##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142290 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.1157495256167 39.0 38.0 40.0 35.0 40.0 2 37.539004849251526 39.0 38.0 40.0 33.0 40.0 3 37.47022981235505 39.0 38.0 40.0 33.0 40.0 4 37.85285684166139 39.0 38.0 40.0 35.0 40.0 5 37.7086091784384 39.0 38.0 40.0 35.0 40.0 6 36.58966898587392 39.0 35.0 39.0 31.0 40.0 7 38.122728231077375 39.0 38.0 40.0 36.0 40.0 8 38.370187644950455 39.0 38.0 40.0 36.0 40.0 9 37.639405439595194 39.0 38.0 40.0 32.0 40.0 10 36.6902733853398 39.0 35.0 40.0 31.0 40.0 11 36.851725349638066 39.0 35.0 40.0 32.0 40.0 12 37.00203106332138 39.0 36.0 40.0 32.0 40.0 13 37.11693021294539 39.0 36.0 40.0 32.0 40.0 14 37.20669056152927 39.0 36.0 40.0 33.0 40.0 15 37.22033171691616 39.0 36.0 40.0 33.0 40.0 16 37.23000913627099 39.0 37.0 40.0 33.0 40.0 17 37.07442546911238 39.0 36.0 40.0 33.0 40.0 18 36.97606999789163 38.0 36.0 40.0 33.0 40.0 19 36.86833227914822 38.0 36.0 39.0 33.0 40.0 20 36.79910042870195 38.0 36.0 40.0 32.0 40.0 21 36.6056082648113 38.0 36.0 39.0 32.0 40.0 22 36.4663504111322 38.0 36.0 39.0 31.0 40.0 23 36.29565675732659 38.0 35.0 39.0 31.0 40.0 24 36.13982008574039 38.0 35.0 39.0 30.0 40.0 25 36.07392648815799 38.0 35.0 39.0 30.0 40.0 26 35.5082156159955 38.0 35.0 39.0 29.0 40.0 27 35.311968515004565 38.0 35.0 39.0 29.0 40.0 28 35.091165928737084 38.0 34.0 39.0 28.0 40.0 29 34.9587743341064 38.0 34.0 39.0 27.0 40.0 30 34.73305924520346 38.0 33.0 39.0 27.0 40.0 31 34.02652329749104 38.0 33.0 39.0 25.0 40.0 32 33.913992550425185 38.0 33.0 39.0 24.0 40.0 33 33.61437908496732 37.0 33.0 39.0 23.0 40.0 34 33.83136552111884 37.0 33.0 39.0 24.0 40.0 35 33.57455899922693 37.0 33.0 39.0 23.0 40.0 36 33.25906950593858 37.0 33.0 39.0 20.0 40.0 37 32.80574179492586 37.0 32.0 39.0 16.0 40.0 38 32.76871881369035 36.0 32.0 39.0 17.0 40.0 39 32.621751352870895 36.0 32.0 39.0 16.0 40.0 40 32.24349567784103 36.0 31.0 39.0 10.0 40.0 41 31.609712558858668 36.0 30.0 39.0 4.0 40.0 42 31.47791130789233 36.0 30.0 39.0 4.0 40.0 43 31.23050108932462 36.0 30.0 39.0 4.0 40.0 44 30.811581980462435 35.0 29.0 39.0 4.0 40.0 45 30.521561599550214 35.0 29.0 39.0 4.0 40.0 46 29.815777637219764 35.0 28.0 39.0 4.0 40.0 47 29.601117436221802 35.0 28.0 39.0 4.0 40.0 48 29.143790849673202 35.0 26.0 38.0 4.0 40.0 49 29.001707779886146 35.0 26.0 38.0 4.0 40.0 50 28.586604821139925 34.0 25.0 38.0 4.0 40.0 51 27.739243797877574 34.0 22.0 38.0 4.0 39.0 52 27.430901679668285 34.0 19.0 38.0 4.0 39.0 53 27.072260875676434 33.0 15.0 38.0 4.0 39.0 54 26.679288776442476 33.0 4.0 38.0 4.0 39.0 55 26.169512966476912 33.0 4.0 38.0 4.0 39.0 56 15.52227844542835 19.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.4069436454033948 1 2 -1.9800286608399276 1 3 -2.1109956334117257 1 4 -2.230993971304173 1 5 -2.3718229357164944 1 6 -1.5516418760702422 1 7 -2.2219534734212942 1 8 -2.6211342486233846 1 9 -3.0345180910321403 1 10 -1.2250952254219953 1 11 -0.6996523447700653 1 12 -1.1760761673330293 1 13 -1.1408911582598265 1 14 -1.6345001737197364 1 15 -1.4882247851650234 1 16 -0.8813709056864241 1 17 -1.4148515637576509 1 18 -0.9781464217019362 1 19 -1.8959179313740293 1 20 -1.6159072422519642 1 21 -1.5506319807930993 1 22 -0.9568190796193221 1 23 -1.053265348412225 1 24 -0.9446293126292176 1 25 -1.3265767658500067 1 26 -0.7826265088543067 1 27 -1.2476454930527012 1 28 -1.0185561024548235 1 29 -0.1162563202013942 1 30 -1.1782938003250223 1 31 0.036490878236094204 1 32 -0.14444495216070408 1 33 -0.4646162826972855 1 34 -0.7988391727826709 1 35 0.1041908447554647 1 36 0.7641363395675285 1 37 0.8001084940459151 1 38 0.566237420553648 1 39 -0.12106658015261473 1 40 0.7945450111303316 1 41 -0.15988824009455982 1 42 0.16631339938342649 1 43 0.20871734504340367 1 44 0.5102145884068676 1 45 0.5984626519627128 1 46 1.2972687090073052 1 47 1.0470224892712992 1 48 1.7464608297574884 1 49 1.0176643683878517 1 50 1.3456330621342332 1 51 1.6764373233750085 1 52 1.7859635956491715 1 53 2.0073838986476673 1 54 1.2462012297432246 1 55 2.281308443015586 1 56 2.057316831179822 2 1 -0.18016093905864494 2 2 0.056299244831045314 2 3 -0.5587777782741625 2 4 -0.23879857545914263 2 5 0.31054651964566204 2 6 -0.5619170024037672 2 7 0.2708034047370447 2 8 0.11306002425701678 2 9 0.16402205495326427 2 10 -1.6541071116193962E-4 2 11 0.09540661087283553 2 12 -0.19544730714212477 2 13 0.09347155931457252 2 14 0.03832408231619411 2 15 -0.5758721742722628 2 16 -0.19989982202555723 2 17 -0.19969154129835687 2 18 -0.3334524295626835 2 19 -0.5593654327777315 2 20 -0.19586232366689416 2 21 0.09389356665215587 2 22 -0.08534238113532666 2 23 -0.10351801545321138 2 24 0.40202986760660764 2 25 0.43013294779401434 2 26 0.12242683196545556 2 27 -0.10519743016668315 2 28 0.5553349699763999 2 29 0.7813293058513864 2 30 0.7934074910843023 2 31 1.058388688455075 2 32 0.4118155756326729 2 33 0.7965852894531906 2 34 -0.014672974017933882 2 35 0.6148590087082937 2 36 0.5540296579279982 2 37 1.05294397972812 2 38 1.1395670106715627 2 39 1.0339025383201488 2 40 0.7641688179804191 2 41 1.1738006987038645 2 42 1.2058979024715768 2 43 1.4613282378002843 2 44 1.5947176765595898 2 45 1.21794608823447 2 46 1.3339896522975927 2 47 1.8372526970197534 2 48 1.6300655462089217 2 49 1.0948120382362525 2 50 1.2219385085126753 2 51 1.6540341790740527 2 52 1.4806295136727563 2 53 0.7395006869688352 2 54 0.7407548513041462 2 55 0.7367267473389987 2 56 -0.011015566349655614 3 1 -0.5871871429130806 3 2 0.15439303257457482 3 3 -0.6702098005395598 3 4 -0.23780083350671788 3 5 -0.043599360730326 3 6 -1.4094725346203276 3 7 -0.10728828249710887 3 8 -0.17276678874513607 3 9 -0.722287875204799 3 10 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2200.0 25 2583.0 26 2533.0 27 2902.0 28 3187.0 29 3596.0 30 4339.0 31 4926.0 32 5957.0 33 7572.0 34 9803.0 35 12546.0 36 16887.0 37 21848.0 38 20102.0 39 9842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.29744365132389 0.39734167868068504 0.20746158444389745 0.09775308555153134 8 99.51999437767938 0.3626396795277251 0.07098179773701595 0.04638414505587181 9 98.80947361023263 0.7210626185958254 0.23824583596879614 0.2312179352027549 10 63.15201349356947 25.35666596387659 4.466230936819173 7.025089605734768 11 38.338604258907864 28.88748330873568 15.439595192915876 17.33431723944058 12 37.51071754866822 17.072879330943845 23.16747487525476 22.24892824513318 13 27.510014758591613 16.43193478108089 28.02515988474243 28.032890575585075 14 24.793731112516692 19.164382598917705 31.061915805748825 24.979970482816782 15 28.072949609951507 19.26980111040832 29.318293625694004 23.338955653946165 16 30.41324056504322 21.014126080539743 24.24414927261227 24.328484081804767 17 31.35497926769274 21.85185185185185 21.63468971818118 25.158479162274226 18 31.12305854241338 22.423922974207606 22.116803710731606 24.33621477264741 19 29.505938576147305 23.400801180687328 22.488579661255184 24.60468058191018 20 28.988685079766675 23.237753882915175 23.132335371424556 24.6412256658936 21 29.149624007309015 21.569330241056996 25.082577834000986 24.198467917633003 22 28.09543889240284 20.528498137606295 26.490969147515635 24.885093822475227 23 30.49968374446553 20.60721062618596 24.846440368261998 24.046665261086513 24 30.147585916086868 22.59188980251599 23.850586829713965 23.409937451683184 25 28.739260654977013 20.956311430459664 23.961218836565095 26.34320907799823 26 26.563159227069445 19.309200348775068 27.379546029870898 26.74809439428459 27 25.013542699957085 17.984705543009504 29.159367679027458 27.84238407800595 28 24.52457170999402 20.429873008055722 28.304077109789986 26.74147817216027 29 26.43347292113876 20.01477988527994 28.646936692824713 24.90481050075659 30 24.962843477709608 18.619820099037103 32.22227700803708 24.195059415216214 31 27.436545898994737 17.638232372686737 33.486435651236675 21.43878607708185 32 28.100303844228726 17.190110610578856 33.69780541279812 21.011780132394307 33 27.46140663897489 17.392929428275234 35.25835866261398 19.887305270135897 34 24.01667266148996 18.196051879906058 39.51152769255725 18.27574776604673 35 22.77129937285278 21.948036373127906 37.909603324091904 17.37106092992741 36 23.54086976159894 22.432142504269763 35.52232275184552 18.50466498228577 37 24.725247330821446 22.411840540174786 34.047251048796646 18.815661080207125 38 24.99947023048506 21.444363605540683 35.12371884072303 18.432447323251232 39 25.821420499491467 19.585546389422532 35.487766979319694 19.105266131766303 40 25.084580213184605 18.473875684781223 35.41185963223533 21.02968446979885 41 22.45799551857952 19.755713104267244 36.06271161281658 21.72357976433666 42 21.143798474275492 18.378687933484635 36.713541335256394 23.763972256983475 43 21.453081554538315 17.149305580121833 38.661817873087074 22.735794992252778 44 22.948232546619014 17.335550758996497 37.505396128940944 22.210820565443544 45 22.99313468752212 18.32684549508104 36.92971901762333 21.750300799773512 46 22.203027428102125 19.456677192336556 35.84061398167684 22.499681397884483 47 21.48196916280848 18.60991943819111 37.33877019354656 22.56934120545385 48 22.44314959736872 19.3546557786095 37.56237949415901 20.639815129862765 49 23.471672974160537 18.62978554520843 36.69614888520361 21.202392595427423 50 21.129315720845852 18.439253925959523 37.18819094052951 23.24323941266512 51 20.48435208343666 20.176140912730183 37.611682266166916 21.72782473766624 52 21.285644417037023 20.43648684089282 36.904331554213535 21.373537187856623 53 22.341715168830063 19.842051907357913 37.29786826788791 20.518364655924117 54 21.652437813767477 19.94710270300933 36.57146098647077 21.82899849675242 55 22.125462720013616 19.70655395918136 34.288084871005715 23.87989844979931 56 21.51995688065587 21.357550140421548 32.897648293665426 24.224844685257153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 3.5 11 5.0 12 11.0 13 63.5 14 110.0 15 116.5 16 123.0 17 157.0 18 191.0 19 464.5 20 738.0 21 1011.5 22 1473.0 23 1661.0 24 1849.5 25 2038.0 26 2454.5 27 2871.0 28 3379.0 29 4200.5 30 4514.0 31 4794.5 32 5075.0 33 4966.5 34 4858.0 35 4295.0 36 3732.0 37 3440.5 38 2660.5 39 2172.0 40 1933.5 41 1695.0 42 1829.5 43 1964.0 44 2048.5 45 2133.0 46 2313.5 47 2809.0 48 3124.0 49 3060.0 50 2996.0 51 3255.5 52 3515.0 53 3796.0 54 4726.0 55 5375.0 56 5748.0 57 6121.0 58 6942.5 59 7764.0 60 8793.5 61 9823.0 62 10226.5 63 10880.5 64 11131.0 65 9924.0 66 8717.0 67 7721.0 68 6725.0 69 5986.0 70 5247.0 71 4518.5 72 3293.5 73 2797.0 74 2407.5 75 2018.0 76 1757.5 77 1497.0 78 1218.5 79 755.0 80 570.0 81 461.5 82 353.0 83 264.5 84 176.0 85 150.5 86 125.0 87 81.5 88 27.5 89 17.0 90 11.0 91 5.0 92 3.5 93 2.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06676505727739124 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03935624428983063 26 0.054817625975121236 27 0.10331014126080539 28 0.10893246187363835 29 0.14407196570384426 30 0.22700119474313024 31 0.23684025581558787 32 0.3099304237824162 33 0.3450699276126221 34 0.35209782837866327 35 0.37739827113641156 36 0.41886288565605456 37 1.328976034858388 38 0.5053060650783611 39 0.4961697940825075 40 0.7056012369105348 41 0.5741794925855647 42 0.5966687750368965 43 0.6669477826973083 44 0.6922482254550566 45 0.7027900766041184 46 0.7365240002811161 47 0.7252793590554502 48 0.8559983133038163 49 0.8349146110056925 50 0.8278867102396513 51 0.8103169583245485 52 0.8496732026143791 53 0.8651345842996696 54 0.8869210766743972 55 0.896760137746855 56 0.9037880385128961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 142290.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.925012298826346 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61825862012509 30.742146320893948 2 10.895903598677101 9.354135919600816 3 5.278496348930875 6.7973856209150325 4 3.017453092766626 5.18096844472556 5 2.1235141949638168 4.557593646777708 6 1.4849864108189528 3.824583596879612 7 0.9856249386030977 2.961557382809755 8 0.803890107731098 2.760559420900977 9 0.615606273944792 2.378241619228336 >10 2.9666983201807526 21.75978635181671 >50 0.14898981630046826 4.473258837585213 >100 0.05566652477160352 3.981305783962331 >500 0.0049117521857297225 1.2284770539039989 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 642 0.45119122917984394 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 598 0.4202684658092628 No Hit GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 508 0.3570173589148921 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 390 0.27408812987560616 No Hit GCAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 368 0.25862674819031556 No Hit GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 358 0.2515988474242744 No Hit GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 268 0.18834774052990372 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 242 0.17007519853819664 No Hit GCAGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 240 0.1686696183849884 No Hit GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 239 0.16796682830838428 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 236 0.1658584580785719 No Hit GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 183 0.12861058401855366 No Hit GCAGGAGGGAAAGCGAAGGCATCGATTACATAAGTTCCTATGTCTCAGAGTCGGAA 176 0.12369105348232483 No Hit GCAGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 173 0.12158268325251247 No Hit GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.11244641225665894 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 144 0.10120177103099305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.003513950383020592 0.0 0.0 0.0 0.0 7 0.003513950383020592 0.0 0.0 0.0 0.0 8 0.003513950383020592 0.0 0.0 0.0 0.0 9 0.003513950383020592 0.0 0.0 0.0 0.0 10 0.004919530536228829 0.0 0.0 0.0 0.0 11 0.010541851149061775 0.0 0.0 0.0 0.0 12 0.011244641225665894 0.0 0.0 0.0 0.0 13 0.011244641225665894 0.0 0.0 0.0 0.0 14 0.014055801532082367 0.0 0.0 0.0 0.0 15 0.014055801532082367 0.0 0.0 0.0 0.0 16 0.01686696183849884 0.0 0.0 0.0 0.0 17 0.020380912221519432 0.0 0.0 0.0 0.0 18 0.022489282451331787 0.0 0.0 0.0 0.0 19 0.02530044275774826 0.0 0.0 0.0 0.0 20 0.026706022910956497 0.0 0.0 0.0 0.0 21 0.02951718321737297 0.0 0.0 0.0 0.0 22 0.032328343523789445 0.0 0.0 0.0 0.0 23 0.032328343523789445 0.0 0.0 0.0 0.0 24 0.033031133600393565 0.0 0.0 0.0 0.0 25 0.035842293906810034 0.0 0.0 0.0 0.0 26 0.037247874060018275 0.0 0.0 0.0 0.0 27 0.03865345421322651 0.0 0.0 0.0 0.0 28 0.04357298474945534 0.0 0.0 0.0 0.0 29 0.044978564902663574 0.0 0.0 0.0 0.0 30 0.05060088551549652 0.0 0.0 0.0 0.0 31 0.05200646566870476 0.0 0.0 0.0 0.0 32 0.05481762597512123 0.0 0.0 0.0 0.0 33 0.05622320612832947 0.0 0.0 0.0 0.0 34 0.05622320612832947 0.0 0.0 0.0 0.0 35 0.06043994658795418 0.0 0.0 0.0 0.0 36 0.06114273666455829 0.0 0.0 0.0 0.0 37 0.06254831681776653 0.0 0.0 0.0 0.0 38 0.06817063743059948 0.0 0.0 0.0 0.0 39 0.07519853819664066 0.0 0.0 0.0 0.0 40 0.08152364888607773 0.0 0.0 0.0 0.0 41 0.0843348091924942 0.0 0.0 0.0 0.0 42 0.08784875957551479 0.0 0.0 0.0 0.0 43 0.08925433972872303 0.0 0.0 0.0 0.0 44 0.09276829011174362 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTAG 15 0.008046823 50.570156 34 AGGCGAT 20 3.9442745E-4 50.570156 34 TCGATGA 15 0.008046823 50.570156 36 ATCGATG 15 0.008046823 50.570156 35 TCATCGA 15 0.008138097 50.426132 33 TAAGTTC 35 4.9058144E-8 50.42613 31 TATTCTC 15 0.00831131 50.158287 46 ACTCGCC 30 1.0188487E-6 50.158287 40 CTCTGAT 15 0.00831131 50.158287 39 TCTTTCG 15 0.00831131 50.158287 46 CGCATCA 15 0.008322953 50.14053 43 ATGAGTG 20 4.1139804E-4 50.14053 43 TAACACA 25 2.0438867E-5 50.14053 44 TTCGCAC 20 4.1139804E-4 50.14053 45 TCTATTC 15 0.008322953 50.14053 44 ATGACAA 20 4.1211752E-4 50.12279 38 TCACTAG 15 0.00833461 50.12279 42 AGTACTC 15 0.00833461 50.12279 38 CGCACTG 15 0.0083813565 50.051945 41 ATAAGTT 35 5.290167E-8 49.946053 30 >>END_MODULE