FastQCFastQC Report
Fri 17 Jun 2016
SRR1526608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142290
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6420.45119122917984394No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5980.4202684658092628No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.3570173589148921No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3900.27408812987560616No Hit
GCAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3680.25862674819031556No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.2515988474242744No Hit
GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2680.18834774052990372No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2420.17007519853819664No Hit
GCAGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2400.1686696183849884No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2390.16796682830838428No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2360.1658584580785719No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1830.12861058401855366No Hit
GCAGGAGGGAAAGCGAAGGCATCGATTACATAAGTTCCTATGTCTCAGAGTCGGAA1760.12369105348232483No Hit
GCAGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1730.12158268325251247No Hit
GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.11244641225665894No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1440.10120177103099305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAG150.00804682350.57015634
AGGCGAT203.9442745E-450.57015634
TCGATGA150.00804682350.57015636
ATCGATG150.00804682350.57015635
TCATCGA150.00813809750.42613233
TAAGTTC354.9058144E-850.4261331
TATTCTC150.0083113150.15828746
ACTCGCC301.0188487E-650.15828740
CTCTGAT150.0083113150.15828739
TCTTTCG150.0083113150.15828746
CGCATCA150.00832295350.1405343
ATGAGTG204.1139804E-450.1405343
TAACACA252.0438867E-550.1405344
TTCGCAC204.1139804E-450.1405345
TCTATTC150.00832295350.1405344
ATGACAA204.1211752E-450.1227938
TCACTAG150.0083346150.1227942
AGTACTC150.0083346150.1227938
CGCACTG150.008381356550.05194541
ATAAGTT355.290167E-849.94605330