -0.12191282956347749 2 22 -0.8924307978656003 2 23 -0.6052527824973026 2 24 -0.2834849342138739 2 25 -1.153534109507774 2 26 -1.0371409009887316 2 27 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119 35 0.6699637595667625 119 36 0.5389808428456035 119 37 0.8095990231378636 119 38 0.13515142662748048 119 39 0.299493423222998 119 40 0.6488312287820008 119 41 1.030751071769096 119 42 0.5773292944726478 119 43 0.8798843098685651 119 44 0.7334724826544274 119 45 0.5154083690442981 119 46 0.18913219303524187 119 47 0.6164890155602052 119 48 0.7768620774144459 119 49 0.6926110653789124 119 50 1.4420343431493912 119 51 1.223357181595368 119 52 1.4916376178240043 119 53 1.1397767171624906 119 54 1.401228047545569 119 55 1.3312797662898 119 56 1.2373534253456242 119 57 1.611343330376016 119 58 1.446099150752712 119 59 1.9761605080816516 119 60 2.044000516787417 119 61 1.4444942390275273 119 62 0.811665615526941 119 63 0.8076616798347196 119 64 0.7120620154575725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 134.0 5 32.0 6 64.0 7 93.0 8 218.0 9 171.0 10 288.0 11 315.0 12 446.0 13 571.0 14 678.0 15 877.0 16 1033.0 17 1122.0 18 1252.0 19 1404.0 20 1611.0 21 1914.0 22 2135.0 23 2177.0 24 2483.0 25 2550.0 26 2882.0 27 3247.0 28 3651.0 29 4192.0 30 5187.0 31 5928.0 32 7355.0 33 9060.0 34 10872.0 35 13682.0 36 16835.0 37 20377.0 38 20652.0 39 10830.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.3986963224523 0.36845842368880616 0.13817190888330233 0.09467334497559603 8 99.30049790725357 0.4147093834397839 0.18687521599447054 0.0979174933121712 9 98.74879261045615 0.7836038099137077 0.21685036045775258 0.25075321917238647 10 62.59491201248649 25.887711173230816 4.472618644013586 7.044758170269112 11 38.47590658163232 29.276717691535158 15.424521361999375 16.82285436483314 12 38.09657836997614 17.11646591483346 22.82941744653903 21.95753826865137 13 27.978813911686252 16.3373398409764 28.07860345809159 27.60524278924576 14 24.688957263207723 19.480070876165012 31.00896186887909 24.822009991748175 15 28.562831699775295 19.433685652644314 28.697912332029475 23.305570315550916 16 30.751561220311217 21.362484643734643 23.96089271089271 23.925061425061426 17 31.179868832300045 22.011579648511553 21.58502843674745 25.223523082440945 18 31.202144195894554 22.878032866582657 21.855190015927946 24.064632921594843 19 29.443673278790243 23.770381695015 22.510218833357854 24.275726192836903 20 29.280700294989025 23.712382500303946 22.386531607338252 24.620385597368774 21 29.30422378445458 21.8148903914181 24.93587242290298 23.94501340122434 22 28.318482175412115 20.742792444140242 26.394335024211756 24.544390356235887 23 30.68144746016414 20.909748031395324 24.809856136737267 23.598948371703266 24 30.709492525092273 22.63198633598383 23.42009813015359 23.238423008770305 25 28.67657412029748 20.805012608322773 24.12669764345967 26.391715627920075 26 26.48265095239009 19.18536576002457 27.35602177988496 26.97596150770038 27 24.545763341900006 18.51619229512084 29.244188654818593 27.69385570816056 28 24.261370916079436 20.32479180012812 28.436258808456117 26.977578475336323 29 25.88237554518032 20.387982656701315 28.652307849956127 25.077333948162238 30 24.794437145512223 19.011119300157016 32.36709712564489 23.82734642868587 31 26.755389734023115 17.94141969729023 34.06478578892372 21.238404779762934 32 27.602931942540078 17.08932942065943 34.828350654176866 20.479387982623628 33 26.69538516766348 17.45674596306339 36.70326993467823 19.144598934594896 34 22.925520305057326 18.22255318602591 40.34177719003171 18.510149318885052 35 21.971299965375295 22.185460187999333 38.71555162287282 17.12768822375255 36 22.922562552541567 22.832720482066883 36.05169769426743 18.19301927112412 37 24.18288487213721 22.532607579336553 34.98639211256034 18.298115435965904 38 24.52809601745772 21.48198068097943 36.0469818041783 17.942941497384552 39 25.153224145035026 19.786516275236917 36.023125257519574 19.037134322208487 40 24.281119392923863 18.562816586737544 36.55746229849194 20.598601721846652 41 21.436003493988284 19.83352173466242 37.17693453910184 21.553540232247457 42 20.24779307285271 18.614056181003388 37.62738454218719 23.510766203956717 43 20.397587727757845 17.186796795638365 39.943936529979815 22.471678946623978 44 22.000873968588543 17.32899776366861 38.87039559931111 21.79973266843174 45 22.523866156664845 18.160779145639804 37.92742116936132 21.38793352833403 46 21.119578148612586 19.592939133789912 37.262467444776696 22.02501527282081 47 20.74979101022442 18.47919747926178 38.69847598225194 22.072535528261845 48 21.980198019801982 19.47023273755947 39.074193133599074 19.475376109039473 49 23.220520998784636 18.6381495604756 37.600396118552624 20.54093332218714 50 20.259797105927184 18.702044389515425 38.20935681548999 22.828801689067408 51 19.794554471366915 20.33164594415535 38.441598538596615 21.43220104588112 52 20.31304280696337 20.495104283269644 37.992305811814056 21.199547097952934 53 21.288794795400285 19.604120951602628 38.54994498104903 20.557139271948056 54 20.609164489853693 19.891781297852354 37.712480537361834 21.78657367493212 55 21.36232872745059 19.896898591204845 35.26814732818041 23.472625353164158 56 20.739596408226323 21.603424415049403 33.85214508705867 23.804834089665604 57 22.46566172779115 23.057168235006372 33.36572995378654 21.111440083415932 58 21.824423105409252 25.00982153782741 32.900541633659856 20.26521372310348 59 23.057452015973208 24.272832667783074 32.91768646141956 19.752028854824168 60 25.23085052227313 23.536765321837525 30.639164362994325 20.593219792895024 61 23.19768789103736 23.020675094621385 32.29614562681841 21.485491387522853 62 21.604481215594117 22.617261693976758 32.093487428773784 23.684769661655345 63 21.43427423508808 22.625424951066243 31.273823014319564 24.666477799526117 64 21.919247252924308 22.801533851061773 30.972190893564918 24.307028002449005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.5 8 5.0 9 6.0 10 24.0 11 41.0 12 67.0 13 120.0 14 147.0 15 175.0 16 445.5 17 688.0 18 1017.0 19 1346.0 20 1526.0 21 1838.5 22 1971.0 23 2456.0 24 3410.0 25 3879.0 26 4111.5 27 4574.0 28 4804.0 29 4648.0 30 4092.0 31 3692.0 32 3351.5 33 3011.0 34 2691.5 35 2116.0 36 1860.0 37 1866.5 38 1943.5 39 2014.0 40 2058.0 41 2117.0 42 2132.0 43 2135.0 44 2138.0 45 2228.0 46 2558.0 47 2798.0 48 2933.5 49 2987.0 50 2905.0 51 3293.5 52 3814.0 53 3946.0 54 4253.5 55 5308.5 56 6056.0 57 6568.5 58 7081.0 59 7352.5 60 8821.0 61 10018.0 62 10474.5 63 10743.0 64 10555.0 65 9197.5 66 7257.5 67 6675.0 68 5990.0 69 5305.0 70 4560.0 71 3304.5 72 2794.0 73 2433.0 74 1848.0 75 1624.0 76 1374.5 77 913.0 78 701.0 79 557.5 80 325.0 81 236.0 82 185.0 83 134.0 84 108.0 85 72.0 86 62.0 87 42.5 88 19.0 89 15.0 90 10.5 91 3.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.001279353159042788 8 0.04797574346410455 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08059924901969565 16 0.026226739760377153 17 0.12345757984762905 18 0.0 19 0.0 20 0.033263182135112486 21 0.0 22 0.0 23 0.0 24 0.0038380594771283637 25 0.05309315610027571 26 0.02430771002181297 27 0.1906236206973754 28 0.14648593671039922 29 0.12025919695002206 30 0.18806491437928982 31 0.2162106838782312 32 0.16311752777795546 33 0.21237262440110283 34 0.35502050163437365 35 0.2373200110024372 36 0.31983828976069695 37 0.3435063232029886 38 0.3351905276692105 39 0.6403162561009154 40 0.362056944009109 41 0.4049152748370424 42 0.5091825572990296 43 0.5059841744014226 44 0.4586481075168395 45 0.49510967254955895 46 0.5270935015256286 47 0.5238951186280216 48 0.5047048212423798 49 0.5245347952075431 50 0.6249640181924019 51 0.5514012115474416 52 0.5673931260354764 53 0.5936198657958536 54 0.5776279513078189 55 0.6064133973862815 56 0.6230449884538377 57 0.6160085460791024 58 0.6767778211336348 59 0.6838142635083702 60 0.7305106538134319 61 0.6224053118743164 62 0.6486320516346935 63 0.6505510813732577 64 0.7445835385629026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 156329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54092970594068 #Duplication Level Percentage of deduplicated Percentage of total 1 80.14934289127838 42.91270333719272 2 8.033452807646356 8.602370641403706 3 3.7025089605734767 5.947073159810399 4 2.099163679808841 4.495647000876357 5 1.4026284348864995 3.7549015217905826 6 0.953405017921147 3.0627714627484344 7 0.7156511350059738 2.682163897933205 8 0.5197132616487455 2.226074496734451 9 0.38829151732377537 1.8710539951000773 >10 1.896057347670251 17.10815011929968 >50 0.10633213859020312 3.9474441722265228 >100 0.03345280764635603 3.389646194883867 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 488 0.31216217080644026 No Hit GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.2782593120918064 No Hit GCAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 393 0.2513928957519078 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 295 0.18870459095881123 No Hit GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG 272 0.17399202962981916 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 268 0.17143332331173358 No Hit GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 262 0.16759526383460524 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 250 0.15991914488034847 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 198 0.126655962745236 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 188 0.12025919695002206 No Hit GCAGGAGGGAAAGCGAAGGCATCGATTACATAAGTTCCTATGTCTCAGAGTCGGAAAACCAATG 168 0.10746566535959419 No Hit GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 160 0.10234825272342303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0019190297385641819 0.0 0.0 0.0 0.0 7 0.0019190297385641819 0.0 0.0 0.0 0.0 8 0.002558706318085576 0.0 0.0 0.0 0.0 9 0.0031983828976069697 0.0 0.0 0.0 0.0 10 0.0038380594771283637 0.0 0.0 0.0 0.0 11 0.00959514869282091 0.0 0.0 0.0 0.0 12 0.013433208169949274 0.0 0.0 0.0 0.0 13 0.014072884749470667 0.0 0.0 0.0 0.0 14 0.014712561328992062 0.0 0.0 0.0 0.0 15 0.015352237908513455 0.0 0.0 0.0 0.0 16 0.015352237908513455 0.0 0.0 0.0 0.0 17 0.017271267647077638 0.0 0.0 0.0 0.0 18 0.018550620806120424 0.0 0.0 0.0 0.0 19 0.021109327124206 0.0 0.0 0.0 0.0 20 0.023668033442291576 0.0 0.0 0.0 0.0 21 0.02430771002181297 0.0 0.0 0.0 0.0 22 0.03070447581702691 0.0 0.0 0.0 0.0 23 0.03134415239654831 0.0 0.0 0.0 0.0 24 0.03390285871463388 0.0 0.0 0.0 0.0 25 0.03518221187367667 0.0 0.0 0.0 0.0 26 0.03902027135080503 0.0 0.0 0.0 0.0 27 0.041578977668890606 0.0 0.0 0.0 0.0 28 0.04477736056649758 0.0 0.0 0.0 0.0 29 0.04797574346410455 0.0 0.0 0.0 0.0 30 0.05437250925931849 0.0 0.0 0.0 0.0 31 0.05757089215692546 0.0 0.0 0.0 0.0 32 0.05948992189548964 0.0 0.0 0.0 0.0 33 0.06268830479309662 0.0 0.0 0.0 0.0 34 0.063327981372618 0.0 0.0 0.0 0.0 35 0.07228345348591753 0.0 0.0 0.0 0.0 36 0.07548183638352449 0.0 0.0 0.0 0.0 37 0.07868021928113146 0.0 0.0 0.0 0.0 38 0.0837976319173026 0.0 0.0 0.0 0.0 39 0.08571666165586679 0.0 0.0 0.0 0.0 40 0.08891504455347377 0.0 0.0 0.0 0.0 41 0.0959514869282091 0.0 0.0 0.0 0.0 42 0.09978954640533746 0.0 0.0 0.0 0.0 43 0.10362760588246582 0.0 0.0 0.0 0.0 44 0.10554663562103 0.0 0.0 0.0 0.0 45 0.10810534193911558 0.0 0.0 0.0 0.0 46 0.11258307799576533 0.0 0.0 0.0 0.0 47 0.11514178431385091 0.0 0.0 0.0 0.0 48 0.1177004906319365 0.0 0.0 0.0 0.0 49 0.12217822668858624 0.0 0.0 0.0 0.0 50 0.12345757984762903 0.0 0.0 0.0 0.0 51 0.1260162861657146 0.0 0.0 0.0 0.0 52 0.1285749924838002 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGAGC 15 0.0045549176 58.39689 34 ACGAAGA 25 8.324143E-6 58.359108 33 GCCTGAT 15 0.0045841904 58.30252 38 ACTCCTA 15 0.0045841904 58.30252 38 TGCTTAC 15 0.004590062 58.283684 35 ATAACTC 15 0.0046195025 58.18968 31 CCCTATA 20 1.9822903E-4 58.13342 58 ACCAATG 35 1.5996193E-8 58.13342 58 TGGCTAT 15 0.0046372344 58.13342 32 ATATTGT 25 8.533962E-6 58.114693 49 TGATCCC 15 0.004643156 58.11469 47 ACTATAA 15 0.004643156 58.11469 49 TGATACT 15 0.004643156 58.11469 42 TGATACG 15 0.004643156 58.11469 42 CACTATA 15 0.004643156 58.11469 48 CAAATCA 15 0.004643156 58.11469 48 CCGACCA 15 0.004643156 58.11469 41 CCAAATC 15 0.004643156 58.11469 47 GTTGGGA 15 0.004643156 58.11469 48 ATCGGTT 20 1.985452E-4 58.114685 49 >>END_MODULE